AB79558

Anti-Kappa light chain antibody [A8B5]

Name: Anti-Kappa light chain antibody [A8B5] (ab79558) | Abcam
Brand: Abcam Limited
SKU: AB79558

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(3 Publications)

Mouse Monoclonal Kappa light chain antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 3 publications.

1 Images

Flow Cytometry - Anti-Kappa light chain antibody [A8B5] (AB79558)

Flow Cyt

Unknown

Flow Cytometry - Anti-Kappa light chain antibody [A8B5] (AB79558)

ab79558 detecting Kappa light chain in B lymphocytes by Flow cytometry. B lymphocytes (CD19+) in a dot-plot Lambda PE vs. Kappa FITC. Kappa light chain detected by A8B5 antibody and lambda light chain by 4C2, CD19 by LT19 antibody (ab8331).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

A8B5

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-4 µg/mL", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Goat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1-4 µg/mL", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Guinea pig": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1-4 µg/mL", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Hamster": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1-4 µg/mL", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Rabbit": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1-4 µg/mL", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Sheep": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

ab79558 is purified from hybridoma culture supernatant by protein A affinity chromatography. Purity is > 95% (by SDS-PAGE).

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The kappa light chain alternatively known as Ig kappa chain is a component of immunoglobulins which are important for immune response. This protein has a molecular mass of roughly 25 kDa. It predominantly expresses in B lymphocytes where it pairs with heavy chains to form antibodies. Its expression is indicative of mature B cell functions serving as a marker for certain types of immune cells. The kappa light chain can also be detected outside of cells circulating in the blood and other bodily fluids.

Biological function summary

Kappa light chains are integral to the structure and function of antibodies. As part of the immunoglobulin molecule these chains contribute to antigen specificity by combining with heavy chains. Together they form the antigen-binding site. The kappa light chain is not part of a multiprotein complex; rather it directly participates in the antibody response. The presence of either kappa or lambda light chains can determine the particularities of antibody characteristics.

Pathways

Kappa light chains are elements of the immunological pathways notably the adaptive immune response. They interact with other proteins like immunoglobulin heavy chains to neutralize pathogens. The constant region of the kappa chain plays a role in determining the isotype of immunoglobulins which affects how antibodies mediate immune effector functions. Cross-linking of surface immunoglobulin by antigens can trigger B cell activation an essential step in the adaptive immune system.

Kappa light chains associate closely with multiple myeloma and certain types of lymphomas. Overproduction of kappa light chains in these diseases results in high levels detectable in the serum or urine which can be used diagnostically as markers. Also dysregulation of kappa chains correlates with autoimmune disorders whereby the immune system incorrectly targets its tissues. In disorders like these the interplay between kappa and lambda chains often arises showing imbalance between the two as a sign of abnormal B cell activity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Kappa light chain

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 83:4308-15 PubMed19224997

2009

Role of defective Oct-2 and OCA-B expression in immunoglobulin production and Kaposi's sarcoma-associated herpesvirus lytic reactivation in primary effusion lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel L Di Bartolo,Elizabeth Hyjek,Shannon Keller,Ilaria Guasparri,Hongyu Deng,Ren Sun,Amy Chadburn,Daniel M Knowles,Ethel Cesarman

Blood 111:4706-15 PubMed18296629

2008

IL-21 is expressed in Hodgkin lymphoma and activates STAT5: evidence that activated STAT5 is required for Hodgkin lymphomagenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ferenc A Scheeren,Sean A Diehl,Laura A Smit,Tim Beaumont,Marianne Naspetti,Richard J Bende,Bianca Blom,Kennosuke Karube,Koichi Ohshima,Carel J M van Noesel,Hergen Spits

Nature immunology 6:303-13 PubMed15711548

2005

STAT5 regulates the self-renewal capacity and differentiation of human memory B cells and controls Bcl-6 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Ferenc A Scheeren,Marianne Naspetti,Sean Diehl,Remko Schotte,Maho Nagasawa,Erwin Wijnands,Ramon Gimeno,Florry A Vyth-Dreese,Bianca Blom,Hergen Spits

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Kappa light chain antibody [A8B5]

Flow Cytometry - Anti-Kappa light chain antibody [A8B5] (AB79558)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise