Rabbit Polyclonal KAT8 / MYST1 / MOF antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human KAT8 aa 1-200.
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Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human KAT8 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H7Z6
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
Species Mouse | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
Species Rat | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
Associated Products
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Target data
Function
Histone acetyltransferase which may be involved in transcriptional activation (PubMed:12397079, PubMed:22020126). May influence the function of ATM (PubMed:15923642). As part of the MSL complex it is involved in acetylation of nucleosomal histone H4 producing specifically H4K16ac (PubMed:16227571, PubMed:16543150, PubMed:21217699, PubMed:22547026, PubMed:22020126). As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues (PubMed:20018852, PubMed:22547026). That activity is less specific than the one of the MSL complex (PubMed:20018852, PubMed:22547026). Can also acetylate TP53/p53 at 'Lys-120'.
Alternative names
Histone acetyltransferase KAT8, Lysine acetyltransferase 8, MYST-1, hMOF, KAT8, MOF, MYST1, PP7073
Recommended products
Rabbit Polyclonal KAT8 / MYST1 / MOF antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human KAT8 aa 1-200.
Alternative names
Histone acetyltransferase KAT8, Lysine acetyltransferase 8, MYST-1, hMOF, KAT8, MOF, MYST1, PP7073
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human KAT8 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H7Z6
- Purification technique
Affinity purification Protein G
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The KAT8 protein also known as MYST1 or MOF functions as a histone acetyltransferase. It acetylates histone H4 at lysine 16 which is critical for chromatin structure and gene expression regulation. The molecular mass of KAT8 is approximately 55 kDa. This protein shows high expression in testis and is also present in other tissues. KAT8 is part of the MYST family of acetyltransferases sharing similarities with other MYST family proteins such as MOF protein.
- Biological function summary
KAT8 plays an essential role in gene transcription regulation by forming part of a complex known as the MSL (Male-Specific Lethal) complex. This complex ensures dosage compensation in Drosophila by adjusting gene expression levels. KAT8 is vital for the maintenance of chromatin structure influencing gene activation and silencing across various biological systems. It interacts with several co-factors during transcription regulation further highlighting its functional significance in the epigenetic landscape.
- Pathways
KAT8 engages in processes linked to chromatin modification and gene expression. Specifically it plays a significant role in the pathways of chromatin organization and transcriptional regulation by RNA polymerase II. The protein ENL participates in related pathways and interacts with KAT8 during transcription elongation. These interactions highlight KAT8's role in epigenetic regulation affecting multiple genes' transcription states.
- Associated diseases and disorders
KAT8 is associated with cancers and neurodevelopmental disorders. Aberrant expression or mutations in KAT8 have been observed in certain cancers making it a potential target for therapeutic intervention. In neurodevelopmental disorders changes in KAT8 acetyltransferase activity affect neuronal gene expression implicating it in disorders linked to cognitive deficits. Another protein TIP60 shows functional overlap with KAT8 in these conditions suggesting interconnected roles in disease pathogenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-KAT8 / MYST1 / MOF antibody (ab224597)
All lanes: Western blot - Anti-KAT8 / MYST1 / MOF antibody (ab224597) at 1/500 dilution
Lane 1: MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 2: HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 3: Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4: Mouse liver tissue lysate
Lane 5: Rat liver tissue lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 52 kDa
Observed band size: 53 kDa
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