Anti-KAP1 (phospho S824) antibody

• ICC

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Immunocytochemistry - Anti-KAP1 (phospho S824) antibody (AB70369)

Detection of KAP1 (phospho S824) by ab70369 (1 : 200) in NBF-fixed HeLa cells by Immunocytochemistry. Cells were treated with etoposide (left) and untreated (right). Red fluorescent anti-rabbit IgG dylight 594 conjugated was used as a secondary antibody at 1/100 dilution.

• ICC

AbReview29889****

Immunocytochemistry - Anti-KAP1 (phospho S824) antibody (AB70369)

ab70369 staining KAP1 (phospho S824) (red) in Human U2OS cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were treated with 1 μM Etoposide for 3 hours prior to fixing. Cells were fixed with formaldehyde, permeabilized in 0.5% NP40 and blocked with 3% BSA for 2 hours at 21°C. Samples were incubated with primary antibody (1/1000 in PBS + 3% BSA) for 12 hours at 4°C. An Alexa Fluor®594-conjugated Mouse anti-rabbit IgG monoclonal (1/500) was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Ms. Can Zhou

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 (phospho S824) antibody (AB70369)

Detection of human Phospho KAP-1 (S824) using FFPE serial sections of asynchronous etoposide treated HeLa cells with MOCK CIP treatment (left) and etoposide treated HeLa cells with CIP treatment (right). Antibody : Affinity purified rabbit anti-Phospho KAP-1 (S824) ab70369 used at a dilution of 1 : 200 (1 µg/ml).

Detection : DAB

• IP

Supplier Data

Immunoprecipitation - Anti-KAP1 (phospho S824) antibody (AB70369)

Detection of Human KAP1 (phospho S824) by Western Blot of Immunoprecipitates.

Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HEK293T cells treated with 100 µM etoposide (+) or mock treated (-). Antibodies : Affinity purified rabbit anti-phospho KAP-1 (S824) antibody ab70369 and rabbit anti-KAP-1 recombinant monoclonal antibody ab243871 used for IP at 6 µg per reaction. For blotting immunoprecipitated Phospho KAP-1 (S824), ab70369 was used at 0.04 µg/ml.

Detection : Chemiluminescence with an exposure time of 1 second.

All lanes:

Immunoprecipitation - Anti-KAP1 (phospho S824) antibody (ab70369) at 0.04 µg/mL

Lane 1:

HEK293T cells treated with 100 µM etoposide (+)

Lane 2:

HEK293T cells mock treated (-)

Secondary

All lanes:

Goat anti-Rabbit Light Chain HRP Conjugate

Observed band size: 100-105 kDa

false

Exposure time: 1s

• WB

Supplier Data

Western blot - Anti-KAP1 (phospho S824) antibody (AB70369)

Whole cell lysate (10 µg) from HEK293T cells treated with 100 µM etoposide (+) or mock treated (-). ab70369 was used for WB at 0.04 µg/ml. Detection : Chemiluminescence with an exposure time of 30 seconds. Lower Panel : ab243871, Anti-KAP1 antibody [BL-248-2G6].

All lanes:

Western blot - Anti-KAP1 (phospho S824) antibody (ab70369) at 0.04 µg/mL

Lane 1:

HEK293T cells mock treated (-)

Lane 2:

HEK293T cells treated with 100 µM etoposide (+)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Observed band size: 100-105 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-KAP1 (phospho S824) antibody (AB70369)

Whole cell lysate (10 µg) from NIH 3T3 cells treated with 100 µM etoposide (+) or mock treated (-). ab70369 was used for WB at 0.04 µg/ml. Detection : Chemiluminescence with an exposure time of 30 seconds. Lower Panel : ab243871, Anti-KAP1 antibody [BL-248-2G6].

All lanes:

Western blot - Anti-KAP1 (phospho S824) antibody (ab70369) at 0.04 µg/mL

Lane 1:

NIH 3T3 cells mock treated (-)

Lane 2:

NIH 3T3 cells treated with 100 µM etoposide (+)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Observed band size: 100-105 kDa

false

Exposure time: 30s