JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB272641

Anti-Kaptin antibody

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Polyclonal Kaptin antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human KICSTOR complex protein kaptin aa 150-250.

View Alternative Names

KICSTOR complex protein kaptin, Actin-associated protein 2E4, KPTN

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kaptin antibody (AB272641)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kaptin antibody (AB272641)

Paraffin-embedded formalin-fixed Human colon tissue stained for Kaptin using ab272641 at 1/200 in immunohistochemical analysis.

Western blot - Anti-Kaptin antibody (AB272641)
  • WB

Unknown

Western blot - Anti-Kaptin antibody (AB272641)

Lane 1:

Western blot - Anti-Kaptin antibody (ab272641)

Lane 2:

Western blot - Anti-Kaptin antibody (ab272641) at 0.4 µg/mL

Lane 1:

Molecular weight ladder

Lane 2:

RT4 (Human urinary bladder cancer cell line) cell lysate

Predicted band size: 48 kDa

false

Western blot - Anti-Kaptin antibody (AB272641)
  • WB

Unknown

Western blot - Anti-Kaptin antibody (AB272641)

All lanes:

Western blot - Anti-Kaptin antibody (ab272641) at 0.4 µg/mL

Lane 1:

NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

Lane 2:

NBT-II (Rat bladder carcinoma cell line) cell lysate

Predicted band size: 48 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human KICSTOR complex protein kaptin aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

Q9Y664

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kaptin also known as KPTN is an actin-binding protein with a molecular weight of approximately 45 kDa. It plays an important mechanical role in the regulation of actin cytoskeleton dynamics. Kaptin localizes to cellular structures where actin remodeling occurs such as the tips of filopodia and lamellipodia. It expresses widely but shows increased presence in neuronal tissues where active cytoskeletal rearrangements take place.
Biological function summary

Kaptin acts in cellular processes requiring actin filament regulation. It is a part of the complex interacting with actin influencing cellular movement shape and signal transduction. By binding actin Kaptin promotes changes in cell motility and is particularly important in neuronal cells where rapid restructuring is needed for functions like synaptic signaling and plasticity.

Pathways

Kaptin plays a role in the regulation of the actin cytoskeleton pathway which is critical for cell movement and signaling. It interacts with proteins such as profilin and gelsolin which also modulate actin dynamics. Its involvement is vital in pathways facilitating cell motility and growth cone guidance in neurons showing its importance in maintaining cellular architecture and communication.

Scientists relate Kaptin to neurological conditions and sensory impairments. Mutations in the KPTN gene associate with conditions like intellectual disability and cortical malformations. Kaptin's connectivity with actin and related proteins such as cofilin highlights its potential impact on disease when dysregulated influencing neuronal development and function.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

As part of the KICSTOR complex functions in the amino acid-sensing branch of the TORC1 signaling pathway. Recruits, in an amino acid-independent manner, the GATOR1 complex to the lysosomal membranes and allows its interaction with GATOR2 and the RAG GTPases. Functions upstream of the RAG GTPases and is required to negatively regulate mTORC1 signaling in absence of amino acids. In absence of the KICSTOR complex mTORC1 is constitutively localized to the lysosome and activated. The KICSTOR complex is also probably involved in the regulation of mTORC1 by glucose.
See full target information KICSTOR complex protein kaptin
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB272649

Anti-NHE-7 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NHE-7 antibody (AB272649)

  • 0
  • 0
Primary Antibodies

AB272666

Anti-PINCH-2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-PINCH-2 antibody (AB272666)

  • 0
  • 0
Primary Antibodies

AB272635

Anti-MOSPD3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-MOSPD3 antibody (AB272635)

  • 0
  • 0
Primary Antibodies

AB272668

Anti-HSD17B6 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B6 antibody (AB272668)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com