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AB230126

Anti-KBTBD7 antibody

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(3 Publications)

Rabbit Polyclonal KBTBD7 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KBTBD7 aa 450 to C-terminus.

View Alternative Names

Kelch repeat and BTB domain-containing protein 7, KBTBD7

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD7 antibody (AB230126)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD7 antibody (AB230126)

Paraffin-embedded human brain tissue stained for KBTBD7 with ab230126 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD7 antibody (AB230126)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTBD7 antibody (AB230126)

Paraffin-embedded human testis tissue stained for KBTBD7 with ab230126 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-KBTBD7 antibody (AB230126)
  • WB

Supplier Data

Western blot - Anti-KBTBD7 antibody (AB230126)

All lanes:

Western blot - Anti-KBTBD7 antibody (ab230126) at 1/1000 dilution

Lane 1:

HL-60 (Human promyelocytic leukemia cell line) whole cell lysate

Lane 2:

A549 (Human lung carcinoma cell line) whole cell lysate

Lane 3:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Secondary

All lanes:

Goat polyclonal to Rabbit IgG at 1/10000 dilution

Predicted band size: 77 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human KBTBD7 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8WVZ9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KBTBD7 also known as Kelch repeat and BTB domain-containing protein 7 plays a significant role in cellular processes. It exhibits a molecular mass of approximately 67 kDa. KBTBD7 expresses across various tissues with notable levels in the brain and kidney. The protein contains a BTB/POZ domain and kelch repeats which are essential for its interaction with other proteins and participation in ubiquitin-mediated proteolysis.
Biological function summary

KBTBD7 participates in cellular regulation by mediating protein degradation. It forms part of a complex involved in ubiquitin-protein ligase activity facilitating the tagging of proteins for degradation. The protein's activity is important in maintaining protein homeostasis and removing damaged or unnecessary proteins from the cell. Its involvement in these processes emphasizes its role in cellular quality control.

Pathways

KBTBD7 influences the ubiquitin-proteasome pathway important for protein turnover. This pathway regulates the degradation of key proteins affecting various cellular activities. KBTBD7 interacts with proteins such as CUL3 where it acts as an adaptor in the cullin-RING E3 ubiquitin ligase (CRL) complexes. Through these interactions it indirectly influences cellular events governed by ubiquitin-mediated processes.

KBTBD7 has associations with neurodegenerative diseases and cancer. Dysregulation in the ubiquitin-proteasome pathway involving KBTBD7 contributes to the accumulation of misfolded proteins affecting diseases like Alzheimer's. In cancer improper KBTBD7 activity might lead to unrestrained cell proliferation due to disrupted protein degradation cycles. Connections with proteins like Cyclins highlight the impact of its regulatory malfunction in pathological states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

As part of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex functions as a substrate adapter for the RAC1 guanine exchange factor (GEF) TIAM1, mediating its 'Lys-48' ubiquitination and proteasomal degradation (PubMed : 25684205). By controlling this ubiquitination, regulates RAC1 signal transduction and downstream biological processes including the organization of the cytoskeleton, cell migration and cell proliferation (PubMed : 25684205). Ubiquitination of TIAM1 requires the membrane-associated protein GABARAP which may restrict locally the activity of the complex (PubMed : 25684205).
See full target information KBTBD7
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 58:102544 PubMed36427397

2022

CPT1A promotes anoikis resistance in esophageal squamous cell carcinoma via redox homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Tian,Yunxin Lu,Jinfei Lin,Miao Chen,Huijuan Qiu,Wancui Zhu,Haohui Sun,Jinsheng Huang,Han Yang,Wuguo Deng

Molecular cell 82:585-597.e11 PubMed35120648

2022

Co-adaptor driven assembly of a CUL3 E3 ligase complex.

Applications

Unspecified application

Species

Unspecified reactive species

David Akopian,Colleen A McGourty,Michael Rapé

Medical science monitor : international medical jo 26:e920849 PubMed32332694

2020

Sevoflurane Postconditioning Reduces Hypoxia-Reoxygenation Injury in H9C2 Embryonic Rat Cardiomyocytes and Targets the STRADA Gene by Upregulating microRNA-107.

Applications

Unspecified application

Species

Unspecified reactive species

Qun Jiang,Shan Gu
View all publications
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