Rabbit Polyclonal KAP1 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat samples. Cited in 72 publications. Immunogen corresponding to Synthetic Peptide within Human TRIM28.
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- eLife 12:2023ZMYM2 controls human transposable element transcription through distinct co-regulatory complexes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDanielle J Owen,Elisa Aguilar-Martinez,Zongling Ji,Yaoyong Li,Andrew D SharrocksPubMed 37934570
- Histology and histopathology 39:603-6102023Up-regulation of Trim28 in pregnancy-induced hypertension is involved in the injury of human umbilical vein endothelial cells through the p38 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Chen,Peng Shi,Ping Wang,Tingting Zhang,Jing Zhao,Li ZhaoPubMed 37522419
- iScience 26:1071262023Genome-wide CRISPR-Cas9 screen analyzed by SLIDER identifies network of repressor complexes that regulate TRIM24.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLalit R Patel,Sabrina A Stratton,Megan McLaughlin,Patrick Krause,Kendra Allton,Andrés López Rivas,Daniela Barbosa,Traver Hart,Michelle C BartonPubMed 37426340
- The EMBO journal 41:e1111792022Structure and functional mapping of the KRAB-KAP1 repressor complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuido A Stoll,Ninoslav Pandiloski,Christopher H Douse,Yorgo ModisPubMed 36341546
- Investigative ophthalmology & visual science 63:72022UBE3D Is Involved in Blue Light-Induced Retinal Damage by Regulating Double-Strand Break Repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNingda Xu,Yue Liu,Shanshan Nai,Yong Tao,Yuehe Ding,Lemei Jia,Qizhi Geng,Jie Li,Yujing Bai,Gong-Hong Wei,Meng-Qiu Dong,Linyi Luo,Mingwei Zhao,Xingzhi Xu,Xiao-Xin Li,Jing Li,Lvzhen HuangPubMed 36094642
- EJNMMI research 12:502022Radiofluorination of a highly potent ATM inhibitor as a potential PET imaging agent.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClaudia Rose Fraser,Javier Ajenjo,Mathew Veal,Gemma Marie Dias,Chung Chan,Edward O'Neill,Gianluca Destro,Doreen Lau,Anna Pacelli,Veronique Gouverneur,Rebekka Hueting,Bart CornelissenPubMed 35962885
- Epigenetics & chromatin 15:272022The mismatch-repair proteins MSH2 and MSH6 interact with the imprinting control regions through the ZFP57-KAP1 complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBasilia Acurzio,Francesco Cecere,Carlo Giaccari,Ankit Verma,Rosita Russo,Mariangela Valletta,Bruno Hay Mele,Claudia Angelini,Angela Chambery,Andrea RiccioPubMed 35918739
- Science advances 8:eabk00132022Genome-wide screening identifies Polycomb repressive complex 1.3 as an essential regulator of human naïve pluripotent cell reprogramming.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmanda J Collier,Adam Bendall,Charlene Fabian,Andrew A Malcolm,Katarzyna Tilgner,Claudia I Semprich,Katarzyna Wojdyla,Paola Serena Nisi,Kamal Kishore,Valar Nila Roamio Franklin,Bahar Mirshekar-Syahkal,Clive D'Santos,Kathrin Plath,Kosuke Yusa,Peter J Rugg-GunnPubMed 35333572
- Retrovirology 18:382021Trim28 acts as restriction factor of prototype foamy virus replication by modulating H3K9me3 marks and destabilizing the viral transactivator Tas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeipei Yuan,Jun Yan,Shuang Wang,Yang Guo,Xueyan Xi,Song Han,Jun Yin,Biwen Peng,Xiaohua He,Jochen Bodem,Wanhong LiuPubMed 34903241
- Communications biology 4:12212021PIM1 phosphorylation of the androgen receptor and 14-3-3 ζ regulates gene transcription in prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSophie E Ruff,Nikita Vasilyev,Evgeny Nudler,Susan K Logan,Michael J GarabedianPubMed 34697370
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human TRIM28. The exact immunogen used to generate this antibody is proprietary information. Database link Q13263
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested |
| Rat | Expected | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Rat | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Associated Products
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Target data
Function
Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteosomal degradation; the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6. Mediates the nuclear localization of KOX1, ZNF268 and ZNF300 transcription factors. In association with isoform 2 of ZFP90, is required for the transcriptional repressor activity of FOXP3 and the suppressive function of regulatory T-cells (Treg) (PubMed:23543754). Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). In ESCs, in collaboration with SETDB1, is also required for H3K9me3 and silencing of endogenous and introduced retroviruses in a DNA-methylation independent-pathway (By similarity). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed:27029610). Acts as a corepressor for ZFP568 (By similarity).(Microbial infection) Plays a critical role in the shutdown of lytic gene expression during the early stage of herpes virus 8 primary infection. This inhibition is mediated through interaction with herpes virus 8 protein LANA1.
Alternative names
Transcription intermediary factor 1-beta, TIF1-beta, E3 SUMO-protein ligase TRIM28, KRAB-associated protein 1, KRAB-interacting protein 1, Nuclear corepressor KAP-1, RING finger protein 96, RING-type E3 ubiquitin transferase TIF1-beta, Tripartite motif-containing protein 28, KAP-1, KRIP-1, TRIM28, KAP1, RNF96, TIF1B
Recommended products
Rabbit Polyclonal KAP1 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat samples. Cited in 72 publications. Immunogen corresponding to Synthetic Peptide within Human TRIM28.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human TRIM28. The exact immunogen used to generate this antibody is proprietary information. Database link Q13263
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
KAP1 also known as TRIM28 or TIF1β is a multifunctional protein involved in transcriptional regulation. It has a molecular weight of approximately 87 kDa. This protein is expressed in a wide variety of human tissues where it plays a role as a co-repressor for KRAB domain-containing zinc finger proteins. KAP1 recruits various epigenetic modifiers to silence transcription and this activity depends on its phosphorylation status. You might explore KAP1 using KAP1 ELISA kits and study its post-translational modifications like phosphorylation in various conditions.
- Biological function summary
KAP1 acts as a scaffold protein facilitating the assembly of large protein complexes that include chromatin remodelers and histone deacetylases. It is significant in maintaining chromatin structure and mediating gene silencing. KAP1 phosphorylation serves as a switch between its roles in transcriptional repression and activation. This protein also interacts with heterochromatin protein 1 (HP1) and other TRIM family proteins playing an important role in genomic stability by controlling gene expression and repair mechanisms.
- Pathways
KAP1 participates actively in p53 and DNA damage response pathways. In the p53 pathway KAP1 regulates genes involved in the cell cycle and apoptosis acting in conjunction with the p53 protein. Within the DNA damage response KAP1 modulates the repair of double-strand breaks by interacting with proteins like ATM kinase influencing cellular sensitivity to genotoxic stress. These pathways highlight its importance in maintaining cellular homeostasis and response to external stimuli.
- Associated diseases and disorders
KAP1 has known connections with cancer and neurological disorders due to its regulatory effects on gene expression and genome stability. In cancer aberrant KAP1 activity can lead to unregulated cell proliferation and survival often involving changes in its interaction with the p53 protein. In neurological disorders dysregulation of KAP1 is associated with impaired neural development and neurodegeneration. Altogether these associations highlight KAP1 as a potential target for therapeutic interventions in related diseases.
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Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Immunoprecipitation - Anti-KAP1 antibody (ab10483)
Immunoprecipitation of KAP-1 from HeLa whole cell lysate using ab10483
Lane 1: ab10483 current lot
Lane 2: ab10483 previous lot
Lane 3: other rabbit anti-KAP-1 antibody
Lane 4: Control IgG
All lanes: Immunoprecipitation - Anti-KAP1 antibody (ab10483)
Predicted band size: 88 kDa
Exposure time: 30s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody (ab10483)
Immunohistochemistry analysis of formalin-fixed paraffin embedded sections of human breast tissue staining KAP-1 with ab10483 at 1/1000 dilution, showing the current lot on the left, and previous lot on the right.
Western blot - Anti-KAP1 antibody (ab10483)
All lanes: Western blot - Anti-KAP1 antibody (ab10483) at 1 µg/mL
Lane 1: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg/mL
Lane 2: TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg/mL
Lane 3: 4T1 (Mouse mammary gland carcinoma cell line) whole cell lysate at 50 µg/mL
Lane 4: CT26.WT (murine colon carcinoma) whole cell lysate at 50 µg/mL
Lane 5: C6 (rat glioma cell line) whole cell lysate at 50 µg/mL
Predicted band size: 88 kDa
Exposure time: 3min
Western blot - Anti-KAP1 antibody (ab10483)
All lanes: Western blot - Anti-KAP1 antibody (ab10483) at 0.1 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4: HEK293T cells (human epithelial cell line from embryonic kidney transformed with large T antigen) at 50 µg
Predicted band size: 88 kDa
Exposure time: 30s
This image was taken from a customer review submitted by an Seth Frietze.Immunoprecipitation - Anti-KAP1 antibody (ab10483)
The ab10483 antibody was used to immunoprecipitate KAP1 from HEK293 nuclear extracts. Three different amounts of ab10483 or control IgG were tested (1, 2 or 4 ug). The eluates from these experiments as well as the supernatants from the KAP1 IPs were analyzed by Western blotting using the ab10483 antibody. As shown by Western blotting, the presence of a ~110 kDa band demonstrated that KAP1 was specifically precipitated from these extracts. The 50 and 25 kDa bands correspond to the IgG of the IP antibodies.
All lanes: Immunoprecipitation - Anti-KAP1 antibody (ab10483)
Predicted band size: 88 kDa
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