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Rabbit Recombinant Monoclonal KAP1 phospho S824 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human TRIM28 phospho S824.

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Images

Western blot - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (AB272068), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (AB272068), expandable thumbnail
  • Immunoprecipitation - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (AB272068), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (AB272068), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (AB272068), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 8.2
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human TRIM28 phospho S824. The exact immunogen used to generate this antibody is proprietary information. Database link Q13263

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IF
Human
Tested
Tested
Expected
Tested
Mouse
Expected
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also a corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteasomal degradation; the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6. Mediates the nuclear localization of KOX1, ZNF268 and ZNF300 transcription factors. In association with isoform 2 of ZFP90, is required for the transcriptional repressor activity of FOXP3 and the suppressive function of regulatory T-cells (Treg) (PubMed:23543754). Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). In ESCs, in collaboration with SETDB1, is also required for H3K9me3 and silencing of endogenous and introduced retroviruses in a DNA-methylation independent-pathway (By similarity). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed:27029610). (Microbial infection) Plays a critical role in the shutdown of lytic gene expression during the early stage of herpes virus 8 primary infection. This inhibition is mediated through interaction with herpes virus 8 protein LANA1.

Alternative names

KAP1, RNF96, TIF1B, TRIM28, Transcription intermediary factor 1-beta, TIF1-beta, E3 SUMO-protein ligase TRIM28, KRAB-associated protein 1, KRAB-interacting protein 1, Nuclear corepressor KAP-1, RING finger protein 96, RING-type E3 ubiquitin transferase TIF1-beta, Tripartite motif-containing protein 28, KAP-1, KRIP-1

Recommended products

Rabbit Recombinant Monoclonal KAP1 phospho S824 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human TRIM28 phospho S824.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human TRIM28 phospho S824. The exact immunogen used to generate this antibody is proprietary information. Database link Q13263
Clone number
BL-246-7B5
Concentration
Loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

ab272068 is the BSA-free version of Anti-KAP1 (phospho S824) antibody [BL-246-7B5] ab243870.

This product is sold under License from Bethyl Laboratories, Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

KAP1 also known as TRIM28 or TIF1β is a multifunctional protein involved in transcriptional regulation. It has a molecular weight of approximately 87 kDa. This protein is expressed in a wide variety of human tissues where it plays a role as a co-repressor for KRAB domain-containing zinc finger proteins. KAP1 recruits various epigenetic modifiers to silence transcription and this activity depends on its phosphorylation status. You might explore KAP1 using KAP1 ELISA kits and study its post-translational modifications like phosphorylation in various conditions.

Biological function summary

KAP1 acts as a scaffold protein facilitating the assembly of large protein complexes that include chromatin remodelers and histone deacetylases. It is significant in maintaining chromatin structure and mediating gene silencing. KAP1 phosphorylation serves as a switch between its roles in transcriptional repression and activation. This protein also interacts with heterochromatin protein 1 (HP1) and other TRIM family proteins playing an important role in genomic stability by controlling gene expression and repair mechanisms.

Pathways

KAP1 participates actively in p53 and DNA damage response pathways. In the p53 pathway KAP1 regulates genes involved in the cell cycle and apoptosis acting in conjunction with the p53 protein. Within the DNA damage response KAP1 modulates the repair of double-strand breaks by interacting with proteins like ATM kinase influencing cellular sensitivity to genotoxic stress. These pathways highlight its importance in maintaining cellular homeostasis and response to external stimuli.

Associated diseases and disorders

KAP1 has known connections with cancer and neurological disorders due to its regulatory effects on gene expression and genome stability. In cancer aberrant KAP1 activity can lead to unregulated cell proliferation and survival often involving changes in its interaction with the p53 protein. In neurological disorders dysregulation of KAP1 is associated with impaired neural development and neurodegeneration. Altogether these associations highlight KAP1 as a potential target for therapeutic interventions in related diseases.

Product promise

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6 product images

  • Western blot - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (ab272068), expandable thumbnail

    Western blot - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (ab272068)

    All lanes: Western blot - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (ab272068) at 1/1000 dilution

    Lane 1: NIH/3T3 (Mouse embryonic fibroblast cell line) cells treated with 100 µM etoposide (+) at 50 µg

    Lane 2: NIH/3T3 (Mouse embryonic fibroblast cell line) cells, mock treated (-) at 50 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG antibody

    Predicted band size: 88 kDa

    Exposure time: 30s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (ab272068), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (ab272068)

    Formalin-fixed, paraffin-embedded human breast carcinoma tissue and serial sections of etoposide treated HeLa cells stained for KAPI (phospho S824) using Anti-KAP1 (phospho S824) antibody [BL-246-7B5] ab243870 at 1/100 dilution in immunohistochemical analysis. Mock phosphatase treated sections (left) and calf intestinal phosphatase-treated sections (right) are also shown. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-KAP1 (phospho S824) antibody [BL-246-7B5] ab243870).

  • Immunoprecipitation - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (ab272068), expandable thumbnail

    Immunoprecipitation - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (ab272068)

    KAP1 (phospho S824) was immunoprecipitated from 1 mg HEK-293T whole cell lysate treated with 100 μM etoposide or untreated, using Anti-KAP1 (phospho S824) antibody [BL-246-7B5] ab243870 at 20 μl per reaction. Western blot was performed on the immunoprecipitate using Anti-KAP1 (phospho S824) antibody [BL-246-7B5] ab243870 at 1/1000 dilution.

    Lane 1: Anti-KAP1 (phospho S824) antibody [BL-246-7B5] ab243870 IP in HEK-293T cell lysate treated with 100 μM etoposide.

    Lane 2: Anti-KAP1 (phospho S824) antibody [BL-246-7B5] ab243870 Ip in untreated HEK-293T whole cell lysate.

    Lane 3: Control IgG in HEK-293T whole cell lysate treated with 100 μM etoposide.

    Detection: Chemiluminescence with an exposure time of 10 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-KAP1 (phospho S824) antibody [BL-246-7B5] ab243870).

    All lanes: Immunoprecipitation - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] (Anti-KAP1 (phospho S824) antibody [BL-246-7B5] ab243870)

    Predicted band size: 88 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (ab272068), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (ab272068)

    Formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with etoposide (right) or untreated (left) labeling KAP-1 (phospho S824) (red) using Anti-Brd4 antibody [BL-149-2H5] ab243862 at 1/100 dilution in ICC analysis. DyLight® 594-conjugated goat anti-rabbit IgG was used as the secondary antibody. Counterstain: Phalloidin conjugated Alexa Fluor® 488 (green).

    This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-KAP1 (phospho S824) antibody [BL-246-7B5] ab243870).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (ab272068), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (ab272068)

    Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for KAPI (phospho S824) using Anti-KAP1 (phospho S824) antibody [BL-246-7B5] ab243870 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-KAP1 (phospho S824) antibody [BL-246-7B5] ab243870).

  • Western blot - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (ab272068), expandable thumbnail

    Western blot - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (ab272068)

    All lanes: Western blot - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (ab272068) at 1/1000 dilution

    Lane 1: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate, treated with 100 µM etoposide at 15 µg

    Lane 2: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate, mock treated at 15 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG antibody

    Predicted band size: 88 kDa

    Exposure time: 30s

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Product protocols

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