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AB236990

Anti-KAR antibody

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(1 Publication)

Rabbit Polyclonal KAR antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HSD17B12 aa 1-200.

View Alternative Names

SDR12C1, HSD17B12, Very-long-chain 3-oxoacyl-CoA reductase, 17-beta-hydroxysteroid dehydrogenase 12, 3-ketoacyl-CoA reductase, Estradiol 17-beta-dehydrogenase 12, Short chain dehydrogenase/reductase family 12C member 1, 17-beta-HSD 12, KAR

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAR antibody (AB236990)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAR antibody (AB236990)

Paraffin-embedded human breast cancer tissue stained for KAR using ab236990 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-KAR antibody (AB236990)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KAR antibody (AB236990)

U-251 MG (human brain glioma cell line) cells labeling KAR using ab236990 (green) at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAR antibody (AB236990)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAR antibody (AB236990)

Paraffin-embedded human small intestine tissue stained for KAR using ab236990 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-KAR antibody (AB236990)
  • WB

Supplier Data

Western blot - Anti-KAR antibody (AB236990)

All lanes:

Western blot - Anti-KAR antibody (ab236990) at 1/500 dilution

Lane 1:

U-251 MG (human brain glioma cell line) whole cell lysate

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 34 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human HSD17B12 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q53GQ0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kainate receptors (KARs) are ionotropic receptors important for fast excitatory neurotransmission. They are also known as 'kainate-type glutamate receptors' and are part of the larger glutamate receptor family. The typical mass of these receptors can vary due to different subunits but they usually range between 100 and 120 kDa. KARs express in the central nervous system especially in regions such as the hippocampus and cortex. They involve in synaptic transmission and plasticity through their role as ligand-gated ion channels.
Biological function summary

KARs participate in mediating excitatory synaptic transmission and are part of a complex with other ion channels and receptors like AMPA and NMDA receptors. They regulate synaptic strength and plasticity which are important for learning and memory processes. Active in both pre- and postsynaptic sites KARs can either enhance or inhibit neurotransmitter release affecting neuronal excitability. Their ability to modulate other neurotransmitter systems highlights their diverse influence on neural circuits.

Pathways

KARs are critical components in the glutamatergic signaling pathway which includes AMPA and NMDA receptors as well. They work closely with these proteins to regulate synaptic plasticity and excitatory neurotransmission. Investigations show the involvement of KARs in the long-term potentiation pathway important for memory encoding. Additionally they overlap with metabotropic glutamate receptors influencing intracellular signaling cascades and calcium dynamics.

KARs have associations with epilepsy and neurodegenerative diseases like Alzheimer's disease. Dysfunctional KAR signaling can lead to altered neuronal excitability contributing to epilepsy pathogenesis. Moreover in Alzheimer's altered glutamatergic transmission due to impaired KAR function associates with memory deficits. Their connection with AMPA and NMDA receptors is significant here as these proteins also pertain to synaptic dysfunction and cognitive impairments observed in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the second of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme has a 3-ketoacyl-CoA reductase activity, reducing 3-ketoacyl-CoA to 3-hydroxyacyl-CoA, within each cycle of fatty acid elongation. Thereby, it may participate in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. May also catalyze the transformation of estrone (E1) into estradiol (E2) and play a role in estrogen formation.
See full target information HSD17B12
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 42:e111268 PubMed36408830

2022

Stearate-derived very long-chain fatty acids are indispensable to tumor growth.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaoyun Chu,Ping Liu,Yihan Song,Ronghui Yang,Jing An,Xuewei Zhai,Jing Niu,Chuanzhen Yang,Binghui Li
View all publications
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