Anti-KAT9 / Elp3 antibody [EPR18472]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KAT9 / Elp3 antibody [EPR18472] (AB190907)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling KAT9 / Elp3 with ab190907 at 1/2000 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic and nuclear staining on HeLa cells line.The staining pattern is in agreement with the data shown in the following publications : Cell 2009. 136 551-64 (PMID : 19185337) and J Biol Chem. 2002. 277 3047-3052 (PMID : 11714725).

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows : -
-ve control 1 : ab190907 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

• IP

Supplier Data

Immunoprecipitation - Anti-KAT9 / Elp3 antibody [EPR18472] (AB190907)

KAT9 / Elp3 was immunoprecipitated from 1mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with ab190907 at 1/70 dilution.

Lane 1 : MCF7 whole cell lysate 10ug (Input).

Lane 2 : ab190907 IP in MCF7 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab190907 in MCF7 whole cell lysate.

Western blot was performed from the immunoprecipitate using ab190907 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)

Predicted band size: 62 kDa

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• WB

Supplier Data

Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (AB190907)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907) at 1/2000 dilution

Lane 1:

Human fetal brain tissue lysate at 10 µg

Lane 2:

Human fetal heart tissue lysate at 10 µg

Lane 3:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 62 kDa

Observed band size: 62 kDa

false

Exposure time: 3min

• WB

Unknown

Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (AB190907)

All lanes:

Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907) at 1/10000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) cytosolic fraction lysate at 10 µg

Lane 2:

HeLa (Human epithelial cells from cervix adenocarcinoma) nuclear fraction lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/1000 dilution

Predicted band size: 62 kDa

Observed band size: 62 kDa

false

Exposure time: 15s

• WB

Unknown

Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (AB190907)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907) at 1/5000 dilution

Lanes 1 and 7:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 2:

K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate at 10 µg

Lane 3:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 10 µg

Lane 4:

293 (Human epithelial cells from embryonic kidney) whole cell lysate at 10 µg

Lane 5:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 10 µg

Lane 6:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/1000 dilution

Predicted band size: 62 kDa

Observed band size: 62 kDa

false

Exposure time: 5s

• WB

Supplier Data

Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (AB190907)

5% NFDM/TBST : Blocking and diluting buffer.

All lanes:

Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907) at 1/2000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Mouse heart tissue lysate at 10 µg

Lane 3:

Mouse kidney tissue lysate at 10 µg

Lane 4:

Mouse spleen tissue lysate at 10 µg

Lane 5:

Rat brain tissue lysate at 10 µg

Lane 6:

Rat heart tissue lysate at 10 µg

Lane 7:

Rat kidney tissue lysate at 10 µg

Lane 8:

Rat spleen tissue lysate at 10 µg

Lane 9:

C6 (Rat glial tumor cells) whole cell lysate at 10 µg

Lane 10:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 11:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Lane 12:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/1000 dilution

Predicted band size: 62 kDa

Observed band size: 62 kDa

false

Exposure time: 5s