Goat Polyclonal KCC1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SLC12A4 aa 950-1050.
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Publications
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- Nature communications 9:21672018Cotransporter-mediated water transport underlying cerebrospinal fluid formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnnette B Steffensen,Eva K Oernbo,Anca Stoica,Niklas J Gerkau,Dagne Barbuskaite,Katerina Tritsaris,Christine R Rose,Nanna MacAulayPubMed 29867199
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SLC12A4 aa 950-1050. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UP95
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Sheep | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Sheep, Rabbit, Horse, Cow, Dog, Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.30000-1.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Sheep, Rabbit, Horse, Cow, Dog, Pig | Dilution info - | Notes - |
Target data
Function
Mediates electroneutral potassium-chloride cotransport when activated by cell swelling (PubMed:35759661). May contribute to cell volume homeostasis in single cells (PubMed:10913127, PubMed:34031912). May be involved in the regulation of basolateral Cl(-) exit in NaCl absorbing epithelia (By similarity). Isoform 4. No transporter activity.
Alternative names
KCC1, SLC12A4, Solute carrier family 12 member 4, Electroneutral potassium-chloride cotransporter 1, Erythroid K-Cl cotransporter 1, hKCC1
Recommended products
Goat Polyclonal KCC1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SLC12A4 aa 950-1050.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SLC12A4 aa 950-1050. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UP95
- Purification technique
- Affinity purification Protein G
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
KCC1 also known as SLC12A4 is a member of the K-Cl cotransporter family. It mechanically transports potassium (K+) and chloride (Cl-) ions across the cell membrane. This transport occurs through an electroneutral process that plays an important role in maintaining cellular ionic homeostasis. KCC1 is expressed widely in many tissues including the kidney brain and heart. The molecular mass of KCC1 is approximately 120 kDa. Its expression in diverse tissues indicates its importance in various physiological processes.
- Biological function summary
KCC1 regulates the cell volume and chloride ion concentration. It functions as a part of a complex that balances osmotic pressure within cells influencing processes like neuronal signaling and cell proliferation. In neurons KCC1 helps maintain the resting membrane potential which is essential for proper signaling. Its activity in the kidney regulates electrolyte balance contributing to normal renal function. The role of KCC1 in cell volume regulation highlights its involvement in fundamental cellular processes.
- Pathways
KCC1 plays roles in the WNK-SPAK/OSR1 pathway an important element in ion homeostasis. This pathway influences the regulation of electrolyte transport in epithelial cells. KCC1 interacts with proteins like WNK kinases and SPAK which modulate its activity in response to changes in cellular hydration status. The crosstalk and regulation within these pathways ensure the tight control of ion transport necessary for proper cellular environment maintenance.
- Associated diseases and disorders
KCC1 has links to hypertension and neurological disorders. Its dysregulation impacts blood pressure through its effects on renal electrolyte handling suggesting a connection with hypertension. Additionally KCC1's role in neuronal ion balance indicates potential involvement in epilepsy. In epilepsy altered KCC1 function can influence the excitability of neurons potentially relating to seizure activity. Interactions with proteins such as WNK1 highlight the role of KCC1 in these pathological conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCC1 antibody (ab115607)
ab115607, at 5μg/ml, staining KCC1 in Formalin-fixed, Paraffin-embedded Human Placenta tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot - Anti-KCC1 antibody (ab115607)
All lanes: Western blot - Anti-KCC1 antibody (ab115607) at 0.3 µg/mL
All lanes: Human heart lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 121 kDa
Observed band size: 150 kDa
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