AB124993

Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)]

RabMAb
Recombinant
Advanced Validation
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(4 Publications)

Rabbit Recombinant Monoclonal KAT7 / Hbo1 / MYST2 antibody. Suitable for ChIC/CUT&RUN-seq, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

HBO1, HBOa, MYST2, KAT7, Histone acetyltransferase KAT7, Histone acetyltransferase binding to ORC1, Lysine acetyltransferase 7, MYST-2

6 Images

Flow Cytometry (Intracellular) - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (AB124993)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (AB124993)

ab124993 at 1/100 dilution staining KAT7 / Hbo1 / MYST2 in permeabilized HeLa cells by intracellular flow cytometry (red) or negative control (green).

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Western blot - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (AB124993)

All lanes:

Western blot - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (ab124993) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

MCF7 cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 71 kDa

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ChIC/CUT&RUN sequencing - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (AB124993)

ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (AB124993)

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (human cervix adenocarcinoma epithelial cell) cells and 5 µg of ab124993 [EPR7194(B)]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.

The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (AB124993)

The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (AB124993)

The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

OI-RD Scanning - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (AB124993)

OI-RD Scanning

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OI-RD Scanning - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (AB124993)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7194(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra), ChIC/CUT&RUN-seq

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChICCUTRUNseq" : {"fullname" : "ChIC/CUT&RUN sequencing", "shortname":"ChIC/CUT&RUN-seq"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChICCUTRUNseq-species-checked": "testedAndGuaranteed", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "ChICCUTRUNseq-species-checked": "predicted", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ChICCUTRUNseq-species-checked": "predicted", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KAT7 also known as Hbo1 KAT-7 MYST2 KAT-7 and Katset functions as a histone acetyltransferase specifically modifying histone H4 at lysine 12 5 and 8. With a molecular mass typically around 63 kDa KAT7 is expressed in nucleus and localizes to chromatin. It associates with chromatin during DNA replication and is vital in histone H4 acetylation facilitating chromatin remodeling and gene expression.

Biological function summary

This protein plays a significant role in transcription regulation and DNA replication. KAT7 is a component of the HBO1 complex working together with other proteins like JADE1/2/3 BRPF1/2/3 and ING4/5 making it integral to cell cycle progression and transcriptional activation. This complex is essential for initiating DNA replication as it acetylates histones and regulates chromatin accessibility necessary for DNA synthesis.

Pathways

KAT7/Hbo1/MYST2 is actively engaged in the transcriptional regulatory network and DNA replication pathways. It influences gene expression through its interaction with the transcriptional machinery and by modifying the chromatin structure. Within these pathways it interacts with proteins like BRPF1 and ING5 enhancing transcription and replication. This positions KAT7 as an important component in ensuring cellular growth and division through its histone acetylation activities.

Research indicates a link between KAT7 function and cancer. Its dysregulation contributes to tumorigenesis related to its role in cell proliferation and gene expression. KAT7 affects the expression of oncogenes and interacts with proteins like p53. Altered KAT7 activity disrupts normal cellular processes and in cancer results in aberrant cell cycle regulation and uncontrolled cell proliferation highlighting its potential as a therapeutic target in oncology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalytic subunit of histone acetyltransferase HBO1 complexes, which specifically mediate acetylation of histone H3 at 'Lys-14' (H3K14ac), thereby regulating various processes, such as gene transcription, protein ubiquitination, immune regulation, stem cell pluripotent and self-renewal maintenance and embryonic development (PubMed : 16387653, PubMed : 21753189, PubMed : 24065767, PubMed : 26620551, PubMed : 31767635, PubMed : 31827282). Some complexes also catalyze acetylation of histone H4 at 'Lys-5', 'Lys-8' and 'Lys-12' (H4K5ac, H4K8ac and H4K12ac, respectively), regulating DNA replication initiation, regulating DNA replication initiation (PubMed : 10438470, PubMed : 19187766, PubMed : 20129055, PubMed : 24065767). Specificity of the HBO1 complexes is determined by the scaffold subunit : complexes containing BRPF scaffold (BRPF1, BRD1/BRPF2 or BRPF3) direct KAT7/HBO1 specificity towards H3K14ac, while complexes containing JADE (JADE1, JADE2 and JADE3) scaffold direct KAT7/HBO1 specificity towards histone H4 (PubMed : 19187766, PubMed : 20129055, PubMed : 24065767, PubMed : 26620551). H3K14ac promotes transcriptional elongation by facilitating the processivity of RNA polymerase II (PubMed : 31827282). Acts as a key regulator of hematopoiesis by forming a complex with BRD1/BRPF2, directing KAT7/HBO1 specificity towards H3K14ac and promoting erythroid differentiation (PubMed : 21753189). H3K14ac is also required for T-cell development (By similarity). KAT7/HBO1-mediated acetylation facilitates two consecutive steps, licensing and activation, in DNA replication initiation : H3K14ac facilitates the activation of replication origins, and histone H4 acetylation (H4K5ac, H4K8ac and H4K12ac) facilitates chromatin loading of MCM complexes, promoting DNA replication licensing (PubMed : 10438470, PubMed : 11278932, PubMed : 18832067, PubMed : 19187766, PubMed : 20129055, PubMed : 21856198, PubMed : 24065767, PubMed : 26620551). Acts as a positive regulator of centromeric CENPA assembly : recruited to centromeres and mediates histone acetylation, thereby preventing centromere inactivation mediated by SUV39H1, possibly by increasing histone turnover/exchange (PubMed : 27270040). Involved in nucleotide excision repair : phosphorylation by ATR in response to ultraviolet irradiation promotes its localization to DNA damage sites, where it mediates histone acetylation to facilitate recruitment of XPC at the damaged DNA sites (PubMed : 28719581). Acts as an inhibitor of NF-kappa-B independently of its histone acetyltransferase activity (PubMed : 16997280).. Plays a central role in the maintenance of leukemia stem cells in acute myeloid leukemia (AML) (PubMed : 31827282). Acts by mediating acetylation of histone H3 at 'Lys-14' (H3K14ac), thereby facilitating the processivity of RNA polymerase II to maintain the high expression of key genes, such as HOXA9 and HOXA10 that help to sustain the functional properties of leukemia stem cells (PubMed : 31827282).

See full target information KAT7

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e28827 PubMed38601626

2024

HBO1/KAT7/MYST2 HAT complex regulates human adenovirus replicative cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Heba Kamel,Varsha Shete,Sayikrushna Gadamsetty,Drayson Graves,Scott Bachus,Nikolas Akkerman,Peter Pelka,Bayar Thimmapaya

Cell reports 30:1478-1490.e6 PubMed32023463

2020

Oct4-Mediated Inhibition of Lsd1 Activity Promotes the Active and Primed State of Pluripotency Enhancers.

Applications

Unspecified application

Species

Unspecified reactive species

Lama AlAbdi,Debapriya Saha,Ming He,Mohd Saleem Dar,Sagar M Utturkar,Putu Ayu Sudyanti,Stephen McCune,Brice H Spears,James A Breedlove,Nadia A Lanman,Humaira Gowher

American journal of physiology. Renal physiology : PubMed30623724

2019

TGF-β1 modifies histone acetylation and acetyl-coenzyme A metabolism in renal myofibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Edward R Smith,Belinda Wigg,Stephen Holt,Timothy David Hewitson

Cell reports 7:575-587 PubMed24726359

2014

The RBBP6/ZBTB38/MCM10 axis regulates DNA replication and common fragile site stability.

Applications

Species

Unspecified reactive species

Benoit Miotto,Moredreck Chibi,Ping Xie,Stéphane Koundrioukoff,Hanlie Moolman-Smook,David Pugh,Michelle Debatisse,Fuchu He,Lingqiang Zhang,Pierre-Antoine Defossez

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)]

Flow Cytometry (Intracellular) - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (AB124993)

Western blot - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (AB124993)

ChIC/CUT&RUN sequencing - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (AB124993)

ChIC/CUT&RUN sequencing - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (AB124993)

ChIC/CUT&RUN sequencing - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (AB124993)

OI-RD Scanning - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (AB124993)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise