AB10483

Anti-KAP1 antibody

Name: Anti-KAP1 antibody (ab10483) | Abcam
Brand: Abcam Limited
SKU: AB10483
Price: 450 USD
Availability: InStock
Rating: 3 (7 reviews)

(7 Reviews)

(86 Publications)

Anti-KAP1 antibody (ab10483) is a rabbit polyclonal antibody detecting KAP1 in Western Blot, IP, IHC-P. Suitable for Human, Mouse, Rat.

- Over 70 publications
- Trusted since 2004

View Alternative Names

KAP1, RNF96, TIF1B, TRIM28, Transcription intermediary factor 1-beta, TIF1-beta, E3 SUMO-protein ligase TRIM28, KRAB-associated protein 1, KRAB-interacting protein 1, Nuclear corepressor KAP-1, RING finger protein 96, RING-type E3 ubiquitin transferase TIF1-beta, Tripartite motif-containing protein 28, KAP-1, KRIP-1

5 Images

Supplier Data

Immunoprecipitation - Anti-KAP1 antibody (AB10483)

Immunoprecipitation of KAP-1 from HeLa whole cell lysate using ab10483

Lane 1 : ab10483 current lot

Lane 2 : ab10483 previous lot

Lane 3 : other rabbit anti-KAP-1 antibody

Lane 4 : Control IgG

All lanes:

Immunoprecipitation - Anti-KAP1 antibody (ab10483)

Predicted band size: 88 kDa

false

Exposure time: 30s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody (AB10483)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody (AB10483)

Immunohistochemistry analysis of formalin-fixed paraffin embedded sections of human breast tissue staining KAP-1 with ab10483 at 1/1000 dilution, showing the current lot on the left, and previous lot on the right.

Collaborator

Immunoprecipitation - Anti-KAP1 antibody (AB10483)

The ab10483 antibody was used to immunoprecipitate KAP1 from HEK293 nuclear extracts. Three different amounts of ab10483 or control IgG were tested (1, 2 or 4 ug). The eluates from these experiments as well as the supernatants from the KAP1 IPs were analyzed by Western blotting using the ab10483 antibody. As shown by Western blotting, the presence of a ~110 kDa band demonstrated that KAP1 was specifically precipitated from these extracts. The 50 and 25 kDa bands correspond to the IgG of the IP antibodies.

All lanes:

Immunoprecipitation - Anti-KAP1 antibody (ab10483)

Predicted band size: 88 kDa

false

This image was taken from an abreview submitted by an Seth Frietze.

Supplier Data

Western blot - Anti-KAP1 antibody (AB10483)

All lanes:

Western blot - Anti-KAP1 antibody (ab10483) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg

Lane 4:

HEK293T cells (human epithelial cell line from embryonic kidney transformed with large T antigen) at 50 µg

Predicted band size: 88 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-KAP1 antibody (AB10483)

All lanes:

Western blot - Anti-KAP1 antibody (ab10483) at 1 µg/mL

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg/mL

Lane 2:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg/mL

Lane 3:

4T1 (Mouse mammary gland carcinoma cell line) whole cell lysate at 50 µg/mL

Lane 4:

CT26.WT (murine colon carcinoma) whole cell lysate at 50 µg/mL

Lane 5:

C6 (rat glioma cell line) whole cell lysate at 50 µg/mL

Predicted band size: 88 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, IP, WB

applications

Immunogen

Synthetic Peptide within Human TRIM28. The exact immunogen used to generate this antibody is proprietary information.

Q13263

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" } } }

style

left-column

Product details

What is this antibody validated in?
Anti-KAP1 antibody (ab10483) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.

What is the molecular weight of KAP1?
Anti-KAP1 (ab10483) specifically detects a band for KAP1 (UniProt: Q13263) at a molecular weight of 100kDa.

Trusted by the scientific community
Anti-KAP1 (ab10483) was first used in a scientific publication in 2004 and has been cited over 70 times in peer-reviewed journals.

Reviewed by scientists
Anti-KAP1 (ab10483) has over 5 independent reviews from customers.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibodies were affinity purified using the peptide immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KAP1 also known as TRIM28 or TIF1Β is a multifunctional protein involved in transcriptional regulation. It has a molecular weight of approximately 87 kDa. This protein is expressed in a wide variety of human tissues where it plays a role as a co-repressor for KRAB domain-containing zinc finger proteins. KAP1 recruits various epigenetic modifiers to silence transcription and this activity depends on its phosphorylation status. You might explore KAP1 using KAP1 ELISA kits and study its post-translational modifications like phosphorylation in various conditions.

Biological function summary

KAP1 acts as a scaffold protein facilitating the assembly of large protein complexes that include chromatin remodelers and histone deacetylases. It is significant in maintaining chromatin structure and mediating gene silencing. KAP1 phosphorylation serves as a switch between its roles in transcriptional repression and activation. This protein also interacts with heterochromatin protein 1 (HP1) and other TRIM family proteins playing an important role in genomic stability by controlling gene expression and repair mechanisms.

Pathways

KAP1 participates actively in p53 and DNA damage response pathways. In the p53 pathway KAP1 regulates genes involved in the cell cycle and apoptosis acting in conjunction with the p53 protein. Within the DNA damage response KAP1 modulates the repair of double-strand breaks by interacting with proteins like ATM kinase influencing cellular sensitivity to genotoxic stress. These pathways highlight its importance in maintaining cellular homeostasis and response to external stimuli.

KAP1 has known connections with cancer and neurological disorders due to its regulatory effects on gene expression and genome stability. In cancer aberrant KAP1 activity can lead to unregulated cell proliferation and survival often involving changes in its interaction with the p53 protein. In neurological disorders dysregulation of KAP1 is associated with impaired neural development and neurodegeneration. Altogether these associations highlight KAP1 as a potential target for therapeutic interventions in related diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also a corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteasomal degradation; the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6. Mediates the nuclear localization of KOX1, ZNF268 and ZNF300 transcription factors. In association with isoform 2 of ZFP90, is required for the transcriptional repressor activity of FOXP3 and the suppressive function of regulatory T-cells (Treg) (PubMed : 23543754). Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed : 24623306). Required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed : 24623306). In ESCs, in collaboration with SETDB1, is also required for H3K9me3 and silencing of endogenous and introduced retroviruses in a DNA-methylation independent-pathway (By similarity). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed : 24623306). The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed : 27029610).. (Microbial infection) Plays a critical role in the shutdown of lytic gene expression during the early stage of herpes virus 8 primary infection. This inhibition is mediated through interaction with herpes virus 8 protein LANA1.

See full target information TRIM28

style

left-column

Publications (86)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113194 PubMed40822359

2025

AURKB and PI3K/AKT/mTOR pathways converge to regulate expression.

Applications

Unspecified application

Species

Unspecified reactive species

Grace Lim,Althea Bastian,Saskia Fung,Farhana Mollah,Minh H Nguyen,Adwoa A Sey,Marthe Cheade,Rozelle Harvie,Nisha R Singh,Sergey Kurdyukov,Kristie-Ann Dickson,Eugene H Y Choi,Ailise C Seery,Matti L Gild,Deborah J Marsh,Hilda A Pickett,Roger R Reddel,Martyn Bullock,Roderick J Clifton-Bligh

The Journal of clinical investigation 135: PubMed40519166

2025

RSK1-driven TRIM28/E2F1 feedback loop promotes castration-resistant prostate cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Miyeong Kim,Jinpeng Liu,Yanquan Zhang,Ruixin Wang,Ryan Goettl,Jennifer Grasso,Derek B Allison,Chi Wang,Tianyan Gao,Xiaoqi Liu,Ka-Wing Fong

Biomarker research 13:57 PubMed40197552

2025

A novel indirubin- 3-monoxime derivative I3MV- 8b exhibits remarkable cytotoxicity against multiple myeloma by targeting TRIM28.

Applications

Unspecified application

Species

Unspecified reactive species

Teng Fang,Lanting Liu,Hao Sun,Xiaoyu Zhang,Xiyue Sun,Zhen Yu,Lixin Gong,Shiyi Xie,Yonglong Zhao,Yan Li,Lugui Qiu,Gang An,Bin He,Mu Hao

eLife 14: PubMed40146629

2025

CDK-mediated phosphorylation of PNKP is required for end-processing of single-strand DNA gaps on Okazaki fragments and genome stability.

Applications

Unspecified application

Species

Unspecified reactive species

Kaima Tsukada,Rikiya Imamura,Tomoko Miyake,Kotaro Saikawa,Mizuki Saito,Naoya Kase,Lingyan Fu,Masamichi Ishiai,Yoshihisa Matsumoto,Mikio Shimada

Nucleic acids research 53: PubMed39921567

2025

Elevated reactive oxygen species can drive the alternative lengthening of telomeres pathway in ATRX-null cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Tomas Goncalves,Siobhan Cunniffe,Tiffany S Ma,Natalie Mattis,Andrew W Rose,Thomas Kent,David R Mole,Helene E B Geiller,Linda van Bijsterveldt,Timothy C Humphrey,Ester M Hammond,Richard J Gibbons,David Clynes,Anna M Rose

Blood 145:422-437 PubMed39437550

2024

Conserved helical motifs in the IKZF1 disordered region mediate NuRD interaction and transcriptional repression.

Applications

Unspecified application

Species

Unspecified reactive species

Tianyi Zhang,Yi-Fang Wang,Alex Montoya,Ilinca Patrascan,Nehir Nebioglu,Husayn A Pallikonda,Radina Georgieva,James W D King,Holger B Kramer,Pavel V Shliaha,David S Rueda,Matthias Merkenschlager

Scientific reports 14:19999 PubMed39198533

2024

Discovery of the DNA-PKcs inhibitor DA-143 which exhibits enhanced solubility relative to NU7441.

Applications

Unspecified application

Species

Unspecified reactive species

Zachary J Waldrip,Baku Acharya,Daniel Armstrong,Maha Hanafi,Randall R Rainwater,Sharon Amole,Madeline Fulmer,Ana Clara Azevedo-Pouly,Alaina Burns,Lyle Burdine,Brendan Frett,Marie Schluterman Burdine

Science advances 10:eadk5386 PubMed38536927

2024

Activation of endogenous retroviruses and induction of viral mimicry by MEK1/2 inhibition in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Alice Cortesi,Francesco Gandolfi,Fabiana Arco,Pierluigi Di Chiaro,Emanuele Valli,Sara Polletti,Roberta Noberini,Francesco Gualdrini,Sergio Attanasio,Francesca Citron,I-Lin Ho,Rutvi Shah,Er-Yen Yen,Mara Cetty Spinella,Simona Ronzoni,Simona Rodighiero,Nico Mitro,Tiziana Bonaldi,Serena Ghisletti,Silvia Monticelli,Andrea Viale,Giuseppe Riccardo Diaferia,Gioacchino Natoli

Cell research 34:58-75 PubMed38168642

2024

ZNF689 deficiency promotes intratumor heterogeneity and immunotherapy resistance in triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Ping Ge,Xi Jin,Ding Ma,Zi-Yu Wang,Cheng-Lin Liu,Chao-Zheng Zhou,Shen Zhao,Tian-Jian Yu,Xi-Yu Liu,Gen-Hong Di,Zhi-Ming Shao,Yi-Zhou Jiang

Nucleic acids research 52:1136-1155 PubMed38038252

2023

DAXX promotes centromeric stability independently of ATRX by preventing the accumulation of R-loop-induced DNA double-stranded breaks.

Applications

Unspecified application

Species

Unspecified reactive species

Lia M Pinto,Alexandros Pailas,Max Bondarchenko,Abhishek Bharadwaj Sharma,Katrin Neumann,Anthony J Rizzo,Céline Jeanty,Nathalie Nicot,Carine Racca,Mindy K Graham,Catherine Naughton,Yaqun Liu,Chun-Long Chen,Paul J Meakin,Nick Gilbert,Sébastien Britton,Alan K Meeker,Christopher M Heaphy,Florence Larminat,Eric Van Dyck

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KAP1 antibody

Immunoprecipitation - Anti-KAP1 antibody (AB10483)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody (AB10483)

Immunoprecipitation - Anti-KAP1 antibody (AB10483)

Western blot - Anti-KAP1 antibody (AB10483)

Western blot - Anti-KAP1 antibody (AB10483)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (86)

Product promise