Anti-Kappa light chain antibody [EPR5367-8] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [EPR5367-8] - BSA and Azide free (AB232152)

This data was developed using ab124727, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Kappa light chain with purified ab124727 at 1/5000 dilution (0.06 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• IP

Lab

Immunoprecipitation - Anti-Kappa light chain antibody [EPR5367-8] - BSA and Azide free (AB232152)

This data was developed using ab124727, the same antibody clone in a different buffer formulation.
Purified ab124727 at 1/50 dilution (2μg) immunoprecipitating Kappa light chain in Human spleen lysate.
Lane 1 (input) : Human spleen lysate 10μg
Lane 2 (+) : ab124727 + Human spleen lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab124727 in Human spleen lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 25 kDa

All lanes:

Immunoprecipitation - Anti-Kappa light chain antibody [EPR5367-8] (<a href='/en-us/products/primary-antibodies/kappa-light-chain-antibody-epr5367-8-ab124727'>ab124727</a>)

Predicted band size: 25 kDa

false

• ELISA

Lab

ELISA - Anti-Kappa light chain antibody [EPR5367-8] - BSA and Azide free (AB232152)

This data was developed using ab124727, the same antibody clone in a different buffer formulation.
ELISA analysis of Kappa Light Chains, Lambda Light Chains, Human IgA, Human IgM, Human IgG, Rat IgG,and Mouse IgG at 1000ng/mL using purified ab124727. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody at 1/2500 dilution.

• WB

Unknown

Western blot - Anti-Kappa light chain antibody [EPR5367-8] - BSA and Azide free (AB232152)

This data was developed using ab124727, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Kappa light chain antibody [EPR5367-8] (<a href='/en-us/products/primary-antibodies/kappa-light-chain-antibody-epr5367-8-ab124727'>ab124727</a>) at 1/5000 dilution

Lane 1:

Human tonsil lysate at 15 µg

Lane 2:

Human plasma lysate at 15 µg

Lane 3:

Human spleen lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 25 kDa

false