AB251166

Anti-KAT1 / HAT1 antibody [EPR18661] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal KAT1 / HAT1 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 1 publication.

View Alternative Names

KAT1, HAT1, Histone acetyltransferase type B catalytic subunit, Histone acetyltransferase 1, Histone methacryltransferase HAT1

10 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT1 / HAT1 antibody [EPR18661] - BSA and Azide free (AB251166)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT1 / HAT1 antibody [EPR18661] - BSA and Azide free (AB251166)

This data was developed using ab193097, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling KAT1 / HAT1 with ab193097 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on human tonsil is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-KAT1 / HAT1 antibody [EPR18661] - BSA and Azide free (AB251166)

This data was developed using ab193097, the same antibody clone in a different buffer formulation.

KAT1 / HAT1 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab193097 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab193097 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HeLa whole cell lysate 10ug (Input).
Lane 2 : ab193097 IP in HeLa whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab193097 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-KAT1 / HAT1 antibody [EPR18661] (<a href='/en-us/products/primary-antibodies/kat1-hat1-antibody-epr18661-ab193097'>ab193097</a>)

Predicted band size: 49 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT1 / HAT1 antibody [EPR18661] - BSA and Azide free (AB251166)

This data was developed using ab193097, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling KAT1 / HAT1 with ab193097 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on mouse colon is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT1 / HAT1 antibody [EPR18661] - BSA and Azide free (AB251166)

This data was developed using ab193097, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling KAT1 / HAT1 with ab193097 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on rat colon is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-KAT1 / HAT1 antibody [EPR18661] - BSA and Azide free (AB251166)

This data was developed using ab193097, the same antibody clone in a different buffer formulation.

KAT1 / HAT1 was immunoprecipitated from 1mg of F9 (Mouse embryonic carcinoma cell line) whole cell lysate with ab193097 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab193097 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : F9 whole cell lysate 10ug (Input).
Lane 2 : ab193097 IP in F9 whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab193097 in F9 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-KAT1 / HAT1 antibody [EPR18661] (<a href='/en-us/products/primary-antibodies/kat1-hat1-antibody-epr18661-ab193097'>ab193097</a>)

Predicted band size: 49 kDa

false

Supplier Data

Western blot - Anti-KAT1 / HAT1 antibody [EPR18661] - BSA and Azide free (AB251166)

This data was developed using ab193097, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KAT1 / HAT1 antibody [EPR18661] (<a href='/en-us/products/primary-antibodies/kat1-hat1-antibody-epr18661-ab193097'>ab193097</a>) at 1/2000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 3:

F9 (Mouse embryonic carcinoma cell line) whole cell lysate at 20 µg

Lane 4:

LLC (Mouse lung carcinoma) whole cell lysate at 20 µg

Lane 5:

Mouse thymus lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 49 kDa

Observed band size: 45 kDa

false

Exposure time: 8s

Lab

Western blot - Anti-KAT1 / HAT1 antibody [EPR18661] - BSA and Azide free (AB251166)

This data was developed using ab193097, the same antibody clone in a different buffer formulation.

Lane 1 : Wild-type HAP1 cell lysate (20 μg)

Lane 2 : AT1 / HAT1 knockout HAP1 cell lysate (20 μg)

Lane 3 : HeLa cell lysate (20 μg)

Lane 4 : NIH3T3 cell lysate (20 μg)

Lanes 1 - 4 : erged signal (red and green). Green - ab193097 observed at 48 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab193097 was shown to specifically react with KAT1 / HAT1 when KAT1 / HAT1 knockout samples were used. Wild-type and KAT1 / HAT1 knockout samples were subjected to SDS-PAGE. ab193097 and ab8245 (loading control to GAPDH) were diluted to 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-KAT1 / HAT1 antibody [EPR18661] (<a href='/en-us/products/primary-antibodies/kat1-hat1-antibody-epr18661-ab193097'>ab193097</a>)

Predicted band size: 49 kDa

false

Supplier Data

Western blot - Anti-KAT1 / HAT1 antibody [EPR18661] - BSA and Azide free (AB251166)

This data was developed using ab193097, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KAT1 / HAT1 antibody [EPR18661] (<a href='/en-us/products/primary-antibodies/kat1-hat1-antibody-epr18661-ab193097'>ab193097</a>) at 1/2000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Lane 3:

Human fetal kidney lysate at 10 µg

Lane 4:

Human fetal spleen lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 49 kDa

Observed band size: 45 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-KAT1 / HAT1 antibody [EPR18661] - BSA and Azide free (AB251166)

This data was developed using ab193097, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KAT1 / HAT1 antibody [EPR18661] (<a href='/en-us/products/primary-antibodies/kat1-hat1-antibody-epr18661-ab193097'>ab193097</a>) at 1/2000 dilution

Lane 1:

C6 (Rat glial tumor cells) whole cell lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Lane 4:

NIH/3T3 (Mouse embryonic fibroblast cells) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 49 kDa

Observed band size: 45 kDa

false

Exposure time: 5s

Unknown

Western blot - Anti-KAT1 / HAT1 antibody [EPR18661] - BSA and Azide free (AB251166)

This data was developed using ab193097, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KAT1 / HAT1 antibody [EPR18661] (<a href='/en-us/products/primary-antibodies/kat1-hat1-antibody-epr18661-ab193097'>ab193097</a>)

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse heart lysate at 10 µg

Lane 3:

Mouse kidney lysate at 10 µg

Lane 4:

Rat brain lysate at 10 µg

Lane 5:

Rat heart lysate at 10 µg

Lane 6:

Rat kidney lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 49 kDa

Observed band size: 45 kDa

false

Exposure time: 3min

Unconjugated

Anti-KAT1 / HAT1 antibody [EPR18661]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18661

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab251166 is the carrier-free version of ab193097.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein KAT1 also known as HAT1 (Histone Acetyltransferase 1) functions as an enzyme responsible for acetylating newly synthesized histone proteins. It has a mass of approximately 46 kDa. KAT1 is mostly expressed in the nucleus of cells where it modifies histones by adding acetyl groups to the N-terminal lysines a process essential for chromatin modification. The enzyme is important for facilitating transcriptional activation by altering chromatin structure and is widely observed in various eukaryotic organisms.

Biological function summary

KAT1 acts as a part of the histone acetyltransferase complex which plays a major role in regulating gene expression. By acetylating histone proteins it reduces chromatin compaction and allows gene transcription machinery access to DNA. This alteration can significantly influence cellular processes such as cell cycle regulation and DNA repair. As KAT1 interacts with several protein partners including other acetylation enzymes it serves as an important player in managing chromatin dynamics and maintaining genomic integrity.

Pathways

The role of KAT1 extends to involvement in the cell cycle and DNA damage repair pathways. In the cell cycle pathway KAT1 facilitates transcription required for progression through various phases of the cycle. In DNA damage response pathways KAT1 contributes to repairing DNA lesions by modifying chromatin to allow repair machinery access. It interacts with proteins such as p300 and CBP which are also acetyltransferases to modulate these pathways effectively and ensure cellular responses to DNA insults are adequate.

KAT1's function connects with cancer and neurodegenerative diseases due to its role in gene regulation and DNA repair. In cancer aberrant acetylation by KAT1 can lead to unregulated cell division and tumor formation while in neurodegenerative disorders impaired KAT1 activity might contribute to neuronal damage and death due to faulty DNA repair. The protein interacts with p53 another key player in both pathways impacting disease progression by modulating tumor suppressor functions and ensuring cellular stability under stress.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Histone acetyltransferase that plays a role in different biological processes including cell cycle progression, glucose metabolism, histone production or DNA damage repair (PubMed : 20953179, PubMed : 23653357, PubMed : 31278053, PubMed : 32081014). Coordinates histone production and acetylation via H4 promoter binding (PubMed : 31278053). Acetylates histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, histone H2A at 'Lys-5' (H2AK5ac) (PubMed : 11585814, PubMed : 22615379). Drives H4 production by chromatin binding to support chromatin replication and acetylation. Since transcription of H4 genes is tightly coupled to S-phase, plays an important role in S-phase entry and progression (PubMed : 31278053). Promotes homologous recombination in DNA repair by facilitating histone turnover and incorporation of acetylated H3.3 at sites of double-strand breaks (PubMed : 23653357). In addition, acetylates other substrates such as chromatin-related proteins (PubMed : 32081014). Also acetylates RSAD2 which mediates the interaction of ubiquitin ligase UBE4A with RSAD2 leading to RSAD2 ubiquitination and subsequent degradation (PubMed : 31812350). In addition to protein acetyltransferase, can use different acyl-CoA substrates, such as 2-methylpropenoyl-CoA (methacryl-CoA), and is able to mediate histone methacrylation (PubMed : 34961760).. (Microbial infection) Contributes to hepatitis B virus (HBV) replication by acetylating histone H4 at the sites of 'Lys-5' and 'Lys-12' on the covalently closed circular DNA (cccDNA) minichromosome leading to its accumulation within the host cell.

See full target information HAT1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 21:4027-4050 PubMed40612676

2025

Exogenous SPD inhibits trastuzumab-mediated cardiomyocyte pyroptosis through SIRT3-regulated mitochondrial quality control.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Yu,Yan Yang,Tianzuo Chen,Qianbing Wang,Zitong Wang,Xi Gao,Qianxue Wang,Jinxiang Guo,Yuqin Wang,Yajie Zhao,Shilin Wang,Wei Lu,Xing Luo,Tielei Gao,Jiayuan Kou,Hong Li,Liming Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KAT1 / HAT1 antibody [EPR18661] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT1 / HAT1 antibody [EPR18661] - BSA and Azide free (AB251166)

Immunoprecipitation - Anti-KAT1 / HAT1 antibody [EPR18661] - BSA and Azide free (AB251166)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT1 / HAT1 antibody [EPR18661] - BSA and Azide free (AB251166)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT1 / HAT1 antibody [EPR18661] - BSA and Azide free (AB251166)

Immunoprecipitation - Anti-KAT1 / HAT1 antibody [EPR18661] - BSA and Azide free (AB251166)

Western blot - Anti-KAT1 / HAT1 antibody [EPR18661] - BSA and Azide free (AB251166)

Western blot - Anti-KAT1 / HAT1 antibody [EPR18661] - BSA and Azide free (AB251166)

Western blot - Anti-KAT1 / HAT1 antibody [EPR18661] - BSA and Azide free (AB251166)

Western blot - Anti-KAT1 / HAT1 antibody [EPR18661] - BSA and Azide free (AB251166)

Western blot - Anti-KAT1 / HAT1 antibody [EPR18661] - BSA and Azide free (AB251166)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise