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Rabbit Recombinant Monoclonal KBTBD11 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD11 antibody [EPR10056(2)(B)] (AB169551), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD11 antibody [EPR10056(2)(B)] (AB169551), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD11 antibody [EPR10056(2)(B)] (AB169551), expandable thumbnail
  • Immunoprecipitation - Anti-KBTBD11 antibody [EPR10056(2)(B)] (AB169551), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD11 antibody [EPR10056(2)(B)] (AB169551), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWBICC/IF
Human
Tested
Tested
Not recommended
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/50
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/5000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Alternative names

CMLAP, KIAA0711, KLHDC7C, KBTBD11, Kelch repeat and BTB domain-containing protein 11, Chronic myelogenous leukemia-associated protein, Kelch domain-containing protein 7B

Recommended products

Rabbit Recombinant Monoclonal KBTBD11 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR10056(2)(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

KBTBD11 also known as Kelch Repeat and BTB (POZ) Domain-Containing Protein 11 is a protein with a molecular mass of approximately 70 kDa. This protein is part of the BTB-Kelch family which are characterized by a BTB protein-protein interaction domain and Kelch repeats that form a beta-propeller structure. KBTBD11 is expressed in various tissues with higher expression seen in the brain and testis indicating a role in neural activities and reproductive processes.

Biological function summary

KBTBD11 participates in the ubiquitin-proteasome system where it acts as a substrate adaptor for Cullin-RING E3 ligases (CRLs). By facilitating the ubiquitination of target proteins KBTBD11 regulates protein turnover and stability. It does not function independently and is part of a larger CRL complex that includes various other scaffolding and substrate recognition proteins. Through these interactions KBTBD11 impacts cellular processes such as signal transduction and cell cycle progression.

Pathways

KBTBD11 is involved in the ubiquitination and proteasome degradation pathways critical for maintaining proteostasis within cells. It shares functional relationships with other components like Cullin-3 which form an E3 ligase complex important for tagging damaged or unneeded proteins for degradation. This regulatory mechanism plays a significant role in controlling signal transduction pathways including cell growth and apoptotic processes in which protein degradation must be swiftly and accurately regulated.

Associated diseases and disorders

KBTBD11 links to neurodegenerative diseases and cancers due to its role in protein degradation. Misregulation of its function can lead to an accumulation of ubiquitinated proteins which is a hallmark of neurodegenerative conditions such as Alzheimer’s disease. It may also be involved in cancer through pathways associated with cell cycle control where its malfunction can contribute to uncontrolled cell proliferation. In these contexts proteins like p53 are often critical in mediating the pathways impacted by KBTBD11 dysfunction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD11 antibody [EPR10056(2)(B)] (ab169551), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD11 antibody [EPR10056(2)(B)] (ab169551)

    ab169551 showing +ve staining in Human normal kidney tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD11 antibody [EPR10056(2)(B)] (ab169551), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD11 antibody [EPR10056(2)(B)] (ab169551)

    ab169551 showing +ve staining in Human prostate hyperplasia tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD11 antibody [EPR10056(2)(B)] (ab169551), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD11 antibody [EPR10056(2)(B)] (ab169551)

    ab169551 showing +ve staining in Human normal tonsil tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-KBTBD11 antibody [EPR10056(2)(B)] (ab169551), expandable thumbnail

    Immunoprecipitation - Anti-KBTBD11 antibody [EPR10056(2)(B)] (ab169551)

    Immunoprecipitation. ab169551 at 1/1000 staining KBTBD11 in fetal brain lysate immunoprecipitated using ab169551 at 1/10.
    Predicted band size : 66 kDa

    All lanes: Immunoprecipitation - Anti-KBTBD11 antibody [EPR10056(2)(B)] (ab169551)

    Predicted band size: 66 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD11 antibody [EPR10056(2)(B)] (ab169551), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD11 antibody [EPR10056(2)(B)] (ab169551)

    Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue labeling KBTBD11 with ab169551 antibody at 1/100.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD11 antibody [EPR10056(2)(B)] (ab169551), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD11 antibody [EPR10056(2)(B)] (ab169551)

    Immunohistochemical analysis of paraffin embedded Human uterus tissue labeling KBTBD11 with ab169551 antibody at 1/100.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-KBTBD11 antibody [EPR10056(2)(B)] (ab169551), expandable thumbnail

    Western blot - Anti-KBTBD11 antibody [EPR10056(2)(B)] (ab169551)

    All lanes: Western blot - Anti-KBTBD11 antibody [EPR10056(2)(B)] (ab169551) at 1/1000 dilution

    Lane 1: Fetal brain lysate at 10 µg

    Lane 2: 293T lysate at 10 µg

    Lane 3: HT-29 lysate at 10 µg

    Lane 4: Caco-2 lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 66 kDa

    Observed band size: 66 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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