Anti-Kappa light chain antibody [RM103]

• ELISA

Supplier Data

ELISA - Anti-Kappa light chain antibody [RM103] (AB190484)

ELISA of mouse immunoglobulins shows ab190484 reacted to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab190484 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.

• ELISA

Supplier Data

ELISA - Anti-Kappa light chain antibody [RM103] (AB190484)

A titer ELISA of mouse IgG1 kappa. The plate was coated with different amounts of mouse IgG1 kappa. A serial dilution of ab190484 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.

• WB

Supplier Data

Western blot - Anti-Kappa light chain antibody [RM103] (AB190484)

ab190484 reacted to Non-reduced IgG1 kappa (~150 kDa), and slightly reacted to reduced kappa light chain (~25 kDa).

All lanes:

Western blot - Anti-Kappa light chain antibody [RM103] (ab190484) at 0.2 µg/mL

Lane 1:

Non-reduced Mouse IgG1 kappa at 0.02 µg

Lane 2:

Reduced Mouse IgG1 kappa at 0.02 µg

Lane 3:

Non-reduced Mouse IgG1 lambda at 0.02 µg

Lane 4:

Reduced Mouse IgG1 lambda at 0.02 µg

Predicted band size: 25 kDa

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