AB236800

Anti-KAT9 / Elp3 antibody

Name: Anti-KAT9 / Elp3 antibody (ab236800) | Abcam
Brand: Abcam Limited
SKU: AB236800

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(1 Publication)

Rabbit Polyclonal KAT9 / Elp3 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ELP3 aa 1-100.

View Alternative Names

Elongator complex protein 3, hELP3, tRNA uridine(34) acetyltransferase, ELP3

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-KAT9 / Elp3 antibody (AB236800)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KAT9 / Elp3 antibody (AB236800)

4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for KAT9 / Elp3 (green) using ab236800 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488^® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT9 / Elp3 antibody (AB236800)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT9 / Elp3 antibody (AB236800)

Paraffin-embedded human kidney tissue stained for KAT9 / Elp3 using ab236800 at 1/400 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ELP3 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9H9T3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200 - 1/2000", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KAT9 also known as Elp3 is an important catalytic component of the elongator complex which is involved in transcriptional elongation. This protein possesses histone acetyltransferase (HAT) activity enabling it to modify histones by acetylating lysine residues a process important for chromatin remodeling and regulation of gene expression. KAT9 is composed of around 55 kDa and is ubiquitously expressed in various tissues reflecting its essential role in diverse cellular processes.

Biological function summary

KAT9/Elp3 is integral in regulating normal cellular growth and differentiation. It functions as part of the elongator complex which consists of six subunits playing a significant role in tRNA modification and mRNA translation elongation. By supporting the translational machinery Elp3 influences cell cycle progression which is critical for proper cellular functions and organism development.

Pathways

The functions of KAT9 hold relevance in the transcriptional and translational regulation pathways. It contributes significantly to the RNA polymerase II-mediated transcription elongation pathway. In addition there is interaction with proteins such as ELP1 and ELP2 with which it forms complexes facilitating various stages of RNA processing and modification. The activity of these complexes impacts cellular responses under stress conditions regulating gene expression for adaptation.

Alterations in KAT9 expression or function are associated with neurodegenerative diseases and cancers. Defects in the elongator complex involving Elp3 relate to familial dysautonomia a disorder affecting the autonomic nervous system. Additionally KAT9 is connected to colorectal cancer where its dysregulation alters transcriptional dynamics potentially contributing to tumor progression. The interplay between Elp3 and other elongator components like Elp1 highlights its significance in maintaining normal cellular homeostasis and preventing pathological states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Catalytic tRNA acetyltransferase subunit of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine) (PubMed : 29415125). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs (By similarity). May also act as a protein lysine acetyltransferase by mediating acetylation of target proteins; such activity is however unclear in vivo and recent evidences suggest that ELP3 primarily acts as a tRNA acetyltransferase (PubMed : 29415125). Not a major acetyltransferase of tubulin (PubMed : 22153374). Involved in neurogenesis : regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation (PubMed : 19185337). Required for acetylation of GJA1 in the developing cerebral cortex (By similarity).

See full target information ELP3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 12:955510 PubMed36119517

2022

Expression and prognosis analysis of PAQR5 in kidney cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Lu,Hai-Rong Xu,Wei Dong,Hui Dong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KAT9 / Elp3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-KAT9 / Elp3 antibody (AB236800)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT9 / Elp3 antibody (AB236800)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Primary Antibodies

Proteins & Peptides

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise