Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] - BSA and Azide free

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] - BSA and Azide free (AB248087)

This data was developed using ab124993, the same antibody clone in a different buffer formulation.

ab124993 at 1/100 dilution staining KAT7 / Hbo1 / MYST2 in permeabilized HeLa cells by intracellular flow cytometry (red) or negative control (green).

• WB

Unknown

Western blot - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] - BSA and Azide free (AB248087)

This data was developed using ab124993, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (<a href='/en-us/products/primary-antibodies/kat7-hbo1-myst2-antibody-epr7194b-ab124993'>ab124993</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

MCF7 cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 71 kDa

false

• ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] - BSA and Azide free (AB248087)

This data was developed using ab124993, the same antibody clone in a different buffer formulation.

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (human cervix adenocarcinoma epithelial cell) cells and 5 µg of ab124993 [EPR7194(B)]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.

The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

• ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] - BSA and Azide free (AB248087)

This data was developed using ab124993, the same antibody clone in a different buffer formulation.

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (human cervix adenocarcinoma epithelial cell) cells and 5 µg of ab124993 [EPR7194(B)]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.

The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

• ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] - BSA and Azide free (AB248087)

This data was developed using ab124993, the same antibody clone in a different buffer formulation.

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (human cervix adenocarcinoma epithelial cell) cells and 5 µg of ab124993 [EPR7194(B)]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.

The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] - BSA and Azide free (AB248087)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.