JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB313616

Anti-KAT13A / SRC1 antibody [EPR25219-158]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal KAT13A / SRC1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

BHLHE74, SRC1, NCOA1, Nuclear receptor coactivator 1, NCoA-1, Class E basic helix-loop-helix protein 74, Protein Hin-2, RIP160, Renal carcinoma antigen NY-REN-52, Steroid receptor coactivator 1, bHLHe74, SRC-1

2 Images
Western blot - Anti-KAT13A / SRC1 antibody [EPR25219-158] (AB313616)
  • WB

Supplier Data

Western blot - Anti-KAT13A / SRC1 antibody [EPR25219-158] (AB313616)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The bands beneath the target band (180 kDa) are likely to be degraded target fragments. Lysates were freshly made and used immediately to minimize protein degradation.

All lanes:

Western blot - Anti-KAT13A / SRC1 antibody [EPR25219-158] (ab313616) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse testis tissue lysate at 20 µg

Lane 3:

Rat brain tissue lysate at 20 µg

Lane 4:

Rat testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 180 kDa

false

Exposure time: 3min

Western blot - Anti-KAT13A / SRC1 antibody [EPR25219-158] (AB313616)
  • WB

Supplier Data

Western blot - Anti-KAT13A / SRC1 antibody [EPR25219-158] (AB313616)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The identity of the lower MW band at approximately 40 kDa is unknown. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-KAT13A / SRC1 antibody [EPR25219-158] (ab313616) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with scrambled siRNA control, whole cell lysate at 20 µg

Lane 2:

293T transfected with siRNAs specifically targeting NCOA1 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 180 kDa

false

Exposure time: 3min

  • Carrier free

    Anti-KAT13A / SRC1 antibody [EPR25219-158] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25219-158

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB176323

Anti-LXR alpha antibody [EPR6508(N)]

RabMAb|Recombinant

Western blot - Anti-LXR alpha antibody [EPR6508(N)] (AB176323)

  • 4
  • 3
Primary Antibodies

AB79756

Anti-c-Jun (phospho T91) antibody [EPR2236]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-c-Jun (phospho T91) antibody [EPR2236] (AB79756)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KAT13A also known as SRC1 or nuclear receptor coactivator 1 (NCOA1) is a coactivator protein with an approximate mass of 160 kDa. This protein aids in the function of nuclear receptors and exerts its mechanical roles by interacting with transcription factors facilitating transcriptional activation. KAT13A is widely expressed in tissues such as the liver skeletal muscle and brain highlighting its ubiquitous role in cellular processes.
Biological function summary

KAT13A modulates gene expression by forming part of multi-protein complexes significantly affecting cellular functions like growth and differentiation. The protein serves as a bridge between nuclear receptors and the transcriptional machinery effectively enhancing transcription upon binding. It also plays a role in steroid hormone responses impacting pathways involved in metabolism and inflammation.

Pathways

KAT13A participates in the steroid hormone receptor signaling pathway and the PPAR signaling pathway. Within these pathways it interacts with proteins such as the estrogen receptor and PPAR-alpha contributing to energy homeostasis and regulating lipid metabolism. These interactions position KAT13A as an important player in integrating hormonal signals with transcriptional response.

KAT13A's dysregulation associates with cancer particularly breast cancer and metabolic disorders like obesity. Its relationship with the estrogen receptor links KAT13A to hormone-dependent cancers affecting cancer cell proliferation. Additionally changes in PPAR-alpha interaction relate to metabolic syndrome influencing lipid metabolism and contributing to the development of obesity-related complications.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3.
See full target information NCOA1
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com