AB2859

Anti-KAT13A / SRC1 antibody

Name: Anti-KAT13A / SRC1 antibody (ab2859) | Abcam
Brand: Abcam Limited
SKU: AB2859
Price: 565 USD
Availability: InStock
Rating: 4 (5 reviews)

(5 Reviews)

(11 Publications)

Rabbit Polyclonal KAT13A / SRC1 antibody. Suitable for ICC, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human NCOA1 aa 1-50.

View Alternative Names

BHLHE74, SRC1, NCOA1, Nuclear receptor coactivator 1, NCoA-1, Class E basic helix-loop-helix protein 74, Protein Hin-2, RIP160, Renal carcinoma antigen NY-REN-52, Steroid receptor coactivator 1, bHLHe74, SRC-1

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-KAT13A / SRC1 antibody (AB2859)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KAT13A / SRC1 antibody (AB2859)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.25% Triton™ X-100 permeabilized, 70% confluent log phase A549 (Human lung carcinoma cell line) cells, blocked with 3% BSA-PBS for 1 hour at room temperature. Labelling KAT13A / SRC1 with ab2859 at 1 μg/mL and incubated for 3 hours at room temperature then labelled with secondary antibody goat anti-rabbit igG, Alexa Fluor® 488 conjugate at a 1/2000 dilution for 45 minutes at room temperature (Green). Nuclei stained with DAPI (Blue). F-actin stained with Alexa Fluor® 555 Rhodamine Phalloidin at a dilution of 1/300 (Red). Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Supplier Data

Immunocytochemistry - Anti-KAT13A / SRC1 antibody (AB2859)

Immunolocalization of SRC1 in HeLa cells using ab2859.

Immunohistochemistry (Frozen sections) - Anti-KAT13A / SRC1 antibody (AB2859)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-KAT13A / SRC1 antibody (AB2859)

Immunohistochemitry (Frozen sections) analysis of SRC1 in the Dentate Gyrus region of the rat brain using ab2859.

IHC-Fr

AbReview7518****

Immunohistochemistry (Frozen sections) - Anti-KAT13A / SRC1 antibody (AB2859)

ab2859 at 1/2000 staining mouse brain tissue sections by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 72 hours. A biotinylated goat anti-rabbit antibody was used as the secondary.

This image is courtesy of an Abreview submitted by Dr Jiqiang Zhang

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC, ICC/IF, IHC-Fr

applications

Immunogen

Synthetic Peptide within Human NCOA1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q15788

Specificity

Detects steroid receptor coactivator-1 (SRC-1).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1 µg/mL", "IHCFr-species-notes": "" }, "Rat": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1 µg/mL", "IHCFr-species-notes": "" }, "Pig": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KAT13A also known as SRC1 or nuclear receptor coactivator 1 (NCOA1) is a coactivator protein with an approximate mass of 160 kDa. This protein aids in the function of nuclear receptors and exerts its mechanical roles by interacting with transcription factors facilitating transcriptional activation. KAT13A is widely expressed in tissues such as the liver skeletal muscle and brain highlighting its ubiquitous role in cellular processes.

Biological function summary

KAT13A modulates gene expression by forming part of multi-protein complexes significantly affecting cellular functions like growth and differentiation. The protein serves as a bridge between nuclear receptors and the transcriptional machinery effectively enhancing transcription upon binding. It also plays a role in steroid hormone responses impacting pathways involved in metabolism and inflammation.

Pathways

KAT13A participates in the steroid hormone receptor signaling pathway and the PPAR signaling pathway. Within these pathways it interacts with proteins such as the estrogen receptor and PPAR-alpha contributing to energy homeostasis and regulating lipid metabolism. These interactions position KAT13A as an important player in integrating hormonal signals with transcriptional response.

KAT13A's dysregulation associates with cancer particularly breast cancer and metabolic disorders like obesity. Its relationship with the estrogen receptor links KAT13A to hormone-dependent cancers affecting cancer cell proliferation. Additionally changes in PPAR-alpha interaction relate to metabolic syndrome influencing lipid metabolism and contributing to the development of obesity-related complications.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3.

See full target information NCOA1

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in endocrinology 15:1380047 PubMed39184142

2024

Cadmium activation of wild-type and constitutively active estrogen receptor alpha.

Applications

Unspecified application

Species

Unspecified reactive species

John B Psaltis,Qiaochu Wang,Gai Yan,Reem Gahtani,Nanxi Huang,Bassem R Haddad,Mary Beth Martin

Frontiers in pharmacology 12:784822 PubMed35140604

2022

Confirmation of inhibitingTLR4/MyD88/NF-κB Signalling Pathway by Duhuo Jisheng Decoction on Osteoarthritis: A Network Pharmacology Approach-Integrated Experimental Study.

Applications

Unspecified application

Species

Unspecified reactive species

Linglong Liu,Limei Xu,Shengjie Wang,Lili Wang,Xiaoning Wang,Huifeng Xu,Xihai Li,Hongzhi Ye

Advanced science (Weinheim, Baden-Wurttemberg, Ger 7:2002273 PubMed33173745

2020

The Nuclear Orphan Receptor NR2F6 Promotes Hepatic Steatosis through Upregulation of Fatty Acid Transporter CD36.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Zhou,Lijing Jia,Zhijian Zhang,Liping Xiang,Youwen Yuan,Peilin Zheng,Bin Liu,Xingxing Ren,Hua Bian,Liwei Xie,Yao Li,Jieli Lu,Huijie Zhang,Yan Lu

Cellular and molecular life sciences : CMLS 78:317-335 PubMed32232515

2020

Mutation of a single amino acid of pregnane X receptor switches an antagonist to agonist by altering AF-2 helix positioning.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew D Huber,William C Wright,Wenwei Lin,Kinjal Majumder,Jonathan A Low,Jing Wu,Cameron D Buchman,David J Pintel,Taosheng Chen

Molecular metabolism 29:1-11 PubMed31668381

2019

Roux-en-Y gastric bypass enhances insulin secretion in type 2 diabetes via FXR-mediated TRPA1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangchen Kong,Yifan Tu,Bingfeng Li,Longmei Zhang,Linxian Feng,Lixiang Wang,Lin Zhang,Huarong Zhou,Xianxin Hua,Xiaosong Ma

Journal of diabetes research 2019:8915818 PubMed31485455

2019

FXR Mediates Adenylyl Cyclase 8 Expression in Pancreatic -Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangchen Kong,Bingfeng Li,Yushen Deng,Xiaosong Ma

Cancers 11: PubMed30736340

2019

Tumor-Associated Macrophages Induce Endocrine Therapy Resistance in ER+ Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Andrés M Castellaro,María C Rodriguez-Baili,Cecilia E Di Tada,Germán A Gil

BMC cancer 16:882 PubMed27842582

2016

Leptin and insulin up-regulate miR-4443 to suppress NCOA1 and TRAF4, and decrease the invasiveness of human colon cancer cells.

Applications

Species

Human

Ari Meerson,Hila Yehuda

Nature 456:663-6 PubMed19005469

2008

Regulation of ERBB2 by oestrogen receptor-PAX2 determines response to tamoxifen.

Applications

WB, ChIP

Species

Human, Human

Antoni Hurtado,Kelly A Holmes,Timothy R Geistlinger,Iain R Hutcheson,Robert I Nicholson,Myles Brown,Jie Jiang,William J Howat,Simak Ali,Jason S Carroll

Cell death and differentiation 15:471-83 PubMed18064044

2007

The upregulation of GLAST-1 is an indirect antiapoptotic mechanism of GDNF and neurturin in the adult CNS.

Applications

Unspecified application

Species

Unspecified reactive species

P D Koeberle,M Bähr

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KAT13A / SRC1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-KAT13A / SRC1 antibody (AB2859)

Immunocytochemistry - Anti-KAT13A / SRC1 antibody (AB2859)

Immunohistochemistry (Frozen sections) - Anti-KAT13A / SRC1 antibody (AB2859)

Immunohistochemistry (Frozen sections) - Anti-KAT13A / SRC1 antibody (AB2859)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise