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Rabbit Recombinant Monoclonal KAP1 phospho S824 antibody. Suitable for IP, Dot, WB and reacts with Human, Mouse, Synthetic peptide samples. Cited in 10 publications.


Images

Western blot - Anti-KAP1 (phospho S824) antibody [EPR5248] (AB133440), expandable thumbnail
  • Immunoprecipitation - Anti-KAP1 (phospho S824) antibody [EPR5248] (AB133440), expandable thumbnail
  • Western blot - Anti-KAP1 (phospho S824) antibody [EPR5248] (AB133440), expandable thumbnail
  • Dot Blot - Anti-KAP1 (phospho S824) antibody [EPR5248] (AB133440), expandable thumbnail
  • Western blot - Anti-KAP1 (phospho S824) antibody [EPR5248] (AB133440), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPDotWB
Human
Tested
Expected
Tested
Mouse
Expected
Expected
Tested
Synthetic peptide
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
-
Notes

-

Expected
Expected

Species
Human, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Associated Products

Select an associated product type

14 products for Alternative Product

Target data

Function

Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also a corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteasomal degradation; the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6. Mediates the nuclear localization of KOX1, ZNF268 and ZNF300 transcription factors. In association with isoform 2 of ZFP90, is required for the transcriptional repressor activity of FOXP3 and the suppressive function of regulatory T-cells (Treg) (PubMed:23543754). Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). In ESCs, in collaboration with SETDB1, is also required for H3K9me3 and silencing of endogenous and introduced retroviruses in a DNA-methylation independent-pathway (By similarity). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed:27029610). (Microbial infection) Plays a critical role in the shutdown of lytic gene expression during the early stage of herpes virus 8 primary infection. This inhibition is mediated through interaction with herpes virus 8 protein LANA1.

Alternative names

KAP1, RNF96, TIF1B, TRIM28, Transcription intermediary factor 1-beta, TIF1-beta, E3 SUMO-protein ligase TRIM28, KRAB-associated protein 1, KRAB-interacting protein 1, Nuclear corepressor KAP-1, RING finger protein 96, RING-type E3 ubiquitin transferase TIF1-beta, Tripartite motif-containing protein 28, KAP-1, KRIP-1

Recommended products

Rabbit Recombinant Monoclonal KAP1 phospho S824 antibody. Suitable for IP, Dot, WB and reacts with Human, Mouse, Synthetic peptide samples. Cited in 10 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR5248
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

KAP1 also known as TRIM28 or TIF1β is a multifunctional protein involved in transcriptional regulation. It has a molecular weight of approximately 87 kDa. This protein is expressed in a wide variety of human tissues where it plays a role as a co-repressor for KRAB domain-containing zinc finger proteins. KAP1 recruits various epigenetic modifiers to silence transcription and this activity depends on its phosphorylation status. You might explore KAP1 using KAP1 ELISA kits and study its post-translational modifications like phosphorylation in various conditions.

Biological function summary

KAP1 acts as a scaffold protein facilitating the assembly of large protein complexes that include chromatin remodelers and histone deacetylases. It is significant in maintaining chromatin structure and mediating gene silencing. KAP1 phosphorylation serves as a switch between its roles in transcriptional repression and activation. This protein also interacts with heterochromatin protein 1 (HP1) and other TRIM family proteins playing an important role in genomic stability by controlling gene expression and repair mechanisms.

Pathways

KAP1 participates actively in p53 and DNA damage response pathways. In the p53 pathway KAP1 regulates genes involved in the cell cycle and apoptosis acting in conjunction with the p53 protein. Within the DNA damage response KAP1 modulates the repair of double-strand breaks by interacting with proteins like ATM kinase influencing cellular sensitivity to genotoxic stress. These pathways highlight its importance in maintaining cellular homeostasis and response to external stimuli.

Associated diseases and disorders

KAP1 has known connections with cancer and neurological disorders due to its regulatory effects on gene expression and genome stability. In cancer aberrant KAP1 activity can lead to unregulated cell proliferation and survival often involving changes in its interaction with the p53 protein. In neurological disorders dysregulation of KAP1 is associated with impaired neural development and neurodegeneration. Altogether these associations highlight KAP1 as a potential target for therapeutic interventions in related diseases.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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5 product images

  • Western blot - Anti-KAP1 (phospho S824) antibody [EPR5248] (ab133440), expandable thumbnail

    Western blot - Anti-KAP1 (phospho S824) antibody [EPR5248] (ab133440)

    All lanes: Western blot - Anti-KAP1 (phospho S824) antibody [EPR5248] (ab133440) at 1/1000 dilution

    Lane 1: Untreated NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate

    Lane 2: NIH/3T3 (Mouse embryonic fibroblast) treated with 3µM etoposide for 1 hour whole cell lysate

    Lane 3: NIH/3T3 (Mouse embryonic fibroblast) treated with 3µM etoposide for 1 hour whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 88 kDa

  • Immunoprecipitation - Anti-KAP1 (phospho S824) antibody [EPR5248] (ab133440), expandable thumbnail

    Immunoprecipitation - Anti-KAP1 (phospho S824) antibody [EPR5248] (ab133440)

    Purified ab133440 at 1/50 dilution (2μg) immunoprecipitating KAP1 in HeLa treated with 3uM etoposide for 1h whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) treated with 3uM etoposide for 1h whole cell lysate 10μg
    Lane 2 (+): ab133440 + HeLa treated with 3uM etoposide for 1h whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-p53 (phospho S6) antibody [Y179] ab32132 in HeLa treated with 3uM etoposide for 1h whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 110 kDa

    All lanes: Immunoprecipitation - Anti-KAP1 (phospho S824) antibody [EPR5248] (ab133440)

    Predicted band size: 88 kDa

  • Western blot - Anti-KAP1 (phospho S824) antibody [EPR5248] (ab133440), expandable thumbnail

    Western blot - Anti-KAP1 (phospho S824) antibody [EPR5248] (ab133440)

    Lane 1: Wild type HAP1 whole cell lysate (20 μg)
    Lane 2:     Wild type HAP1 + DMSO whole cell lysate (20 μg)
    Lane 3:     Wild type HAP1 + Bleomycin whole cell lysate (20 μg)
    Lane 4:     KAP1 knockout HAP1 whole cell lysate (20 μg)
    Lane 5:     KAP1 knockout HAP1 + DMSO whole cell lysate (20 μg)
    Lane 6:     KAP1 knockout HAP1 + Bleomycin whole cell lysate (20 μg)
    Lane 7:     HeLa + DMSO whole cell lysate (20 μg)
    Lane 8: HeLa + Bleomycin whole cell lysate (20 μg)

    Lanes 1 - 8: Merged signal (red and green). Green - ab133440 observed at 105 kDa. Red - loading control, Anti-Vinculin antibody [VIN-54] ab130007, observed at 125 kDa.

    ab133440 was shown to specifically react with KAP1 in wild type cells as signal was lost in KAP1 knockout cells. Wild-type and KAP1 knockout samples were subjected to SDS-PAGE. ab133440 and Anti-Vinculin antibody [VIN-54] ab130007 (Mouse anti-vinculin loading control) were incubated overnight at 4°C both at a 1/20000 dilution. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging. Treated with 30 μg/mL Bleomycin in DMSO for 30 minutes.

    All lanes: Western blot - Anti-KAP1 (phospho S824) antibody [EPR5248] (ab133440)

    Predicted band size: 88 kDa

  • Dot Blot - Anti-KAP1 (phospho S824) antibody [EPR5248] (ab133440), expandable thumbnail

    Dot Blot - Anti-KAP1 (phospho S824) antibody [EPR5248] (ab133440)

    Dot blot analysis of KAP1 (phospho S824) phospho peptide (Lane 1) and KAP1 non-phospho peptide (Lane 2) labelling KAP1 (phospho S824) with Unpurified ab133440 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as the secondary antibody at a dilution of 1/20,000. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.

  • Western blot - Anti-KAP1 (phospho S824) antibody [EPR5248] (ab133440), expandable thumbnail

    Western blot - Anti-KAP1 (phospho S824) antibody [EPR5248] (ab133440)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control.

    All lanes: Western blot - Anti-KAP1 (phospho S824) antibody [EPR5248] (ab133440) at 1/1000 dilution

    Lane 1: Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HeLa treated with 5µM etoposide for 8 hours whole cell lysate at 20 µg

    Lane 3: HeLa treated with 5µM etoposide for 8 hours whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 88 kDa

    Observed band size: 110 kDa

    Exposure time: 7s

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Product protocols

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