AB113228

Anti-KAT9 / Elp3 antibody

Name: Anti-KAT9 / Elp3 antibody (ab113228) | Abcam
Brand: Abcam Limited
SKU: AB113228
Price: 645 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal KAT9 / Elp3 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Elongator complex protein 3, hELP3, tRNA uridine(34) acetyltransferase, ELP3

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-KAT9 / Elp3 antibody (AB113228)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-KAT9 / Elp3 antibody (AB113228)

ab113228 at 1/100 dilution staining KAT9 / Elp3 in paraformaldehyde-fixed HeLa cells by Immunofluorescence. The lower image is merged with DNA probe.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT9 / Elp3 antibody (AB113228)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT9 / Elp3 antibody (AB113228)

ab113228, at 10µg/ml, staining KAT9 / Elp3 in Formalin-fixed, Paraffin-embedded Human Tonsil tissue by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT9 / Elp3 antibody (AB113228)

ab113228, at 10µg/ml, staining KAT9 / Elp3 in Formalin-fixed, Paraffin-embedded Human Uterus tissue by Immunohistochemistry.

Unknown

Western blot - Anti-KAT9 / Elp3 antibody (AB113228)

7.5% SDS PAGE

All lanes:

Western blot - Anti-KAT9 / Elp3 antibody (ab113228) at 1/2000 dilution

All lanes:

NT2D1 whole cell lysate at 30 µg

Predicted band size: 62 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 89% Tris glycine, 10% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KAT9 also known as Elp3 is an important catalytic component of the elongator complex which is involved in transcriptional elongation. This protein possesses histone acetyltransferase (HAT) activity enabling it to modify histones by acetylating lysine residues a process important for chromatin remodeling and regulation of gene expression. KAT9 is composed of around 55 kDa and is ubiquitously expressed in various tissues reflecting its essential role in diverse cellular processes.

Biological function summary

KAT9/Elp3 is integral in regulating normal cellular growth and differentiation. It functions as part of the elongator complex which consists of six subunits playing a significant role in tRNA modification and mRNA translation elongation. By supporting the translational machinery Elp3 influences cell cycle progression which is critical for proper cellular functions and organism development.

Pathways

The functions of KAT9 hold relevance in the transcriptional and translational regulation pathways. It contributes significantly to the RNA polymerase II-mediated transcription elongation pathway. In addition there is interaction with proteins such as ELP1 and ELP2 with which it forms complexes facilitating various stages of RNA processing and modification. The activity of these complexes impacts cellular responses under stress conditions regulating gene expression for adaptation.

Alterations in KAT9 expression or function are associated with neurodegenerative diseases and cancers. Defects in the elongator complex involving Elp3 relate to familial dysautonomia a disorder affecting the autonomic nervous system. Additionally KAT9 is connected to colorectal cancer where its dysregulation alters transcriptional dynamics potentially contributing to tumor progression. The interplay between Elp3 and other elongator components like Elp1 highlights its significance in maintaining normal cellular homeostasis and preventing pathological states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalytic tRNA acetyltransferase subunit of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine) (PubMed : 29415125). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs (By similarity). May also act as a protein lysine acetyltransferase by mediating acetylation of target proteins; such activity is however unclear in vivo and recent evidences suggest that ELP3 primarily acts as a tRNA acetyltransferase (PubMed : 29415125). Not a major acetyltransferase of tubulin (PubMed : 22153374). Involved in neurogenesis : regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation (PubMed : 19185337). Required for acetylation of GJA1 in the developing cerebral cortex (By similarity).

See full target information ELP3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 10:5492 PubMed31792210

2019

Genome-wide CRISPR screen identifies ELP5 as a determinant of gemcitabine sensitivity in gallbladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sunwang Xu,Ming Zhan,Cen Jiang,Min He,Linhua Yang,Hui Shen,Shuai Huang,Xince Huang,Ruirong Lin,Yongheng Shi,Qiang Liu,Wei Chen,Man Mohan,Jian Wang

Head and neck pathology 13:286-297 PubMed30120721

2018

Identifying Candidate Biomarkers for Pleomorphic Adenoma: A Case-Control Study.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew D Morrison,Linda Jackson-Boeters,Zia A Khan,Michael S Shimizu,Jason H Franklin,Kevin Fung,John H J Yoo,Mark R Darling

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KAT9 / Elp3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-KAT9 / Elp3 antibody (AB113228)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT9 / Elp3 antibody (AB113228)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT9 / Elp3 antibody (AB113228)

Western blot - Anti-KAT9 / Elp3 antibody (AB113228)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise