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Rabbit Recombinant Monoclonal KAT9 / Elp3 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

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Images

Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (AB190907), expandable thumbnail
  • Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (AB190907), expandable thumbnail
  • Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (AB190907), expandable thumbnail
  • Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (AB190907), expandable thumbnail
  • Immunoprecipitation - Anti-KAT9 / Elp3 antibody [EPR18472] (AB190907), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Expected
Tested
Expected

Tested
Tested

Species

Human

Dilution info

1/70

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/2000

Notes

-

Species

Rat

Dilution info

1/2000

Notes

-

Species

Human

Dilution info

1/2000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/2000

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Catalytic tRNA acetyltransferase subunit of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine) (PubMed:29415125). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs (By similarity). May also act as a protein lysine acetyltransferase by mediating acetylation of target proteins; such activity is however unclear in vivo and recent evidences suggest that ELP3 primarily acts as a tRNA acetyltransferase (PubMed:29415125). Involved in neurogenesis: regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation (PubMed:19185337). Required for acetylation of GJA1 in the developing cerebral cortex (By similarity).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal KAT9 / Elp3 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR18472

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

KAT9 also known as Elp3 is an important catalytic component of the elongator complex which is involved in transcriptional elongation. This protein possesses histone acetyltransferase (HAT) activity enabling it to modify histones by acetylating lysine residues a process important for chromatin remodeling and regulation of gene expression. KAT9 is composed of around 55 kDa and is ubiquitously expressed in various tissues reflecting its essential role in diverse cellular processes.

Biological function summary

KAT9/Elp3 is integral in regulating normal cellular growth and differentiation. It functions as part of the elongator complex which consists of six subunits playing a significant role in tRNA modification and mRNA translation elongation. By supporting the translational machinery Elp3 influences cell cycle progression which is critical for proper cellular functions and organism development.

Pathways

The functions of KAT9 hold relevance in the transcriptional and translational regulation pathways. It contributes significantly to the RNA polymerase II-mediated transcription elongation pathway. In addition there is interaction with proteins such as ELP1 and ELP2 with which it forms complexes facilitating various stages of RNA processing and modification. The activity of these complexes impacts cellular responses under stress conditions regulating gene expression for adaptation.

Associated diseases and disorders

Alterations in KAT9 expression or function are associated with neurodegenerative diseases and cancers. Defects in the elongator complex involving Elp3 relate to familial dysautonomia a disorder affecting the autonomic nervous system. Additionally KAT9 is connected to colorectal cancer where its dysregulation alters transcriptional dynamics potentially contributing to tumor progression. The interplay between Elp3 and other elongator components like Elp1 highlights its significance in maintaining normal cellular homeostasis and preventing pathological states.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907), expandable thumbnail

    Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907) at 1/5000 dilution

    Lanes 1 and 7: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

    Lane 2: K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate at 10 µg

    Lane 3: Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 10 µg

    Lane 4: 293 (Human epithelial cells from embryonic kidney) whole cell lysate at 10 µg

    Lane 5: HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 10 µg

    Lane 6: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution

    Predicted band size: 62 kDa

    Observed band size: 62 kDa

    Exposure time: 5s

  • Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907), expandable thumbnail

    Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907) at 1/2000 dilution

    Lane 1: Human fetal brain tissue lysate at 10 µg

    Lane 2: Human fetal heart tissue lysate at 10 µg

    Lane 3: Human fetal kidney tissue lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 62 kDa

    Observed band size: 62 kDa

    Exposure time: 3min

  • Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907), expandable thumbnail

    Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)

    5% NFDM/TBST: Blocking and diluting buffer.

    All lanes: Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907) at 1/2000 dilution

    Lane 1: Mouse brain tissue lysate at 10 µg

    Lane 2: Mouse heart tissue lysate at 10 µg

    Lane 3: Mouse kidney tissue lysate at 10 µg

    Lane 4: Mouse spleen tissue lysate at 10 µg

    Lane 5: Rat brain tissue lysate at 10 µg

    Lane 6: Rat heart tissue lysate at 10 µg

    Lane 7: Rat kidney tissue lysate at 10 µg

    Lane 8: Rat spleen tissue lysate at 10 µg

    Lane 9: C6 (Rat glial tumor cells) whole cell lysate at 10 µg

    Lane 10: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Lane 11: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

    Lane 12: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution

    Predicted band size: 62 kDa

    Observed band size: 62 kDa

    Exposure time: 5s

  • Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907), expandable thumbnail

    Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)

    All lanes: Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907) at 1/10000 dilution

    Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) cytosolic fraction lysate at 10 µg

    Lane 2: HeLa (Human epithelial cells from cervix adenocarcinoma) nuclear fraction lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution

    Predicted band size: 62 kDa

    Observed band size: 62 kDa

    Exposure time: 15s

    This data was developed using ab190907, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907), expandable thumbnail

    Immunoprecipitation - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)

    KAT9 / Elp3 was immunoprecipitated from 1mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with ab190907 at 1/70 dilution.

    Lane 1: MCF7 whole cell lysate 10ug (Input).

    Lane 2: ab190907 IP in MCF7 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab190907 in MCF7 whole cell lysate.

    Western blot was performed from the immunoprecipitate using ab190907 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    All lanes: Immunoprecipitation - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)

    Predicted band size: 62 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling KAT9 / Elp3 with ab190907 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic and nuclear staining on HeLa cells line.The staining pattern is in agreement with the data shown in the following publications: Cell 2009. 136, 551-64 (PMID: 19185337) and J Biol Chem. 2002. 277, 3047-3052 (PMID: 11714725).

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:-
    -ve control 1: ab190907 at 1/2000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

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Product protocols

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