AB326697

Anti-KAT2B antibody [SR3831]

Recombinant
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal KAT2B / PCAF antibody. Suitable for WB and reacts with Human, Mouse samples.

View Alternative Names

PCAF, KAT2B, Histone acetyltransferase KAT2B, Histone acetyltransferase PCAF, Lysine acetyltransferase 2B, P300/CBP-associated factor, Spermidine acetyltransferase KAT2B, Histone acetylase PCAF, P/CAF

2 Images

Supplier Data

Western blot - Anti-KAT2B antibody [SR3831] (AB326697)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : A431 whole cell lysates
Lane 2 : 3T3 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size : 93 kDa
Observed band size : 93 kDa

Exposure time : 4 seconds

All lanes:

Western blot - Anti-KAT2B antibody [SR3831] (ab326697) at 1/1000 dilution

Lane 1:

A431 whole cell lysates at 20 µg

Lane 2:

3T3 whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 93 kDa

Observed band size: 93 kDa

false

Exposure time: 4s

Supplier Data

Western blot - Anti-KAT2B antibody [SR3831] (AB326697)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : Wild-type HT-1080 cell lysate
Lane 2 : PCAF Rabbit mAb knockdown HT-1080 cell lysate

Lysates/proteins at 20 µg per lane.

All lanes:

Western blot - Anti-KAT2B antibody [SR3831] (ab326697) at 1/1000 dilution

Lane 1:

Wild-type HT-1080 cell lysate at 20 µg

Lane 2:

PCAF Rabbit mAb knockdown HT-1080 cell lysate at 20 µg

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SR3831

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }

style

left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Functions as a histone acetyltransferase (HAT) to promote transcriptional activation (PubMed : 8945521). Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles (PubMed : 8945521). Has a a strong preference for acetylation of H3 at 'Lys-9' (H3K9ac) (PubMed : 21131905). Also acetylates non-histone proteins, such as ACLY, MAPRE1/EB1, PLK4, RRP9/U3-55K and TBX5 (PubMed : 10675335, PubMed : 23001180, PubMed : 23932781, PubMed : 26867678, PubMed : 27796307, PubMed : 29174768, PubMed : 9707565). Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A (PubMed : 8684459). Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators : NPAS2-BMAL1 and CLOCK-BMAL1 heterodimers (PubMed : 14645221). Involved in heart and limb development by mediating acetylation of TBX5, acetylation regulating nucleocytoplasmic shuttling of TBX5 (PubMed : 29174768). Acts as a negative regulator of centrosome amplification by mediating acetylation of PLK4 (PubMed : 27796307). Acetylates RRP9/U3-55K, a core subunit of the U3 snoRNP complex, impairing pre-rRNA processing (PubMed : 26867678). Acetylates MAPRE1/EB1, promoting dynamic kinetochore-microtubule interactions in early mitosis (PubMed : 23001180). Also acetylates spermidine (PubMed : 27389534).. (Microbial infection) In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes.

See full target information KAT2B

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com