AB5407

Anti-KAT13A / SRC1 antibody

Name: Anti-KAT13A / SRC1 antibody (ab5407) | Abcam
Brand: Abcam Limited
SKU: AB5407
Price: 525 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(12 Publications)

Rabbit Polyclonal KAT13A / SRC1 antibody. Suitable for WB, IHC-P, IP and reacts with Mouse, Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human NCOA1 aa 600-650.

View Alternative Names

BHLHE74, SRC1, NCOA1, Nuclear receptor coactivator 1, NCoA-1, Class E basic helix-loop-helix protein 74, Protein Hin-2, RIP160, Renal carcinoma antigen NY-REN-52, Steroid receptor coactivator 1, bHLHe74, SRC-1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT13A / SRC1 antibody (AB5407)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT13A / SRC1 antibody (AB5407)

Immunohistochemical analysis of paraffin-embedded Human prostate carcinoma labeling KAT13A/SRC1 with ab5407 at 1/1000 dilution.

Detection : DAB.

Counter stained with Hematoxylin.

Supplier Data

Western blot - Anti-KAT13A / SRC1 antibody (AB5407)

Detection : Chemiluminescence

Exposure time : 30 seconds

All lanes:

Western blot - Anti-KAT13A / SRC1 antibody (ab5407) at 0.1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 5 µg

Lane 4:

HeLa cells at 50 µg

Lane 5:

Mouse NIH3T3 cells at 50 µg

Predicted band size: 156 kDa

false

Supplier Data

Immunoprecipitation - Anti-KAT13A / SRC1 antibody (AB5407)

KAT13A/SRC1 was immunoprecipitated from 1mg of 293T cells prepared using NETN lysis buffer.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-KAT13A / SRC1 antibody (ab5407)

Predicted band size: 156 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, IP, WB

applications

Immunogen

Synthetic Peptide within Human NCOA1 aa 600-650. The exact immunogen used to generate this antibody is proprietary information.

Q15788

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KAT13A also known as SRC1 or nuclear receptor coactivator 1 (NCOA1) is a coactivator protein with an approximate mass of 160 kDa. This protein aids in the function of nuclear receptors and exerts its mechanical roles by interacting with transcription factors facilitating transcriptional activation. KAT13A is widely expressed in tissues such as the liver skeletal muscle and brain highlighting its ubiquitous role in cellular processes.

Biological function summary

KAT13A modulates gene expression by forming part of multi-protein complexes significantly affecting cellular functions like growth and differentiation. The protein serves as a bridge between nuclear receptors and the transcriptional machinery effectively enhancing transcription upon binding. It also plays a role in steroid hormone responses impacting pathways involved in metabolism and inflammation.

Pathways

KAT13A participates in the steroid hormone receptor signaling pathway and the PPAR signaling pathway. Within these pathways it interacts with proteins such as the estrogen receptor and PPAR-alpha contributing to energy homeostasis and regulating lipid metabolism. These interactions position KAT13A as an important player in integrating hormonal signals with transcriptional response.

KAT13A's dysregulation associates with cancer particularly breast cancer and metabolic disorders like obesity. Its relationship with the estrogen receptor links KAT13A to hormone-dependent cancers affecting cancer cell proliferation. Additionally changes in PPAR-alpha interaction relate to metabolic syndrome influencing lipid metabolism and contributing to the development of obesity-related complications.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3.

See full target information NCOA1

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Acta biochimica et biophysica Sinica 55:633-648 PubMed36916297

2023

Ginsenoside Rh2 suppresses colon cancer growth by targeting the miR-150-3p/SRCIN1/Wnt axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shipeng Li,Wenfeng Han,Qichen He,Yang Wang,Gang Jin,Youcheng Zhang

Disease markers 2022:4842454 PubMed36033827

2022

miR-657 Targets SRCIN1 via the Slug Pathway to Promote NSCLC Tumor Growth and EMT Induction.

Applications

Unspecified application

Species

Unspecified reactive species

Yingqian Zhang,Jiao Yuan,Mengfei Guo,Run Xiang,Xiang Wang,Tianpeng Xie,Xiang Zhuang,Qiang Li,Qi Lai

Zoological research 43:442-456 PubMed35503560

2022

Characterization of two novel knock-in mouse models of syndromic retinal ciliopathy carrying hypomorphic mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Lin Ren,Hou-Bin Zhang,Lin Li,Zheng-Lin Yang,Li Jiang

eNeuro 8: PubMed34475267

2021

Photoreceptor cKO of OTX2 Enhances OTX2 Intercellular Transfer in the Retina and Causes Photophobia.

Applications

Unspecified application

Species

Unspecified reactive species

Pasquale Pensieri,Annabelle Mantilleri,Damien Plassard,Takahisa Furukawa,Kenneth L Moya,Alain Prochiantz,Thomas Lamonerie

Investigative ophthalmology & visual science 61:14 PubMed32658961

2020

A New Cerkl Mouse Model Generated by CRISPR-Cas9 Shows Progressive Retinal Degeneration and Altered Morphological and Electrophysiological Phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Elena B Domènech,Rosa Andrés,M José López-Iniesta,Serena Mirra,Rocío García-Arroyo,Santiago Milla,Florentina Sava,Jordi Andilla,Pablo Loza-Álvarez,Pedro de la Villa,Roser Gonzàlez-Duarte,Gemma Marfany

The Journal of experimental biology 223: PubMed32371443

2020

Spectral sensitivity of cone vision in the diurnal murid .

Applications

Unspecified application

Species

Unspecified reactive species

Annette E Allen,Joshua W Mouland,Jessica Rodgers,Beatriz Baño-Otálora,Ronald H Douglas,Glen Jeffery,Anthony A Vugler,Timothy M Brown,Robert J Lucas

Translational cancer research 9:603-612 PubMed35117405

2020

SRC-1 and Twist1 are prognostic indicators of liver cancer and are associated with cell viability, invasion, migration and epithelial-mesenchymal transformation of hepatocellular carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Peng-Wei Zhao,Jia-Wei Zhang,Yan Liu,Yan Liu,Jiang-Wei Liu,Jian-Zhao Huang

eLife 8: PubMed31451149

2019

Merging organoid and organ-on-a-chip technology to generate complex multi-layer tissue models in a human retina-on-a-chip platform.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin Achberger,Christopher Probst,Jasmin Haderspeck,Sylvia Bolz,Julia Rogal,Johanna Chuchuy,Marina Nikolova,Virginia Cora,Lena Antkowiak,Wadood Haq,Nian Shen,Katja Schenke-Layland,Marius Ueffing,Stefan Liebau,Peter Loskill

Journal of cellular physiology 234:18801-18812 PubMed30924157

2019

Disease mechanisms and neuroprotection by tauroursodeoxycholic acid in Rpgr knockout mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xun Zhang,Uma Shahani,James Reilly,Xinhua Shu

Molecular medicine reports 18:2171-2181 PubMed29901151

2018

Eph/ephrin signalling serves a bidirectional role in lipopolysaccharide‑induced intestinal injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Xiong,Kai-Xue Li,Hong Wei,Lu Jiao,Shao-Yong Yu,Li Zeng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KAT13A / SRC1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT13A / SRC1 antibody (AB5407)

Western blot - Anti-KAT13A / SRC1 antibody (AB5407)

Immunoprecipitation - Anti-KAT13A / SRC1 antibody (AB5407)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

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