Mouse Recombinant Monoclonal KVD16 antibody. Carrier free. Suitable for IHC-P, Protein Array and reacts with Human, Recombinant full length protein - Human samples.
Images
![Protein Array - Anti-Kappa light chain antibody [rL1C1] - BSA and Azide free (AB237852), expandable thumbnail](https://content.abcam.com/products/images/kappa-light-chain-antibody-rl1c1-bsa-and-azide-free-ab237852--protein-array-img1998.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-Kappa light chain antibody [rL1C1] - BSA and Azide free (AB237852)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [rL1C1] - BSA and Azide free (AB237852), expandable thumbnail](https://content.abcam.com/products/images/kappa-light-chain-antibody-rl1c1-bsa-and-azide-free-ab237852--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img51032.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [rL1C1] - BSA and Azide free (AB237852)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link P01601
Reactivity data
Select an application| IHC-P | Protein Array | |
|---|---|---|
| Human | Tested | Expected |
| Rat | Not recommended | Not recommended |
| Recombinant full length protein - Human | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-1 µg/mL | Notes Incubate with primary antibody for 30 minutes at RT Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Incubate with primary antibody for 30 minutes at RT Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
V region of the variable domain of immunoglobulin light chains that participates in the antigen recognition (PubMed:24600447). Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).
Alternative names
Immunoglobulin kappa variable 1D-16, Ig kappa chain V-I region HK146, Ig kappa chain V-I region HK189, IGKV1D-16
Recommended products
Mouse Recombinant Monoclonal KVD16 antibody. Carrier free. Suitable for IHC-P, Protein Array and reacts with Human, Recombinant full length protein - Human samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link P01601
- Carrier free
- Yes
- Clone number
- rL1C1
- Purification technique
- Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from Bioreactor Concentrate by Protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The kappa light chain alternatively known as Ig kappa chain is a component of immunoglobulins which are important for immune response. This protein has a molecular mass of roughly 25 kDa. It predominantly expresses in B lymphocytes where it pairs with heavy chains to form antibodies. Its expression is indicative of mature B cell functions serving as a marker for certain types of immune cells. The kappa light chain can also be detected outside of cells circulating in the blood and other bodily fluids.
- Biological function summary
Kappa light chains are integral to the structure and function of antibodies. As part of the immunoglobulin molecule these chains contribute to antigen specificity by combining with heavy chains. Together they form the antigen-binding site. The kappa light chain is not part of a multiprotein complex; rather it directly participates in the antibody response. The presence of either kappa or lambda light chains can determine the particularities of antibody characteristics.
- Pathways
Kappa light chains are elements of the immunological pathways notably the adaptive immune response. They interact with other proteins like immunoglobulin heavy chains to neutralize pathogens. The constant region of the kappa chain plays a role in determining the isotype of immunoglobulins which affects how antibodies mediate immune effector functions. Cross-linking of surface immunoglobulin by antigens can trigger B cell activation an essential step in the adaptive immune system.
- Associated diseases and disorders
Kappa light chains associate closely with multiple myeloma and certain types of lymphomas. Overproduction of kappa light chains in these diseases results in high levels detectable in the serum or urine which can be used diagnostically as markers. Also dysregulation of kappa chains correlates with autoimmune disorders whereby the immune system incorrectly targets its tissues. In disorders like these the interplay between kappa and lambda chains often arises showing imbalance between the two as a sign of abnormal B cell activity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Protein Array - Anti-Kappa light chain antibody [rL1C1] - BSA and Azide free (ab237852), expandable thumbnail](https://content.abcam.com/products/images/kappa-light-chain-antibody-rl1c1-bsa-and-azide-free-ab237852--protein-array-img1998.jpg "Protein Array - Anti-Kappa light chain antibody [rL1C1] - BSA and Azide free (ab237852)")
Protein Array - Anti-Kappa light chain antibody [rL1C1] - BSA and Azide free (ab237852)
This data was produced with Anti-Kappa light chain antibody [rL1C1] ab238008, the same antibody in a different formulation with BSA and Azide.
Anti-Kappa light chain antibody [rL1C1] ab238008 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [rL1C1] - BSA and Azide free (ab237852), expandable thumbnail](https://content.abcam.com/products/images/kappa-light-chain-antibody-rl1c1-bsa-and-azide-free-ab237852--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img51032.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [rL1C1] - BSA and Azide free (ab237852)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [rL1C1] - BSA and Azide free (ab237852)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for Kappa light chain using ab237852 at 1 μg/ml in immunohistochemical analysis.
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