Rabbit Polyclonal KANK4 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KANK4 aa 350-550.
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- Mechanisms of ageing and development 177:157-1812018Genome-wide profiling of adult human papillary and reticular fibroblasts identifies ACAN, Col XI α1, and PSG1 as general biomarkers of dermis ageing, and KANK4 as an exemplary effector of papillary fibroblast ageing, related to contractility.Applications:Unspecified applicationReactive species:Unspecified reactive speciesValérie Haydont et. al.PubMed 29913199
- Nature communications 8:148982017Genome-wide association study identifies three novel loci in Fuchs endothelial corneal dystrophy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatalie A Afshari et. al.PubMed 28358029
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human KANK4 aa 350-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q5T7N3
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Target data
Function
May be involved in the control of cytoskeleton formation by regulating actin polymerization.
Alternative names
ANKRD38, KANK4, KN motif and ankyrin repeat domain-containing protein 4, Ankyrin repeat domain-containing protein 38
Recommended products
Rabbit Polyclonal KANK4 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KANK4 aa 350-550.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human KANK4 aa 350-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q5T7N3
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
KANK4 also referred to as KN motif and ankyrin repeat domain-containing protein 4 is a protein that plays an important role in cellular functions. It has a molecular mass of approximately 45 kDa. This protein is expressed in various tissues with notable levels in the kidney and brain. Its presence in these tissues suggests a significant role in cellular structural maintenance and signaling processes. KANK4 contributes to the organization of the cytoskeleton and participates in cellular adhesion.
- Biological function summary
KANK4 functions as a scaffolding protein that connects other proteins to the cytoskeleton aiding in maintaining cell shape and motility. It is a part of a larger protein complex that includes elements involved in actin filament assembly. This functional role is important for cellular responses to external stimuli and mechanical stress. KANK4's interaction with such protein complexes can influence cell motility and structural integrity reflecting its versatility in multiple biological contexts.
- Pathways
KANK4 integrates into pathways like the Rho GTPase signaling pathway which is vital for cytoskeletal rearrangement and cell movement. It is associated with actin-binding proteins and Rho-associated coiled-coil containing protein kinase (ROCK) indicating its role in signal transduction and changes in cell shape. These pathways highlight KANK4’s involvement in cellular dynamics and its influence on other proteins related to cytoskeletal functions.
- Associated diseases and disorders
KANK4 has associations with kidney diseases particularly those involving impaired glomerular function. Alterations or dysregulation in KANK4 can disrupt cellular architecture impacting kidney function and leading to pathological states. Additionally deficiencies in KANK4 expression have been linked to neurodevelopmental disorders due to its interactions with proteins like Ankyrin which is essential for neuronal connectivity and stability. These connections indicate the potential impact of KANK4 in specific tissues and highlight its significance in health and disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
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5 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KANK4 antibody (ab121410)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling KANK4 with ab121410 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-KANK4 antibody (ab121410)
All lanes: Western blot - Anti-KANK4 antibody (ab121410) at 0.4 µg/mL
All lanes: Human Placenta Tissue
Predicted band size: 107 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KANK4 antibody (ab121410)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling KANK4 with ab121410 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KANK4 antibody (ab121410)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling KANK4 with ab121410 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KANK4 antibody (ab121410)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling KANK4 with ab121410 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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