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Rabbit Polyclonal KAT2/AadAT antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial aa 50-200.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-KAT2/AadAT antibody (AB254661), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT2/AadAT antibody (AB254661), expandable thumbnail
  • Western blot - Anti-KAT2/AadAT antibody (AB254661), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT2/AadAT antibody (AB254661), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial aa 50-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q8N5Z0

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/1000.00000 - 1/2500.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

0.04000-0.40000 µg/mL

Notes

-

Tested
Tested

Species

Human

Dilution info

0.25000-2.00000 µg/mL

Notes

Fixation/Permeabilization: PFA/Triton X-100.

Associated Products

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Target data

Function

Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino-group acceptors, with a preference for 2-oxoglutarate, 2-oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro).

Alternative names

Recommended products

Rabbit Polyclonal KAT2/AadAT antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial aa 50-200.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial aa 50-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q8N5Z0
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

KAT2 also known as AadAT is an important lysine acetyltransferase enzyme that modifies proteins by adding acetyl groups to lysine residues. This process impacts several cellular functions. KAT2 typically features a molecular mass of approximately 55 kDa. You will usually find it expressed in a wide range of tissues including liver and brain. The enzyme's presence suggests an essential role in numerous physiological processes particularly within the nucleus where it modifies histones.

Biological function summary

KAT2/AadAT takes part in chromatin remodeling and gene expression regulation. This enzyme often functions as part of multiprotein complexes which include transcription factors and coactivators. The acetylation of histone proteins by KAT2 modifies chromatin structure resulting in enhanced access of transcription machinery to DNA. This activity is essential for processes like cell growth and differentiation playing a significant role in maintaining normal physiological conditions.

Pathways

KAT2/AadAT is integrated into the larger network of acetylation-mediated gene regulation. Two primary pathways it is involved with include the gene expression pathway and the chromatin modification pathway. In these pathways KAT2 interacts with proteins such as CBP/p300 which act as transcriptional coactivators. This interaction helps facilitate the acetylation process that is vital for gene activation and the subsequent biological responses to external stimuli.

Associated diseases and disorders

Alterations in KAT2/AadAT activity link to specific conditions including cancer and neurodegenerative diseases. For instance abnormal KAT2 activity has been observed in certain cancers where dysregulated gene expression promotes tumor progression. Additionally altered acetylation patterns by KAT2 can contribute to neurodegenerative disorders where the modified interaction with proteins like HDAC1 disrupts normal neuronal function.

Product promise

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4 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-KAT2/AadAT antibody (ab254661), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-KAT2/AadAT antibody (ab254661)

    PFA fixed, Triton X-100 permeabilized A-431 (Human epidermoid carcinoma cell line) cells labeling KAT2/AadAT using ab254661 at 4µg/ml (green) in ICC/IF.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT2/AadAT antibody (ab254661), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT2/AadAT antibody (ab254661)

    Paraffin-embedded human liver tissue stained for KAT2/AadAT using ab254661 at 1/1000 dilution in immunohistochemical analysis.

  • Western blot - Anti-KAT2/AadAT antibody (ab254661), expandable thumbnail

    Western blot - Anti-KAT2/AadAT antibody (ab254661)

    Lane 1: Western blot - Anti-KAT2/AadAT antibody (ab254661)

    Lane 2: Western blot - Anti-KAT2/AadAT antibody (ab254661) at 0.4 µg/mL

    All lanes: RT4 (Human urinary bladder cancer cell line) whole cell lysate

    Predicted band size: 47 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT2/AadAT antibody (ab254661), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT2/AadAT antibody (ab254661)

    Paraffin-embedded human pancreas tissue stained for KAT2/AadAT using ab254661 at 1/1000 dilution in immunohistochemical analysis. Low expression, as expected.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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