AB72056

Anti-KAT8 / MYST1 / MOF antibody

BOND RX™ Validated
What is this?

Be the first to review this product! Submit a review

(5 Publications)

Rabbit Polyclonal KAT8 / MYST1 / MOF antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human KAT8 aa 1-50.

View Alternative Names

MOF, MYST1, PP7073, KAT8, Histone acetyltransferase KAT8, Lysine acetyltransferase 8, Males-absent on the first protein homolog, Protein acetyltransferase KAT8, Protein propionyltransferase KAT8, MYST-1, hMOF

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT8 / MYST1 / MOF antibody (AB72056)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT8 / MYST1 / MOF antibody (AB72056)

IHC image of ab72056 staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab72056, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT8 / MYST1 / MOF antibody (AB72056)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling KAT8 / MYST1 / MOF with ab72056 at 1/1000 (1µg/ml). Detection : DAB.

Unknown

Immunoprecipitation - Anti-KAT8 / MYST1 / MOF antibody (AB72056)

Detection of Human KAT8 / MYST1 / MOF by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab72056 at 3 µg/mg lysate. Subsequent WB detection was done using ab72056 at 1 µg/ml.

All lanes:

Immunoprecipitation - Anti-KAT8 / MYST1 / MOF antibody (ab72056)

Predicted band size: 52 kDa

false

Unknown

Western blot - Anti-KAT8 / MYST1 / MOF antibody (AB72056)

All lanes:

Western blot - Anti-KAT8 / MYST1 / MOF antibody (ab72056) at 0.4 µg/mL

Lane 1:

Whole cell lysate from HeLa cells at 50 µg

Lane 2:

Whole cell lysate from HeLa cells at 15 µg

Lane 3:

Whole cell lysate from HeLa cells at 5 µg

Predicted band size: 52 kDa

Observed band size: 100 kDa,19 kDa,28 kDa,60 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, IP

applications

Immunogen

Synthetic Peptide within Human KAT8 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9H7Z6

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "" }, "Bat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The KAT8 protein also known as MYST1 or MOF functions as a histone acetyltransferase. It acetylates histone H4 at lysine 16 which is critical for chromatin structure and gene expression regulation. The molecular mass of KAT8 is approximately 55 kDa. This protein shows high expression in testis and is also present in other tissues. KAT8 is part of the MYST family of acetyltransferases sharing similarities with other MYST family proteins such as MOF protein.

Biological function summary

KAT8 plays an essential role in gene transcription regulation by forming part of a complex known as the MSL (Male-Specific Lethal) complex. This complex ensures dosage compensation in Drosophila by adjusting gene expression levels. KAT8 is vital for the maintenance of chromatin structure influencing gene activation and silencing across various biological systems. It interacts with several co-factors during transcription regulation further highlighting its functional significance in the epigenetic landscape.

Pathways

KAT8 engages in processes linked to chromatin modification and gene expression. Specifically it plays a significant role in the pathways of chromatin organization and transcriptional regulation by RNA polymerase II. The protein ENL participates in related pathways and interacts with KAT8 during transcription elongation. These interactions highlight KAT8's role in epigenetic regulation affecting multiple genes' transcription states.

KAT8 is associated with cancers and neurodevelopmental disorders. Aberrant expression or mutations in KAT8 have been observed in certain cancers making it a potential target for therapeutic intervention. In neurodevelopmental disorders changes in KAT8 acetyltransferase activity affect neuronal gene expression implicating it in disorders linked to cognitive deficits. Another protein TIP60 shows functional overlap with KAT8 in these conditions suggesting interconnected roles in disease pathogenesis.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Histone acetyltransferase that catalyzes histone H4 acetylation at 'Lys-5'- and 'Lys-8' (H4K5ac and H4K8ac) or 'Lys-16' (H4K16ac), depending on the context (PubMed : 12397079, PubMed : 16227571, PubMed : 16543150, PubMed : 20018852, PubMed : 21217699, PubMed : 22020126, PubMed : 22547026, PubMed : 31794431, PubMed : 33837287). Catalytic component of the MSL histone acetyltransferase complex, a multiprotein complex that mediates the majority of histone H4 acetylation at 'Lys-16' (H4K16ac), an epigenetic mark that prevents chromatin compaction (PubMed : 12397079, PubMed : 16227571, PubMed : 16543150, PubMed : 21217699, PubMed : 22020126, PubMed : 22547026, PubMed : 33657400, PubMed : 33837287). H4K16ac constitutes the only acetylation mark intergenerationally transmitted and regulates key biological processes, such as oogenesis, embryonic stem cell pluripotency, hematopoiesis or glucose metabolism (By similarity). The MSL complex is required for chromosome stability and genome integrity by maintaining homeostatic levels of H4K16ac (PubMed : 33837287). The MSL complex is also involved in gene dosage by promoting up-regulation of genes expressed by the X chromosome (By similarity). X up-regulation is required to compensate for autosomal biallelic expression (By similarity). The MSL complex also participates in gene dosage compensation by promoting expression of Tsix non-coding RNA (By similarity). As part of the NSL histone acetyltransferase complex, catalyzes histone H4 acetylation at 'Lys-5'- and 'Lys-8' (H4K5ac and H4K8ac) at transcription start sites and promotes transcription initiation (PubMed : 20018852, PubMed : 22547026, PubMed : 33657400). The NSL complex also acts as a regulator of gene expression in mitochondria : KAT8 associates with mitochondrial DNA and controls expression of respiratory genes in an acetyltransferase-dependent mechanism (PubMed : 27768893). Also functions as an acetyltransferase for non-histone targets, such as ALKBH5, COX17, IRF3, KDM1A/LSD1, LMNA, PAX7 or TP53/p53 (PubMed : 17189187, PubMed : 19854137, PubMed : 37369679). Acts as an inhibitor of antiviral immunity by acetylating IRF3, preventing IRF3 recruitment to promoters (By similarity). Acts as a regulator of asymmetric division in muscle stem cells by mediating acetylation of PAX7 (By similarity). As part of the NSL complex, acetylates TP53/p53 at 'Lys-120' (PubMed : 17189187, PubMed : 19854137). Acts as a regulator of epithelial-to-mesenchymal transition as part of the NSL complex by mediating acetylation of KDM1A/LSD1 (PubMed : 27292636). The NSL complex is required for nuclear architecture maintenance by mediating acetylation of LMNA (By similarity). Promotes mitochondrial integrity by catalyzing acetylation of COX17 (By similarity). In addition to protein acetyltransferase activity, able to mediate protein propionylation (PubMed : 29321206).

See full target information KAT8

style

left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8190 PubMed40897722

2025

SOX2 drives esophageal squamous carcinoma by reprogramming lipid metabolism and histone acetylation landscape.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Wang,Ruofei Dai,Li Kang,Huan Yang,Zhaosu Chen,Jianzhong He,Lei Shu,Yiting Zhong,Yunfeng Zhang,Zhengyi Hua,Yuanyong Huang,Yuhan Jiang,Jiwen Li,Liyan Xu,Fei Lan,Shu-Hai Lin,Jiemin Wong

Frontiers in oncology 12:842967 PubMed35252011

2022

The Histone Acetyltransferase MOF Regulates SIRT1 Expression to Suppress Renal Cell Carcinoma Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Renbo Guo,Yiran Liang,Benkui Zou,Danyang Li,Zhen Wu,Fei Xie,Xu Zhang,Xiangzhi Li

Frontiers in oncology 11:664403 PubMed34055630

2021

Carnosol Is a Novel Inhibitor of p300 Acetyltransferase in Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Halima Alsamri,Hussain El Hasasna,Bincy Baby,Aysha Alneyadi,Yusra Al Dhaheri,Mohammed Akli Ayoub,Ali H Eid,Ranjit Vijayan,Rabah Iratni

The Journal of biological chemistry 292:11951-11959 PubMed28546430

2017

Cross-talk between the H3K36me3 and H4K16ac histone epigenetic marks in DNA double-strand break repair.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Li,Yinsheng Wang

The FEBS journal 281:3315-24 PubMed24898892

2014

MDM2-MOF-H4K16ac axis contributes to tumorigenesis induced by Notch.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Liu,Zhao-Bin Xing,Shu-Qing Wang,Su Chen,Yan-Kun Liu,Yu-Hui Li,Yu-Feng Li,Ya-Qi Wang,Yang Lu,Wan-Ning Hu,Jing-Hua Zhang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KAT8 / MYST1 / MOF antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT8 / MYST1 / MOF antibody (AB72056)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT8 / MYST1 / MOF antibody (AB72056)

Immunoprecipitation - Anti-KAT8 / MYST1 / MOF antibody (AB72056)

Western blot - Anti-KAT8 / MYST1 / MOF antibody (AB72056)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise