Rabbit Polyclonal KAT2/AadAT antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial aa 50-200.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/2500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
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Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino-group acceptors, with a preference for 2-oxoglutarate, 2-oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro).
KAT2, KYAT2, KYAT2, KAT2, AADAT, KAT/AadAT, 2-aminoadipate aminotransferase, 2-aminoadipate transaminase, Alpha-aminoadipate aminotransferase, Glycine transaminase AADAT, Kynurenine aminotransferase II, Kynurenine--glyoxylate transaminase AADAT, Kynurenine--oxoglutarate aminotransferase II, Kynurenine--oxoglutarate transaminase 2, Kynurenine--oxoglutarate transaminase II, Methionine--glyoxylate transaminase AADAT, AadAT
Rabbit Polyclonal KAT2/AadAT antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial aa 50-200.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
KAT2 also known as AadAT is an important lysine acetyltransferase enzyme that modifies proteins by adding acetyl groups to lysine residues. This process impacts several cellular functions. KAT2 typically features a molecular mass of approximately 55 kDa. You will usually find it expressed in a wide range of tissues including liver and brain. The enzyme's presence suggests an essential role in numerous physiological processes particularly within the nucleus where it modifies histones.
KAT2/AadAT takes part in chromatin remodeling and gene expression regulation. This enzyme often functions as part of multiprotein complexes which include transcription factors and coactivators. The acetylation of histone proteins by KAT2 modifies chromatin structure resulting in enhanced access of transcription machinery to DNA. This activity is essential for processes like cell growth and differentiation playing a significant role in maintaining normal physiological conditions.
KAT2/AadAT is integrated into the larger network of acetylation-mediated gene regulation. Two primary pathways it is involved with include the gene expression pathway and the chromatin modification pathway. In these pathways KAT2 interacts with proteins such as CBP/p300 which act as transcriptional coactivators. This interaction helps facilitate the acetylation process that is vital for gene activation and the subsequent biological responses to external stimuli.
Alterations in KAT2/AadAT activity link to specific conditions including cancer and neurodegenerative diseases. For instance abnormal KAT2 activity has been observed in certain cancers where dysregulated gene expression promotes tumor progression. Additionally altered acetylation patterns by KAT2 can contribute to neurodegenerative disorders where the modified interaction with proteins like HDAC1 disrupts normal neuronal function.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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PFA fixed, Triton X-100 permeabilized A-431 (Human epidermoid carcinoma cell line) cells labeling KAT2/AadAT using ab254661 at 4µg/ml (green) in ICC/IF.
Paraffin-embedded human liver tissue stained for KAT2/AadAT using ab254661 at 1/1000 dilution in immunohistochemical analysis.
Lane 1: Western blot - Anti-KAT2/AadAT antibody (ab254661)
Lane 2: Western blot - Anti-KAT2/AadAT antibody (ab254661) at 0.4 µg/mL
All lanes: RT4 (Human urinary bladder cancer cell line) whole cell lysate
Predicted band size: 47 kDa
Paraffin-embedded human pancreas tissue stained for KAT2/AadAT using ab254661 at 1/1000 dilution in immunohistochemical analysis. Low expression, as expected.
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