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AB222971

Anti-KBTB6/KBTBD6 antibody

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(1 Publication)

Rabbit Polyclonal KBTB6/KBTBD6 antibody. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human KBTBD6 aa 1-150.

View Alternative Names

Kelch repeat and BTB domain-containing protein 6, KBTBD6

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-KBTB6/KBTBD6 antibody (AB222971)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KBTB6/KBTBD6 antibody (AB222971)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for KBTB6/KBTBD6 (green) using ab222971 at 1/100 dilution in ICC/IF. Secondary : Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTB6/KBTBD6 antibody (AB222971)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTB6/KBTBD6 antibody (AB222971)

Paraffin-embedded human liver tissue stained for KBTB6/KBTBD6 using ab222971 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-KBTB6/KBTBD6 antibody (AB222971)
  • WB

Supplier Data

Western blot - Anti-KBTB6/KBTBD6 antibody (AB222971)

All lanes:

Western blot - Anti-KBTB6/KBTBD6 antibody (ab222971) at 1/500 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

Mouse liver tissue lysate

Lane 3:

Mouse kidney tissue lysate

Lane 4:

Mouse brain tissue lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG at 1/50000 dilution

Predicted band size: 76 kDa

Observed band size: 50 kDa,77 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human KBTBD6 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q86V97

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KBTB6 also known as KBTBD6 is a protein that functions as an E3 ubiquitin ligase specifically targeting proteins for ubiquitination and subsequent proteasomal degradation. The protein has a molecular mass of approximately 64 kDa. KBTB6 is expressed in various tissues with noticeable levels in the liver and muscle tissues. It interacts with Cullin-RING ubiquitin ligase complexes facilitating the transfer of ubiquitin molecules to substrates which marks them for degradation.
Biological function summary

The E3 ligase activity of KBTB6 regulates protein homeostasis by degrading unnecessary or damaged proteins. It plays an essential role in several cellular processes including cell cycle regulation signal transduction and cellular differentiation. KBTB6 works as part of a larger complex with other proteins like cullins and RING box proteins to perform its function effectively. These complexes ensure the specificity of substrate identification and ubiquitination.

Pathways

KBTB6 influences various cellular pathways by regulating protein stability. It is involved in the Wnt signaling pathway and also links to the p53 pathway by modulating the levels of critical regulatory proteins within these pathways. In these roles KBTB6 interacts with proteins like beta-catenin in the Wnt pathway and Mdm2 in the p53 pathway which are important for the regulation of cellular proliferation and apoptosis.

Disruptions in KBTB6 function can relate to cancer and muscular disorders. Its role in regulating the degradation of proteins involved in cell cycle and apoptosis makes it significant in tumorigenesis where alterations can lead to uncontrolled cell growth. Also abnormalities in KBTB6 activity are associated with certain muscle diseases due to its expression in muscle tissues. In these conditions it may interact with other proteins such as p53 or beta-catenin which play roles in cell regulation and signal transduction processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

As part of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex functions as a substrate adapter for the RAC1 guanine exchange factor (GEF) TIAM1, mediating its 'Lys-48' ubiquitination and proteasomal degradation (PubMed : 25684205). By controlling this ubiquitination, regulates RAC1 signal transduction and downstream biological processes including the organization of the cytoskeleton, cell migration and cell proliferation (PubMed : 25684205). Ubiquitination of TIAM1 requires the membrane-associated protein GABARAP which may restrict locally the activity of the complex (PubMed : 25684205).
See full target information KBTBD6
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

mAbs 8:454-67 PubMed26854859

2016

Inhibition of CD73 AMP hydrolysis by a therapeutic antibody with a dual, non-competitive mechanism of action.

Applications

Unspecified application

Species

Unspecified reactive species

James C Geoghegan,Gundo Diedrich,Xiaojun Lu,Kim Rosenthal,Kris F Sachsenmeier,Herren Wu,William F Dall'Acqua,Melissa M Damschroder
View all publications
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