Anti-KAP1 antibody (ab10484) is a rabbit polyclonal antibody that is used to detect KAP1 in Western Blot, IP, IHC-P. Suitable for Human, Mouse samples.
- Over 50 publications
- Trusted since 2004
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- Frontiers in molecular biosciences 10:12041242023Long non-coding RNA lncMGC mediates the expression of TGF-β-induced genes in renal cells via nucleosome remodelers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMitsuo Kato,Zhuo Chen,Sadhan Das,Xiwei Wu,Jinhui Wang,Arthur Li,Wei Chen,Walter Tsark,Ragadeepthi Tunduguru,Linda Lanting,Mei Wang,Roger Moore,Markus Kalkum,Maryam Abdollahi,Rama NatarajanPubMed 37325470
- Journal of radiation research 64:485-4952023APTX acts in DNA double-strand break repair in a manner distinct from XRCC4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRikiya Imamura,Mizuki Saito,Mikio Shimada,Junya Kobayashi,Masamichi Ishiai,Yoshihisa MatsumotoPubMed 36940705
- Cells 12:2023Role of C-Terminal Phosphorylation of Lamin A in DNA Damage and Cellular Senescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Ao,Zhuping Wu,Zhiwei Liao,Juncong Lan,Jie Zhang,Pengfei Sun,Baohua Liu,Zimei WangPubMed 36831305
- EMBO reports 24:e549442022The bromodomain protein TRIM28 controls the balance between growth and invasiveness in melanoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWilliam A Nyberg,Diego A Velasquez-Pulgarin,Tianlin He,Maria Sjöstrand,Lucia Pellé,Ruxandra Covacu,Alexander EspinosaPubMed 36341538
- The EMBO journal 41:e1111792022Structure and functional mapping of the KRAB-KAP1 repressor complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuido A Stoll,Ninoslav Pandiloski,Christopher H Douse,Yorgo ModisPubMed 36341546
- Molecular cancer therapeutics 21:245-2562021RP-3500: A Novel, Potent, and Selective ATR Inhibitor that is Effective in Preclinical Models as a Monotherapy and in Combination with PARP Inhibitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnne Roulston,Michal Zimmermann,Robert Papp,Alexander Skeldon,Charles Pellerin,Émilie Dumas-Bérube,Valerie Dumais,Stéphane Dorich,Lee D Fader,Sara Fournier,Li Li,Marie-Eve Leclaire,Shou Yun Yin,Amandine Chefson,Hunain Alam,William Yang,Chloe Fugère-Desjardins,Sabrina Vignini-Hammond,Kathryn Skorey,Amina Mulani,Victoria Rimkunas,Artur Veloso,Martine Hamel,Rino Stocco,Yael Mamane,Zuomei Li,Jordan T F Young,Michael Zinda,W Cameron BlackPubMed 34911817
- Cancer immunology research 9:1413-14242021The SETDB1-TRIM28 Complex Suppresses Antitumor Immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianhuang Lin,Dajiang Guo,Heng Liu,Wei Zhou,Chen Wang,Iris Müller,Andrew V Kossenkov,Ronny Drapkin,Benjamin G Bitler,Kristian Helin,Rugang ZhangPubMed 34848497
- EMBO reports 23:e510412021POGZ promotes homology-directed DNA repair in an HP1-dependent manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohn Heath,Estelle Simo Cheyou,Steven Findlay,Vincent M Luo,Edgar Pinedo Carpio,Jeesan Lee,Billel Djerir,Xiaoru Chen,Théo Morin,Benjamin Lebeau,Martin Karam,Halil Bagci,Damien Grapton,Josie Ursini-Siegel,Jean-Francois Côté,Michael Witcher,Stéphane Richard,Alexandre Maréchal,Alexandre OrthweinPubMed 34758190
- ACS chemical biology 16:1721-17362021A Peptidomimetic Ligand Targeting the Chromodomain of MPP8 Reveals HRP2's Association with the HUSH Complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJarod M Waybright,Sarah E Clinkscales,Kimberly D Barnash,Gabrielle R Budziszewski,Justin M Rectenwald,Anna M Chiarella,Jacqueline L Norris-Drouin,Stephanie H Cholensky,Kenneth H Pearce,Laura E Herring,Robert K McGinty,Nathaniel A Hathaway,Lindsey I JamesPubMed 34415726
- NAR cancer 3:zcab0102021Ubiquitination-mediated degradation of TRDMT1 regulates homologous recombination and therapeutic response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaolan Zhu,Xiangyu Wang,Wei Yan,Haibo Yang,Yufei Xiang,Fengping Lv,Yi Shi,Hong-Yu Li,Li LanPubMed 33778494
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TRIM28 aa 650-700. The exact immunogen used to generate this antibody is proprietary information. Database link Q13263
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Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2-10 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/10000 | Notes - |
Species Human | Dilution info 1/500 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
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Target data
Function
Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also a corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteasomal degradation; the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6. Mediates the nuclear localization of KOX1, ZNF268 and ZNF300 transcription factors. In association with isoform 2 of ZFP90, is required for the transcriptional repressor activity of FOXP3 and the suppressive function of regulatory T-cells (Treg) (PubMed:23543754). Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). In ESCs, in collaboration with SETDB1, is also required for H3K9me3 and silencing of endogenous and introduced retroviruses in a DNA-methylation independent-pathway (By similarity). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed:27029610). (Microbial infection) Plays a critical role in the shutdown of lytic gene expression during the early stage of herpes virus 8 primary infection. This inhibition is mediated through interaction with herpes virus 8 protein LANA1.
Alternative names
KAP1, RNF96, TIF1B, TRIM28, Transcription intermediary factor 1-beta, TIF1-beta, E3 SUMO-protein ligase TRIM28, KRAB-associated protein 1, KRAB-interacting protein 1, Nuclear corepressor KAP-1, RING finger protein 96, RING-type E3 ubiquitin transferase TIF1-beta, Tripartite motif-containing protein 28, KAP-1, KRIP-1
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Anti-KAP1 antibody (ab10484) is a rabbit polyclonal antibody that is used to detect KAP1 in Western Blot, IP, IHC-P. Suitable for Human, Mouse samples.
- Over 50 publications
- Trusted since 2004
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TRIM28 aa 650-700. The exact immunogen used to generate this antibody is proprietary information. Database link Q13263
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-KAP1 antibody (ab10484) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), in Human, Mouse samples.
What is the molecular weight of KAP1?
Anti-KAP1 (ab10484) specifically detects a band for KAP1 (UniProt: Q13263) at a molecular weight of 100kDa.
Recommended positive controls
WB: HeLa, 293T, 3T3 whole cell lysate.IP: HeLa whole cell lysate.IHC-P: Human ovarian carcinoma, mouse plasmacytoma, mouse coronal brain tissue.
Trusted by the scientific community
Anti-KAP1 (ab10484) was first used in a scientific publication in 2004 and has been cited over 50 times in peer-reviewed journals.
Reviewed by scientists
Anti-KAP1 (ab10484) has over 5 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
KAP1 also known as TRIM28 or TIF1β is a multifunctional protein involved in transcriptional regulation. It has a molecular weight of approximately 87 kDa. This protein is expressed in a wide variety of human tissues where it plays a role as a co-repressor for KRAB domain-containing zinc finger proteins. KAP1 recruits various epigenetic modifiers to silence transcription and this activity depends on its phosphorylation status. You might explore KAP1 using KAP1 ELISA kits and study its post-translational modifications like phosphorylation in various conditions.
- Biological function summary
KAP1 acts as a scaffold protein facilitating the assembly of large protein complexes that include chromatin remodelers and histone deacetylases. It is significant in maintaining chromatin structure and mediating gene silencing. KAP1 phosphorylation serves as a switch between its roles in transcriptional repression and activation. This protein also interacts with heterochromatin protein 1 (HP1) and other TRIM family proteins playing an important role in genomic stability by controlling gene expression and repair mechanisms.
- Pathways
KAP1 participates actively in p53 and DNA damage response pathways. In the p53 pathway KAP1 regulates genes involved in the cell cycle and apoptosis acting in conjunction with the p53 protein. Within the DNA damage response KAP1 modulates the repair of double-strand breaks by interacting with proteins like ATM kinase influencing cellular sensitivity to genotoxic stress. These pathways highlight its importance in maintaining cellular homeostasis and response to external stimuli.
- Associated diseases and disorders
KAP1 has known connections with cancer and neurological disorders due to its regulatory effects on gene expression and genome stability. In cancer aberrant KAP1 activity can lead to unregulated cell proliferation and survival often involving changes in its interaction with the p53 protein. In neurological disorders dysregulation of KAP1 is associated with impaired neural development and neurodegeneration. Altogether these associations highlight KAP1 as a potential target for therapeutic interventions in related diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Immunoprecipitation - Anti-KAP1 antibody (ab10484)
CTCF was immunoprecipitated from 1 mg HeLa whole cell lysate with ab10484 at 6 μg per reaction. Western blot was performed on the immunoprecipitate using ab10484 at 1 μg/mL.
Lysates prepared using NETN lysis buffer.
Lane 1: ab10484 IP in HeLa whole cell lysate.
Lane 2: Contol IgG in HeLa whole cell lysate.
Detection: Chemiluminescence.
Exposure time: 3 seconds.
All lanes: Immunoprecipitation - Anti-KAP1 antibody (ab10484)
Predicted band size: 88 kDa
Western blot - Anti-KAP1 antibody (ab10484)
Cells prepared using NETN lysis buffer. Chemiluminescence detection.
All lanes: Western blot - Anti-KAP1 antibody (ab10484) at 0.1 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: 293T whole cell lysate at 50 µg
Lane 3: 3T3 whole cell lysate at 50 µg
Predicted band size: 88 kDa
Exposure time: 1s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody (ab10484)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma labeling KAP1 with ab10484 at 1/5000 dilution (0.2 μg/ml). DAB detection, Hematoxylin counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody (ab10484)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse plasmacytoma, labeling KAP1 with ab10484 at 1 μg/mL. Detection: DAB.
Image courtesy of an anonymous customer review.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody (ab10484)
ab10484 staining KAP1 in murine coronal brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and permeabilized using 1% Triton. Samples were then blocked with 5% serum for 1 hour at 25°C followed by incubation with the primary antibody at 2μg/ml for 16 hours at 4°C. An Alexa-Fluor 488-conjugated donkey anti-rabbit polyclonal was used as secondary antibody at a 1/1000 dilution.
Dentate gyrus of the hippocampus immunostained with DAPI (top) and KAP1 (bottom), which nicely labels the nuclei of the entire dentate gyrus.
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