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AB254661

Anti-KAT2/AadAT antibody

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(2 Publications)

Rabbit Polyclonal KAT2/AadAT antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AADAT aa 50-200.

View Alternative Names

KAT2, KYAT2, AADAT, KAT/AadAT, 2-aminoadipate aminotransferase, 2-aminoadipate transaminase, Alpha-aminoadipate aminotransferase, Glycine transaminase AADAT, Kynurenine aminotransferase II, Kynurenine--glyoxylate transaminase AADAT, Kynurenine--oxoglutarate aminotransferase II, Kynurenine--oxoglutarate transaminase 2, Kynurenine--oxoglutarate transaminase II, Methionine--glyoxylate transaminase AADAT, AadAT

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT2/AadAT antibody (AB254661)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT2/AadAT antibody (AB254661)

Paraffin-embedded human pancreas tissue stained for KAT2/AadAT using ab254661 at 1/1000 dilution in immunohistochemical analysis. Low expression, as expected.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT2/AadAT antibody (AB254661)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT2/AadAT antibody (AB254661)

Paraffin-embedded human liver tissue stained for KAT2/AadAT using ab254661 at 1/1000 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-KAT2/AadAT antibody (AB254661)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-KAT2/AadAT antibody (AB254661)

PFA fixed, Triton X-100 permeabilized A-431 (Human epidermoid carcinoma cell line) cells labeling KAT2/AadAT using ab254661 at 4µg/ml (green) in ICC/IF.

Western blot - Anti-KAT2/AadAT antibody (AB254661)
  • WB

Supplier Data

Western blot - Anti-KAT2/AadAT antibody (AB254661)

Lane 1:

Western blot - Anti-KAT2/AadAT antibody (ab254661)

Lane 2:

Western blot - Anti-KAT2/AadAT antibody (ab254661) at 0.4 µg/mL

All lanes:

RT4 (Human urinary bladder cancer cell line) whole cell lysate

Predicted band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human AADAT aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q8N5Z0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KAT2 also known as AadAT is an important lysine acetyltransferase enzyme that modifies proteins by adding acetyl groups to lysine residues. This process impacts several cellular functions. KAT2 typically features a molecular mass of approximately 55 kDa. You will usually find it expressed in a wide range of tissues including liver and brain. The enzyme's presence suggests an essential role in numerous physiological processes particularly within the nucleus where it modifies histones.
Biological function summary

KAT2/AadAT takes part in chromatin remodeling and gene expression regulation. This enzyme often functions as part of multiprotein complexes which include transcription factors and coactivators. The acetylation of histone proteins by KAT2 modifies chromatin structure resulting in enhanced access of transcription machinery to DNA. This activity is essential for processes like cell growth and differentiation playing a significant role in maintaining normal physiological conditions.

Pathways

KAT2/AadAT is integrated into the larger network of acetylation-mediated gene regulation. Two primary pathways it is involved with include the gene expression pathway and the chromatin modification pathway. In these pathways KAT2 interacts with proteins such as CBP/p300 which act as transcriptional coactivators. This interaction helps facilitate the acetylation process that is vital for gene activation and the subsequent biological responses to external stimuli.

Alterations in KAT2/AadAT activity link to specific conditions including cancer and neurodegenerative diseases. For instance abnormal KAT2 activity has been observed in certain cancers where dysregulated gene expression promotes tumor progression. Additionally altered acetylation patterns by KAT2 can contribute to neurodegenerative disorders where the modified interaction with proteins like HDAC1 disrupts normal neuronal function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino-group acceptors, with a preference for 2-oxoglutarate, 2-oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro).
See full target information AADAT
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Histology and histopathology 40:1295-1307 PubMed39698827

2024

TRIM22 mechanism promoting KAT2A ubiquitination degradation to regulate ferroptosis in hepatocellular carcinoma cell invasion and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wang,Xiaoshan Chen,Wei Wei

Molecular & cellular proteomics : MCP 23:100838 PubMed39251023

2024

Proteome Dynamics in iPSC-Derived Human Dopaminergic Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Cavarischia-Rega,Karan Sharma,Julia C Fitzgerald,Boris Macek
View all publications
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