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AB115607

Anti-KCC1 antibody

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(1 Publication)

Goat Polyclonal KCC1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SLC12A4 aa 950-1050.

View Alternative Names

KCC1, SLC12A4, Solute carrier family 12 member 4, Electroneutral potassium-chloride cotransporter 1, Erythroid K-Cl cotransporter 1, hKCC1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCC1 antibody (AB115607)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCC1 antibody (AB115607)

ab115607, at 5μg/ml, staining KCC1 in Formalin-fixed, Paraffin-embedded Human Placenta tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Western blot - Anti-KCC1 antibody (AB115607)
  • WB

Unknown

Western blot - Anti-KCC1 antibody (AB115607)

All lanes:

Western blot - Anti-KCC1 antibody (ab115607) at 0.3 µg/mL

All lanes:

Human heart lysate at 35 µg

Predicted band size: 121 kDa

Observed band size: 150 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human SLC12A4 aa 950-1050. The exact immunogen used to generate this antibody is proprietary information.

Q9UP95

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KCC1 also known as SLC12A4 is a member of the K-Cl cotransporter family. It mechanically transports potassium (K+) and chloride (Cl-) ions across the cell membrane. This transport occurs through an electroneutral process that plays an important role in maintaining cellular ionic homeostasis. KCC1 is expressed widely in many tissues including the kidney brain and heart. The molecular mass of KCC1 is approximately 120 kDa. Its expression in diverse tissues indicates its importance in various physiological processes.
Biological function summary

KCC1 regulates the cell volume and chloride ion concentration. It functions as a part of a complex that balances osmotic pressure within cells influencing processes like neuronal signaling and cell proliferation. In neurons KCC1 helps maintain the resting membrane potential which is essential for proper signaling. Its activity in the kidney regulates electrolyte balance contributing to normal renal function. The role of KCC1 in cell volume regulation highlights its involvement in fundamental cellular processes.

Pathways

KCC1 plays roles in the WNK-SPAK/OSR1 pathway an important element in ion homeostasis. This pathway influences the regulation of electrolyte transport in epithelial cells. KCC1 interacts with proteins like WNK kinases and SPAK which modulate its activity in response to changes in cellular hydration status. The crosstalk and regulation within these pathways ensure the tight control of ion transport necessary for proper cellular environment maintenance.

KCC1 has links to hypertension and neurological disorders. Its dysregulation impacts blood pressure through its effects on renal electrolyte handling suggesting a connection with hypertension. Additionally KCC1's role in neuronal ion balance indicates potential involvement in epilepsy. In epilepsy altered KCC1 function can influence the excitability of neurons potentially relating to seizure activity. Interactions with proteins such as WNK1 highlight the role of KCC1 in these pathological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mediates electroneutral potassium-chloride cotransport when activated by cell swelling (PubMed : 35759661). May contribute to cell volume homeostasis in single cells (PubMed : 10913127, PubMed : 34031912). May be involved in the regulation of basolateral Cl(-) exit in NaCl absorbing epithelia (By similarity).. Isoform 4. No transporter activity.
See full target information SLC12A4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 9:2167 PubMed29867199

2018

Cotransporter-mediated water transport underlying cerebrospinal fluid formation.

Applications

Unspecified application

Species

Unspecified reactive species

Annette B Steffensen,Eva K Oernbo,Anca Stoica,Niklas J Gerkau,Dagne Barbuskaite,Katerina Tritsaris,Christine R Rose,Nanna MacAulay
View all publications
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Product promise

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