Rabbit Recombinant Monoclonal Kappa Opioid Receptor antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat samples. Cited in 11 publications.
Images
![Western blot - Anti-Kappa Opioid Receptor antibody [EPR18881] (AB183825), expandable thumbnail](https://content.abcam.com/products/images/kappa-opioid-receptor-antibody-epr18881-ab183825--western-blot-img9687.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Kappa Opioid Receptor antibody [EPR18881] (AB183825)")
![Western blot - Anti-Kappa Opioid Receptor antibody [EPR18881] (AB183825), expandable thumbnail](https://content.abcam.com/products/images/kappa-opioid-receptor-antibody-epr18881-ab183825--western-blot-img34936.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Kappa Opioid Receptor antibody [EPR18881] (AB183825)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (AB183825), expandable thumbnail](https://content.abcam.com/products/images/kappa-opioid-receptor-antibody-epr18881-ab183825--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img45851.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (AB183825)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (AB183825), expandable thumbnail](https://content.abcam.com/products/images/kappa-opioid-receptor-antibody-epr18881-ab183825--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img86438.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (AB183825)")
![Immunoprecipitation - Anti-Kappa Opioid Receptor antibody [EPR18881] (AB183825), expandable thumbnail](https://content.abcam.com/products/images/kappa-opioid-receptor-antibody-epr18881-ab183825--immunoprecipitation-img24889.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Kappa Opioid Receptor antibody [EPR18881] (AB183825)")
Publications
Filter by
- Evidence-based complementary and alternative medicine : eCAM 2023:60589512023Angelica Yinzi Alleviates Pruritus-Related Atopic Dermatitis through Skin Repair, Antioxidation, and Balancing Peripheral - and -opioid Receptors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Liu,Yang Luo,Wanci Song,Hanxiong Dan,Li Li,Daonian Zhou,Pengtao YouPubMed 37790739
- The Journal of clinical investigation 133:2023Nucleus accumbens D1/D2 circuits control opioid withdrawal symptoms in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYongsheng Zhu,Kejia Wang,Tengfei Ma,Yuanyuan Ji,Yin Lou,Xiaoyu Fu,Ye Lu,Yige Liu,Wei Dang,Qian Zhang,Fangyuan Yin,Kena Wang,Bing Yu,Hongbo Zhang,Jianghua Lai,Yunpeng WangPubMed 37561576
- Current research in neurobiology 5:1001072023Δ9-Tetrahydrocannabinol does not upregulate an aversive dopamine receptor mechanism in adolescent brain unlike in adults.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarie-Eve Di Raddo,Marija Milenkovic,Meenalochani Sivasubramanian,Ahmed Hasbi,Jack Bergman,Sarah Withey,Bertha K Madras,Susan R GeorgePubMed 38020805
- iScience 26:1075362023Kappa opioid receptor in nucleus accumbens regulates depressive-like behaviors following prolonged morphine withdrawal in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinyu Zhang,Ye Lu,Min Jia,Yuying Bai,Lulu Sun,Ziqing Dong,Wenrong Tian,Fangyuan Yin,Shuguang Wei,Yunpeng WangPubMed 37636073
- BMC neuroscience 23:582022Morphine-induced changes in the function of microglia and macrophages after acute spinal cord injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMabel N Terminel,Carla Bassil,Josephina Rau,Amanda Trevino,Cristina Ruiz,Robert Alaniz,Michelle A HookPubMed 36217122
- Frontiers in cellular neuroscience 16:8948862022The Basolateral Amygdala to Ventral Hippocampus Circuit Controls Anxiety-Like Behaviors Induced by Morphine Withdrawal.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCuola Deji,Peng Yan,Yuanyuan Ji,Xinyue Yan,Yue Feng,Jincen Liu,Yige Liu,Shuguang Wei,Yongsheng Zhu,Jianghua LaiPubMed 35726232
- Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 17:350-3662021Intranasal Salvinorin A Improves Long-term Neurological Function via Immunomodulation in a Mouse Ischemic Stroke Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDilidaer Misilimu,Wei Li,Di Chen,Pengju Wei,Yichen Huang,Sicheng Li,John Grothusen,Yanqin GaoPubMed 34596819
- Theranostics 11:2822-28442021Roles of CatSper channels in the pathogenesis of asthenozoospermia and the therapeutic effects of acupuncture-like treatment on asthenozoospermia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZi-Run Jin et. al.PubMed 33456575
- Frontiers in neuroscience 13:13922020Escalated Oxycodone Self-Administration and Punishment: Differential Expression of Opioid Receptors and Immediate Early Genes in the Rat Dorsal Striatum and Prefrontal Cortex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristopher A Blackwood,Michael T McCoy,Bruce Ladenheim,Jean Lud CadetPubMed 31998063
- International immunopharmacology 73:23-402019Methionine enkephalin (MENK) regulates the immune pathogenesis of type 2 diabetes mellitus via the IL-33/ST2 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKeying Zhang et. al.PubMed 31078923
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Mouse | Tested | Tested | Tested |
| Rat | Expected | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
G-protein coupled opioid receptor that functions as a receptor for endogenous alpha-neoendorphins and dynorphins, but has low affinity for beta-endorphins. Also functions as a receptor for various synthetic opioids and for the psychoactive diterpene salvinorin A. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain. Plays a role in mediating reduced physical activity upon treatment with synthetic opioids. Plays a role in the regulation of salivation in response to synthetic opioids. May play a role in arousal and regulation of autonomic and neuroendocrine functions.
Alternative names
Kappa-type opioid receptor, K-OR-1, KOR-1, MSL-1, Oprk1
Recommended products
Rabbit Recombinant Monoclonal Kappa Opioid Receptor antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat samples. Cited in 11 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR18881
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Kappa Opioid Receptor also known as KOR or kappa receptors is a type of opioid receptor that plays an important role in the nervous system. It is a protein with an approximate mass of 43 kDa. This receptor mainly expresses in the human brain especially in regions involved in pain and stress responses such as the hypothalamus and thalamus. Kappa receptors are involved in modulating neurotransmitter release and can affect synaptic transmission.
- Biological function summary
Kappa opioid receptors mediate pain perception mood regulation and stress response. They form part of a larger receptor family the G-protein coupled receptors (GPCRs) which are critical in cellular signaling. Activation of these receptors can produce analgesic effects differing from other opioid receptors due to their unique mechanism of action. Kappa receptors interact with endogenous ligands called dynorphins which are peptide neurotransmitters involved in natural pain relief processes.
- Pathways
Kappa opioid receptors are integral to the dopaminergic and serotonergic pathways. In the dopaminergic pathway kappa receptors reduce dopamine release influencing the reward system and potentially affecting addiction behaviors. In the serotonergic pathway they modulate serotonin levels connecting to mood and anxiety regulation. These pathways also involve proteins like the mu-opioid receptor and delta-opioid receptor which along with kappa receptors form a complex regulatory network.
- Associated diseases and disorders
Kappa opioid receptors have been linked to depression and drug addiction. In depression dysfunctional kappa receptor activity may disrupt mood regulation contributing to depressive symptoms. The associated protein dynorphin plays a role in this condition by interacting with kappa receptors. In drug addiction these receptors influence the brain's reward circuits impacting addiction severity through altered dopamine signaling. Understanding kappa receptors can offer insights into new therapeutic approaches for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825), expandable thumbnail](https://content.abcam.com/products/images/kappa-opioid-receptor-antibody-epr18881-ab183825--western-blot-img9687.jpg "Western blot - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)")
Western blot - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID:20574683).
All lanes: Western blot - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825) at 1/1000 dilution
Lane 1: Mouse brain lysate at 10 µg
Lane 2: Mouse heart lysate at 10 µg
Lane 3: Mouse kidney lysate at 10 µg
Lane 4: Mouse spleen lysate at 10 µg
Lane 5: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg
Lane 6: Neuro-2a (Mouse neuroblastoma cells) whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 42 kDa
Observed band size: 58 kDa
Exposure time: 3min
![Western blot - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825), expandable thumbnail](https://content.abcam.com/products/images/kappa-opioid-receptor-antibody-epr18881-ab183825--western-blot-img34936.jpg "Western blot - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)")
Western blot - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID:20574683).
All lanes: Western blot - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825) at 1/1000 dilution
Lane 1: Rat brain lysate at 10 µg
Lane 2: Rat heart lysate at 10 µg
Lane 3: Rat kidney lysate at 10 µg
Lane 4: Rat spleen lysate at 10 µg
Lane 5: C6 (Rat glial tumor cells) whole cell lysate at 10 µg
Lane 6: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 42 kDa
Observed band size: 58 kDa
Exposure time: 30s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825), expandable thumbnail](https://content.abcam.com/products/images/kappa-opioid-receptor-antibody-epr18881-ab183825--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img45851.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling Kappa Opioid Receptor with ab183825 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Mainly membrane staining on neuron cell of mouse hippocampus is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825), expandable thumbnail](https://content.abcam.com/products/images/kappa-opioid-receptor-antibody-epr18881-ab183825--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img86438.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Kappa Opioid Receptor with ab183825 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Negative staining on mouse spleen.
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825), expandable thumbnail](https://content.abcam.com/products/images/kappa-opioid-receptor-antibody-epr18881-ab183825--immunoprecipitation-img24889.jpg "Immunoprecipitation - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)")
Immunoprecipitation - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
Kappa Opioid Receptor was immunoprecipitated from 1mg of Mouse brain whole cell lysate with ab183825 at 1/100 dilution.
Western blot was performed from the immunoprecipitate using ab183825 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.
Lane 1: Mouse brain whole cell lysate 10μg (Input).
Lane 2: ab183825 IP in Mouse brain whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab183825 in Mouse brain whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
All lanes: Immunoprecipitation - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
Predicted band size: 42 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825), expandable thumbnail](https://content.abcam.com/products/images/kappa-opioid-receptor-antibody-epr18881-ab183825--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img122303.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labeling Kappa Opioid Receptor with ab183825 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Mainly membrane staining on neuron cells of rat hippocampus is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825), expandable thumbnail](https://content.abcam.com/products/images/kappa-opioid-receptor-antibody-epr18881-ab183825--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img17924.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Kappa Opioid Receptor with ab183825 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Negative staining on rat spleen.
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825), expandable thumbnail](https://content.abcam.com/products/images/kappa-opioid-receptor-antibody-epr18881-ab183825--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img122303.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825), expandable thumbnail](https://content.abcam.com/products/images/kappa-opioid-receptor-antibody-epr18881-ab183825--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img17924.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com