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AB168346

Anti-KAT4 / TBP Associated Factor 1 antibody [EPR7145(2)]

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(3 Publications)

Rabbit Recombinant Monoclonal TAF1 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

BA2R, CCG1, CCGS, TAF2A, TAF1, Transcription initiation factor TFIID subunit 1, Cell cycle gene 1 protein, TBP-associated factor 250 kDa, Transcription initiation factor TFIID 250 kDa subunit, p250, TAF(II)250, TAFII-250, TAFII250

1 Images
Western blot - Anti-KAT4 / TBP Associated Factor 1 antibody [EPR7145(2)] (AB168346)
  • WB

Unknown

Western blot - Anti-KAT4 / TBP Associated Factor 1 antibody [EPR7145(2)] (AB168346)

All lanes:

Western blot - Anti-KAT4 / TBP Associated Factor 1 antibody [EPR7145(2)] (ab168346) at 1/1000 dilution

Lane 1:

293T lysate at 10 µg

Lane 2:

SH-SY5Y lysate at 10 µg

Lane 3:

HepG2 lysate at 10 µg

Lane 4:

Human fetal brain lysate at 10 µg

Lane 5:

Jurkat lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 213 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7145(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KAT4 also known as TBP Associated Factor 1 (TAF1) is a protein with a mass of around 240 kDa. It functions mechanically as part of the transcription machinery where it acts as an important component of the transcription factor IID (TFIID) complex. This protein is expressed in various tissues with a significant expression in the liver. TAF1 is involved in the regulation of gene expression acting as a bridge between specific DNA sequences and the general transcription factors that promote RNA polymerase II activity.
Biological function summary

TAF1 is an integral part of the TFIID complex which consists of multiple subunits. It plays a critical role in the initiation of gene transcription by facilitating the binding of the TFIID complex to RNA polymerase II and other associated factors. TAF1 contributes to the accurate transcription start site selection and the regulation of promoter activity influencing gene expression across diverse cell types.

Pathways

The role of TAF1 fits into the transcription regulation and RNA polymerase II-mediated pathways. It collaborates with proteins like TBP (TATA-binding protein) to initiate transcription. TAF1's kinase activity also involves phosphorylation processes that are part of transcription regulation mechanisms connecting it to broader signaling pathways within the cell.

TAF1 has associations with neurodevelopmental and degenerative disorders such as Parkinson's disease. Mutations or dysregulation in TAF1 can lead to abnormal transcriptional activity which impacts neuronal development and survival. The protein's interaction with other transcription-related proteins including TBP provides further insight into how transcriptional misregulation can contribute to these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed : 33795473). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed : 33795473). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed : 33795473). TAF1 is the largest component and core scaffold of the TFIID complex, involved in nucleating complex assembly (PubMed : 25412659, PubMed : 27007846, PubMed : 33795473). TAF1 forms a promoter DNA binding subcomplex of TFIID, together with TAF7 and TAF2 (PubMed : 33795473). Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors (PubMed : 25412659, PubMed : 8625415). Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53 (PubMed : 25412659). Phosphorylates GTF2A1 and GTF2F1 on Ser residues (PubMed : 25412659). Possesses DNA-binding activity (PubMed : 25412659). Essential for progression of the G1 phase of the cell cycle (PubMed : 11278496, PubMed : 15053879, PubMed : 2038334, PubMed : 8450888, PubMed : 8625415, PubMed : 9660973, PubMed : 9858607). Exhibits histone acetyltransferase activity towards histones H3 and H4 (PubMed : 15870300).
See full target information TAF1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Clinical proteomics 22:12 PubMed40229672

2025

Proteomic profiles screening identified novel exosomal protein biomarkers for diagnosis of lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Weiyan Feng,Ying Lin,Ling Zhang,Weiming Hu

International journal of environmental research an 17: PubMed32204452

2020

Impacts of Aerobic Exercise on Depression-Like Behaviors in Chronic Unpredictable Mild Stress Mice and Related Factors in the AMPK/PGC-1α Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Luo,Changfa Tang,Xiaobin Chen,Zhanbing Ren,Honglin Qu,Rong Chen,Zhen Tong

Molecular endocrinology (Baltimore, Md.) 24:696-708 PubMed20181722

2010

TAF1 differentially enhances androgen receptor transcriptional activity via its N-terminal kinase and ubiquitin-activating and -conjugating domains.

Applications

Unspecified application

Species

Unspecified reactive species

Peyman Tavassoli,Latif A Wafa,Helen Cheng,Amina Zoubeidi,Ladan Fazli,Martin Gleave,Robert Snoek,Paul S Rennie
View all publications
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Product promise

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