AB169551

Anti-KBTBD11 antibody [EPR10056(2)(B)]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal KBTBD11 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CMLAP, KIAA0711, KLHDC7C, KBTBD11, Kelch repeat and BTB domain-containing protein 11, Chronic myelogenous leukemia-associated protein, Kelch domain-containing protein 7B

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD11 antibody [EPR10056(2)(B)] (AB169551)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD11 antibody [EPR10056(2)(B)] (AB169551)

Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue labeling KBTBD11 with ab169551 antibody at 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD11 antibody [EPR10056(2)(B)] (AB169551)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD11 antibody [EPR10056(2)(B)] (AB169551)

ab169551 showing +ve staining in Human normal tonsil tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD11 antibody [EPR10056(2)(B)] (AB169551)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD11 antibody [EPR10056(2)(B)] (AB169551)

ab169551 showing +ve staining in Human prostate hyperplasia tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD11 antibody [EPR10056(2)(B)] (AB169551)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD11 antibody [EPR10056(2)(B)] (AB169551)

ab169551 showing +ve staining in Human normal kidney tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD11 antibody [EPR10056(2)(B)] (AB169551)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD11 antibody [EPR10056(2)(B)] (AB169551)

Immunohistochemical analysis of paraffin embedded Human uterus tissue labeling KBTBD11 with ab169551 antibody at 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-KBTBD11 antibody [EPR10056(2)(B)] (AB169551)

IP

Unknown

Immunoprecipitation - Anti-KBTBD11 antibody [EPR10056(2)(B)] (AB169551)

Immunoprecipitation. ab169551 at 1/1000 staining KBTBD11 in fetal brain lysate immunoprecipitated using ab169551 at 1/10. Predicted band size : 66 kDa

All lanes:

Immunoprecipitation - Anti-KBTBD11 antibody [EPR10056(2)(B)] (ab169551)

Predicted band size: 66 kDa

false

Western blot - Anti-KBTBD11 antibody [EPR10056(2)(B)] (AB169551)

WB

Unknown

Western blot - Anti-KBTBD11 antibody [EPR10056(2)(B)] (AB169551)

All lanes:

Western blot - Anti-KBTBD11 antibody [EPR10056(2)(B)] (ab169551) at 1/1000 dilution

Lane 1:

Fetal brain lysate at 10 µg

Lane 2:

293T lysate at 10 µg

Lane 3:

HT-29 lysate at 10 µg

Lane 4:

Caco-2 lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 66 kDa

Observed band size: 66 kDa

false

Carrier free

Anti-KBTBD11 antibody [EPR10056(2)(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10056(2)(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB, IHC-P

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/50", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KBTBD11 also known as Kelch Repeat and BTB (POZ) Domain-Containing Protein 11 is a protein with a molecular mass of approximately 70 kDa. This protein is part of the BTB-Kelch family which are characterized by a BTB protein-protein interaction domain and Kelch repeats that form a beta-propeller structure. KBTBD11 is expressed in various tissues with higher expression seen in the brain and testis indicating a role in neural activities and reproductive processes.

Biological function summary

KBTBD11 participates in the ubiquitin-proteasome system where it acts as a substrate adaptor for Cullin-RING E3 ligases (CRLs). By facilitating the ubiquitination of target proteins KBTBD11 regulates protein turnover and stability. It does not function independently and is part of a larger CRL complex that includes various other scaffolding and substrate recognition proteins. Through these interactions KBTBD11 impacts cellular processes such as signal transduction and cell cycle progression.

Pathways

KBTBD11 is involved in the ubiquitination and proteasome degradation pathways critical for maintaining proteostasis within cells. It shares functional relationships with other components like Cullin-3 which form an E3 ligase complex important for tagging damaged or unneeded proteins for degradation. This regulatory mechanism plays a significant role in controlling signal transduction pathways including cell growth and apoptotic processes in which protein degradation must be swiftly and accurately regulated.

KBTBD11 links to neurodegenerative diseases and cancers due to its role in protein degradation. Misregulation of its function can lead to an accumulation of ubiquitinated proteins which is a hallmark of neurodegenerative conditions such as Alzheimer’s disease. It may also be involved in cancer through pathways associated with cell cycle control where its malfunction can contribute to uncontrolled cell proliferation. In these contexts proteins like p53 are often critical in mediating the pathways impacted by KBTBD11 dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information KBTBD11

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncogene 44:4324-4337 PubMed40983636

2025

KBTBD11 loss promotes AKT hyperactivation and therapeutic vulnerability in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Haoyue Sheng,Guohai Shi,Yawen Lu,Shengfeng Zheng,Weijie Gu,Dan Xia,Haojie Huang,Dingwei Ye

Frontiers in pharmacology 12:718675 PubMed34646134

2021

Upregulation of Thymidylate Synthase Induces Pemetrexed Resistance in Malignant Pleural Mesothelioma.

Applications

Unspecified application

Species

Unspecified reactive species

Yuzo Sato,Masaru Tomita,Tomoyoshi Soga,Atsushi Ochiai,Hideki Makinoshima

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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