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AB184829

Anti-KATNAL2 antibody

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(1 Publication)

Rabbit Polyclonal KATNAL2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human KATNAL2 aa 150-300.

View Alternative Names

Katanin p60 ATPase-containing subunit A-like 2, Katanin p60 subunit A-like 2, p60 katanin-like 2, KATNAL2

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KATNAL2 antibody (AB184829)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KATNAL2 antibody (AB184829)

Immunohistochemical analysis of human fallopian tube tissue labeling KATNAL2 in the cytoplasm of glandular cells with ab184829 at a 1/20 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KATNAL2 antibody (AB184829)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KATNAL2 antibody (AB184829)

Immunohistochemical analysis of human skeletal muscle tissue labeling KATNAL2 in the cytoplasm of myocytes with ab184829 at a 1/20 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KATNAL2 antibody (AB184829)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KATNAL2 antibody (AB184829)

Immunohistochemical analysis of human testis tissue labeling KATNAL2 in the cytoplasm and membrane of seminiferous ducts with ab184829 at a 1/20 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-KATNAL2 antibody (AB184829)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KATNAL2 antibody (AB184829)

Immunofluorescence analysis of hU-2 OS (human bone osteosarcoma epithelial cell line) whole cells labeling KATNAL2 in the nucleoplasm and intermediate filaments with ab184829 at 2 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KATNAL2 antibody (AB184829)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KATNAL2 antibody (AB184829)

Immunohistochemical analysis of human thyroid gland labeling KATNAL2 in the cytoplasm of glandular cells with ab184829 at a 1/20 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KATNAL2 antibody (AB184829)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KATNAL2 antibody (AB184829)

Immunohistochemical analysis of human cerebral cortex tissue labeling KATNAL2 in the cytoplasm of neurons with ab184829 at a 1/20 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-KATNAL2 antibody (AB184829)
  • WB

Supplier Data

Western blot - Anti-KATNAL2 antibody (AB184829)

Corresponding KATNAL2 RNA-seq data are presented for the same cell lines. Loading control : Anti-GAPDH.

All lanes:

Western blot - Anti-KATNAL2 antibody (ab184829) at 0.4 µg/mL

Lane 1:

A549 (human lung carcinoma cell line) whole cell lysate

Lane 2:

Caco-2 (human colorectal adenocarcinoma cell line) whole cell lysate

Predicted band size: 61 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human KATNAL2 aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

Q8IYT4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KATNAL2 also known by its official full name Katanin catalytic subunit A-like 2 is a protein involved in microtubule severing. It possesses a molecular mass of approximately 63 kDa. KATNAL2 is expressed in various tissues throughout the body with notable expression in the brain and reproductive organs. This protein is important for the regulation of the cytoskeleton through the disassembly of microtubules contributing to cellular structure maintenance.
Biological function summary

KATNAL2 plays a role in cellular processes like mitosis and neuronal development. As part of a larger protein complex it interacts with other proteins to facilitate its microtubule severing activity. This action helps in remodeling the microtubule network which is essential for cellular dynamics and morphological changes during cell division and differentiation. The precise assembly and disassembly controlled by KATNAL2 are important for ensuring normal cell function and development.

Pathways

KATNAL2 associates with the microtubule dynamics pathway and is involved in mitotic spindle organization. It interacts with proteins such as katanin p60 which assists in severing microtubules. This interaction allows for the correct segregation of chromosomes during cell division. Additionally KATNAL2 may influence signaling pathways related to cytoskeletal organization demonstrating its importance in maintaining cellular integrity.

Changes in KATNAL2 function link to neurodevelopmental disorders and male infertility. Abnormal expression levels of KATNAL2 have implications in conditions affecting neuronal development where its role alongside proteins such as the tubulin family becomes apparent. Disruption in these pathways related to KATNAL2 can lead to disorders characterized by improper neuronal connections or alterations in testicular structure leading to infertility. Understanding KATNAL2's interaction with related proteins sheds light on its impact on these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Severs microtubules in vitro in an ATP-dependent manner. This activity may promote rapid reorganization of cellular microtubule arrays.
See full target information KATNAL2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Gut and liver 7:739-46 PubMed24312717

2013

Expression of heat shock protein 70 modulates the chemoresponsiveness of pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jong Jin Hyun,Hong Sik Lee,Bora Keum,Yeon Seok Seo,Yoon Tae Jeen,Hoon Jai Chun,Soon Ho Um,Chang Duck Kim
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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