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AB251047

Anti-KAT9 / Elp3 antibody [EPR18472] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal KAT9 / Elp3 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Elongator complex protein 3, hELP3, tRNA uridine(34) acetyltransferase, ELP3

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-KAT9 / Elp3 antibody [EPR18472] - BSA and Azide free (AB251047)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KAT9 / Elp3 antibody [EPR18472] - BSA and Azide free (AB251047)

This data was developed using ab190907, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling KAT9 / Elp3 with ab190907 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic and nuclear staining on HeLa cells line.The staining pattern is in agreement with the data shown in the following publications : Cell 2009. 136, 551-64 (PMID : 19185337) and J Biol Chem. 2002. 277, 3047-3052 (PMID : 11714725).

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows : -
-ve control 1 : ab190907 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

Immunoprecipitation - Anti-KAT9 / Elp3 antibody [EPR18472] - BSA and Azide free (AB251047)
  • IP

Supplier Data

Immunoprecipitation - Anti-KAT9 / Elp3 antibody [EPR18472] - BSA and Azide free (AB251047)

This data was developed using ab190907, the same antibody clone in a different buffer formulation.

KAT9 / Elp3 was immunoprecipitated from 1mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with ab190907 at 1/70 dilution.

Lane 1 : MCF7 whole cell lysate 10ug (Input).

Lane 2 : ab190907 IP in MCF7 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab190907 in MCF7 whole cell lysate.

Western blot was performed from the immunoprecipitate using ab190907 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-KAT9 / Elp3 antibody [EPR18472] (<a href='/en-us/products/primary-antibodies/kat9-elp3-antibody-epr18472-ab190907'>ab190907</a>)

Predicted band size: 62 kDa

false

Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] - BSA and Azide free (AB251047)
  • WB

Supplier Data

Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] - BSA and Azide free (AB251047)

This data was developed using ab190907, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (<a href='/en-us/products/primary-antibodies/kat9-elp3-antibody-epr18472-ab190907'>ab190907</a>) at 1/2000 dilution

Lane 1:

Human fetal brain tissue lysate at 10 µg

Lane 2:

Human fetal heart tissue lysate at 10 µg

Lane 3:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 62 kDa

Observed band size: 62 kDa

false

Exposure time: 3min

Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] - BSA and Azide free (AB251047)
  • WB

Unknown

Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] - BSA and Azide free (AB251047)

This data was developed using ab190907, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (<a href='/en-us/products/primary-antibodies/kat9-elp3-antibody-epr18472-ab190907'>ab190907</a>) at 1/10000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) cytosolic fraction lysate at 10 µg

Lane 2:

HeLa (Human epithelial cells from cervix adenocarcinoma) nuclear fraction lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/1000 dilution

Predicted band size: 62 kDa

Observed band size: 62 kDa

false

Exposure time: 15s

Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] - BSA and Azide free (AB251047)
  • WB

Unknown

Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] - BSA and Azide free (AB251047)

This data was developed using ab190907, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (<a href='/en-us/products/primary-antibodies/kat9-elp3-antibody-epr18472-ab190907'>ab190907</a>) at 1/5000 dilution

Lanes 1 and 7:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 2:

K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate at 10 µg

Lane 3:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 10 µg

Lane 4:

293 (Human epithelial cells from embryonic kidney) whole cell lysate at 10 µg

Lane 5:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 10 µg

Lane 6:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/1000 dilution

Predicted band size: 62 kDa

Observed band size: 62 kDa

false

Exposure time: 5s

Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] - BSA and Azide free (AB251047)
  • WB

Supplier Data

Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] - BSA and Azide free (AB251047)

This data was developed using ab190907, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KAT9 / Elp3 antibody [EPR18472] (<a href='/en-us/products/primary-antibodies/kat9-elp3-antibody-epr18472-ab190907'>ab190907</a>) at 1/2000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Mouse heart tissue lysate at 10 µg

Lane 3:

Mouse kidney tissue lysate at 10 µg

Lane 4:

Mouse spleen tissue lysate at 10 µg

Lane 5:

Rat brain tissue lysate at 10 µg

Lane 6:

Rat heart tissue lysate at 10 µg

Lane 7:

Rat kidney tissue lysate at 10 µg

Lane 8:

Rat spleen tissue lysate at 10 µg

Lane 9:

C6 (Rat glial tumor cells) whole cell lysate at 10 µg

Lane 10:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 11:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Lane 12:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/1000 dilution

Predicted band size: 62 kDa

Observed band size: 62 kDa

false

Exposure time: 5s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18472

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab251047 is the carrier-free version of ab190907.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KAT9 also known as Elp3 is an important catalytic component of the elongator complex which is involved in transcriptional elongation. This protein possesses histone acetyltransferase (HAT) activity enabling it to modify histones by acetylating lysine residues a process important for chromatin remodeling and regulation of gene expression. KAT9 is composed of around 55 kDa and is ubiquitously expressed in various tissues reflecting its essential role in diverse cellular processes.
Biological function summary

KAT9/Elp3 is integral in regulating normal cellular growth and differentiation. It functions as part of the elongator complex which consists of six subunits playing a significant role in tRNA modification and mRNA translation elongation. By supporting the translational machinery Elp3 influences cell cycle progression which is critical for proper cellular functions and organism development.

Pathways

The functions of KAT9 hold relevance in the transcriptional and translational regulation pathways. It contributes significantly to the RNA polymerase II-mediated transcription elongation pathway. In addition there is interaction with proteins such as ELP1 and ELP2 with which it forms complexes facilitating various stages of RNA processing and modification. The activity of these complexes impacts cellular responses under stress conditions regulating gene expression for adaptation.

Alterations in KAT9 expression or function are associated with neurodegenerative diseases and cancers. Defects in the elongator complex involving Elp3 relate to familial dysautonomia a disorder affecting the autonomic nervous system. Additionally KAT9 is connected to colorectal cancer where its dysregulation alters transcriptional dynamics potentially contributing to tumor progression. The interplay between Elp3 and other elongator components like Elp1 highlights its significance in maintaining normal cellular homeostasis and preventing pathological states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalytic tRNA acetyltransferase subunit of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine) (PubMed : 29415125). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs (By similarity). May also act as a protein lysine acetyltransferase by mediating acetylation of target proteins; such activity is however unclear in vivo and recent evidences suggest that ELP3 primarily acts as a tRNA acetyltransferase (PubMed : 29415125). Involved in neurogenesis : regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation (PubMed : 19185337). Required for acetylation of GJA1 in the developing cerebral cortex (By similarity).
See full target information ELP3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 21:454-462 PubMed31746378

2019

Blueberry anthocyanin‑enriched extract ameliorates transverse aortic constriction‑induced myocardial dysfunction via the DDAH1/ADMA/NO signaling pathway in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Weiqing Hu,Wenyue Wang,Qing Ma,Tao Liu,Jiefeng Zhang,Jicun Zhang
View all publications
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