Rabbit Recombinant Monoclonal KAT13A / SRC1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-KAT13A / SRC1 antibody [EPR25219-158] - BSA and Azide free (AB313617), expandable thumbnail](https://content.abcam.com/products/images/kat13a-src1-antibody-epr25219-158-bsa-and-azide-free-ab313617--western-blot-img421008.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-KAT13A / SRC1 antibody [EPR25219-158] - BSA and Azide free (AB313617)")
![Western blot - Anti-KAT13A / SRC1 antibody [EPR25219-158] - BSA and Azide free (AB313617), expandable thumbnail](https://content.abcam.com/products/images/kat13a-src1-antibody-epr25219-158-bsa-and-azide-free-ab313617--western-blot-img421007.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-KAT13A / SRC1 antibody [EPR25219-158] - BSA and Azide free (AB313617)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IP | |
|---|---|---|
| Human | Tested | Not recommended |
| Mouse | Tested | Not recommended |
| Rat | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3.
Alternative names
BHLHE74, SRC1, NCOA1, Nuclear receptor coactivator 1, NCoA-1, Class E basic helix-loop-helix protein 74, Protein Hin-2, RIP160, Renal carcinoma antigen NY-REN-52, Steroid receptor coactivator 1, bHLHe74, SRC-1
Recommended products
Rabbit Recombinant Monoclonal KAT13A / SRC1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR25219-158
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab313617 is the carrier-free version of Anti-KAT13A / SRC1 antibody [EPR25219-158] ab313616.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
KAT13A also known as SRC1 or nuclear receptor coactivator 1 (NCOA1) is a coactivator protein with an approximate mass of 160 kDa. This protein aids in the function of nuclear receptors and exerts its mechanical roles by interacting with transcription factors facilitating transcriptional activation. KAT13A is widely expressed in tissues such as the liver skeletal muscle and brain highlighting its ubiquitous role in cellular processes.
- Biological function summary
KAT13A modulates gene expression by forming part of multi-protein complexes significantly affecting cellular functions like growth and differentiation. The protein serves as a bridge between nuclear receptors and the transcriptional machinery effectively enhancing transcription upon binding. It also plays a role in steroid hormone responses impacting pathways involved in metabolism and inflammation.
- Pathways
KAT13A participates in the steroid hormone receptor signaling pathway and the PPAR signaling pathway. Within these pathways it interacts with proteins such as the estrogen receptor and PPAR-alpha contributing to energy homeostasis and regulating lipid metabolism. These interactions position KAT13A as an important player in integrating hormonal signals with transcriptional response.
- Associated diseases and disorders
KAT13A's dysregulation associates with cancer particularly breast cancer and metabolic disorders like obesity. Its relationship with the estrogen receptor links KAT13A to hormone-dependent cancers affecting cancer cell proliferation. Additionally changes in PPAR-alpha interaction relate to metabolic syndrome influencing lipid metabolism and contributing to the development of obesity-related complications.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-KAT13A / SRC1 antibody [EPR25219-158] - BSA and Azide free (ab313617), expandable thumbnail](https://content.abcam.com/products/images/kat13a-src1-antibody-epr25219-158-bsa-and-azide-free-ab313617--western-blot-img421008.jpg "Western blot - Anti-KAT13A / SRC1 antibody [EPR25219-158] - BSA and Azide free (ab313617)")
Western blot - Anti-KAT13A / SRC1 antibody [EPR25219-158] - BSA and Azide free (ab313617)
This data was developed using Anti-KAT13A / SRC1 antibody [EPR25219-158] ab313616, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The bands beneath the target band (180 kDa) are likely to be degraded target fragments.
Lysates were freshly made and used immediately to minimize protein degradation.All lanes: Western blot - Anti-KAT13A / SRC1 antibody [EPR25219-158] (Anti-KAT13A / SRC1 antibody [EPR25219-158] ab313616) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse testis tissue lysate at 20 µg
Lane 3: Rat brain tissue lysate at 20 µg
Lane 4: Rat testis tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 180 kDa
Exposure time: 3min
![Western blot - Anti-KAT13A / SRC1 antibody [EPR25219-158] - BSA and Azide free (ab313617), expandable thumbnail](https://content.abcam.com/products/images/kat13a-src1-antibody-epr25219-158-bsa-and-azide-free-ab313617--western-blot-img421007.jpg "Western blot - Anti-KAT13A / SRC1 antibody [EPR25219-158] - BSA and Azide free (ab313617)")
Western blot - Anti-KAT13A / SRC1 antibody [EPR25219-158] - BSA and Azide free (ab313617)
This data was developed using Anti-KAT13A / SRC1 antibody [EPR25219-158] ab313616, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The identity of the lower MW band at approximately 40 kDa is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.All lanes: Western blot - Anti-KAT13A / SRC1 antibody [EPR25219-158] (Anti-KAT13A / SRC1 antibody [EPR25219-158] ab313616) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) transfected with scrambled siRNA control, whole cell lysate at 20 µg
Lane 2: 293T transfected with siRNAs specifically targeting NCOA1 whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 180 kDa
Exposure time: 3min
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