JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB283588

Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal KCC2 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.

View Alternative Names

KCC2, KIAA1176, SLC12A5, Solute carrier family 12 member 5, Electroneutral potassium-chloride cotransporter 2, K-Cl cotransporter 2, Neuronal K-Cl cotransporter, hKCC2

18 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)

This data was developed using ab259969, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labelling KCC2 with ab259969 at 1/5000 dilution (0.111 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cerebrum.The section was incubated with ab259969 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunocytochemistry/ Immunofluorescence - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)

This data was developed using ab259969, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cells labelling KCC2 with ab259969 at 1/200 dilution (2.775 μg/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 μg/ml)(Green). ab11267 Anti-MAP2 mouse monoclonal antibody at 1/500 dilution (4 μg/ml) followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution (2 μg/ml) was used to counterstain tubulin (Red). The Nuclear counterstain was DAPI (Blue).

Confocal image showing positive staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 μg/ml).

Negative control 1 : ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution (2 μg/ml).

Negative control 2 : ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 μg/ml).

Immunohistochemistry (Frozen sections) - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)

This data was developed using ab259969, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver(fresh) tissue labeling KCC2 with ab259969 at 1/100 dilution (5.55 μg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 μg/ml)(Green). The nuclear counterstain was DAPI (Blue). No staining on mouse liver.

Negative control : liver (PMID : 17715129) is observed.

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 μg/ml).

Flow Cytometry (Intracellular) - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)

Flow cytometric (intracellular) analysis of 4% paraformaldehyde-fixed Mouse primary neuron cells labelling KCC2 with ab259969 at 1/500 dilution (Right) compared with Isotype control Rabbit monoclonal IgG (ab172730) (Left). Goat Anti-Rabbit IgG Alexa Fluor® 488 (ab150081) was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)

This data was developed using ab259969, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling KCC2 with ab259969 at 1/5000 dilution (0.111 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebrum. The section was incubated with ab259969 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Frozen sections) - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)

This data was developed using ab259969, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat liver (fresh) tissue labeling KCC2 with ab259969 at 1/100 dilution (5.55 μg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 μg/ml)(Green). The nuclear counterstain was DAPI (Blue). No staining on rat liver.

Negative control : liver (PMID : 17715129) is observed.

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 μg/ml).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)

This data was developed using ab259969, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labelling KCC2 with ab259969 at 1/5000 dilution (0.111 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab259969 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Negative control : almost no staining on mouse liver.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)

This data was developed using ab259969, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labelling KCC2 with ab259969 at 1/5000 dilution (0.111 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum (PMID : 17715129). The section was incubated with ab259969 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Frozen sections) - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)

This data was developed using ab259969, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum (fresh) tissue labeling KCC2 with ab259969 at 1/100 dilution (5.55 μg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 μg/ml)(Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 μg/ml).

Immunohistochemistry (Frozen sections) - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)

This data was developed using ab259969, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum (fresh) tissue labeling KCC2 with ab259969 at 1/100 (5.55 μg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 μg/ml)(Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 μg/ml).

Immunocytochemistry/ Immunofluorescence - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)

This data was developed using ab259969, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cells labelling KCC2 with ab259969 at 1/200 dilution (2.775 μg/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 μg/ml)(Green). ab11267 Anti-MAP2 mouse monoclonal antibody at 1/500 dilution (4 μg/ml) followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution (2 μg/ml) was used to counterstain tubulin (Red). The Nuclear counterstain was DAPI (Blue).

Confocal image showing positive staining in rat primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 μg/ml).

Negative control 1 : ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution (2 μg/ml).

Negative control 2 : ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 μg/ml).

Flow Cytometry (Intracellular) - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)

Flow cytometric (intracellular) analysis of 4% paraformaldehyde-fixed Rat primary neuron cells labelling KCC2 with ab259969 at 1/500 dilution (Right) compared with Isotype control Rabbit monoclonal IgG (ab172730) (Left). Goat Anti-Rabbit IgG Alexa Fluor® 488 (ab150081) at 1/2000 dilution was used as the secondary antibody.

Immunoprecipitation - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)
  • IP

Supplier Data

Immunoprecipitation - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)

This data was developed using ab259969, the same antibody clone in a different buffer formulation.

KCC2 was immunoprecipitated from 0.35 mg mouse brain tissue lysate with ab259969 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259969 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse brain tissue lysate 10μg

Lane 2 : ab259969 IP in Mouse brain tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab259969 in mouse brain tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds

The observed 30 KD band is like the KCC2 C terminus fragment and the observed 300 KD band is the dimer KCC2, as described in the literature (PMID : 22854961).

All lanes:

Immunoprecipitation - Anti-KCC2 antibody [EPR24203-85] (<a href='/en-us/products/primary-antibodies/kcc2-antibody-epr24203-85-ab259969'>ab259969</a>)

Predicted band size: 126 kDa

Observed band size: 140 kDa

false

Immunoprecipitation - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)
  • IP

Supplier Data

Immunoprecipitation - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)

This data was developed using ab259969, the same antibody clone in a different buffer formulation.

KCC2 was immunoprecipitated from 0.35 mg rat brain tissue lysate with ab259969 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259969 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Rat brain tissue lysate 10μg

Lane 2 : ab259969 IP in Rat brain tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab259969 in rat brain tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds

The observed 30 KD band is like the KCC2 C terminus fragment and the observed 300 KD band is the dimer KCC2, as described in the literature (PMID : 22854961).

All lanes:

Immunoprecipitation - Anti-KCC2 antibody [EPR24203-85] (<a href='/en-us/products/primary-antibodies/kcc2-antibody-epr24203-85-ab259969'>ab259969</a>)

Predicted band size: 126 kDa

Observed band size: 140 kDa

false

Western blot - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)
  • WB

Lab

Western blot - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)

This data was developed using ab259969, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Samples are non-boiled as boiling may cause protein aggregates.

Exposure time : 15 seconds

All lanes:

Western blot - Anti-KCC2 antibody [EPR24203-85] (<a href='/en-us/products/primary-antibodies/kcc2-antibody-epr24203-85-ab259969'>ab259969</a>) at 1/1000 dilution

All lanes:

Human spinal cord at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/5000 dilution

Predicted band size: 126 kDa

Observed band size: 140 kDa

false

Western blot - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)
  • WB

Lab

Western blot - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)

This data was developed using ab259969, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Samples are non-boiled as boiling may cause protein aggregates.

The observed 23, 30 KD bands are like the KCC2 C terminus fragment and the observed 300 KD band is the dimer KCC2, as described in the literature (PMID : 22854961).

Exposure time : 26 seconds

All lanes:

Western blot - Anti-KCC2 antibody [EPR24203-85] (<a href='/en-us/products/primary-antibodies/kcc2-antibody-epr24203-85-ab259969'>ab259969</a>) at 1/1000 dilution

All lanes:

Mouse spinal cord tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 126 kDa

Observed band size: 140 kDa

false

Western blot - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)
  • WB

Lab

Western blot - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)

This data was developed using ab259969, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Samples are non-boiled as boiling may cause protein aggregates.

The observed 30 KD band is like the KCC2 C terminus fragment which has been described in the literature (PMID : 22854961).

Negative control : Kidney, liver (PMID : 10212246).

Exposure time : 6 seconds

All lanes:

Western blot - Anti-KCC2 antibody [EPR24203-85] (<a href='/en-us/products/primary-antibodies/kcc2-antibody-epr24203-85-ab259969'>ab259969</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse kidney tissue lysate at 20 µg

Lane 3:

Mouse liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 126 kDa

Observed band size: 140 kDa

false

Western blot - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)
  • WB

Lab

Western blot - Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free (AB283588)

This data was developed using ab259969, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Samples are non-boiled as boiling may cause protein aggregates.

The observed 30 KD band is like the KCC2 C terminus fragment which has been described in the literature (PMID : 22854961).

Negative control : Kidney, liver (PMID : 10212246).

Exposure time : 6 seconds

All lanes:

Western blot - Anti-KCC2 antibody [EPR24203-85] (<a href='/en-us/products/primary-antibodies/kcc2-antibody-epr24203-85-ab259969'>ab259969</a>) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 20 µg

Lane 2:

Rat spinal cord tissue lysate at 20 µg

Lane 3:

Rat kidney tissue lysate

Lane 4:

Rat liver tissue lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 126 kDa

Observed band size: 140 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24203-85

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, Flow Cyt (Intra), ICC/IF, IHC-Fr, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB303518

Anti-NKCC1 antibody [EPR26256-60]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKCC1 antibody [EPR26256-60] (AB303518)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB177487

Anti-NeuN antibody [EPR12763] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB177487)

  • 5
  • 74
Primary Antibodies

AB32454

Anti-MAP2 antibody - Neuronal Marker

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody - Neuronal Marker (AB32454)

  • 5
  • 35
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

ab283588 is the carrier-free version of ab259969.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KCC2 also known as the potassium-chloride cotransporter 2 or SLC12A5 is an important neuronal protein. This transporter weighs approximately 125 kDa and is predominantly found in the central nervous system especially in neurons. KCC2 plays a central role in maintaining the intracellular chloride ion concentration by extruding chloride ions out in exchange for potassium ions. Through this action it aids in establishing the inhibitory action of neurotransmitters like GABA and glycine.
Biological function summary

KCC2 contributes significantly to the regulation of neuronal excitability and synaptic transmission. It does not appear as part of a larger protein complex but functions as a homodimer. KCC2's activity helps determine the strength and efficacy of inhibitory synapses influencing processes such as synaptic plasticity and neural development. By managing chloride ion gradients KCC2 ensures proper inhibitory neurotransmission which is essential for balanced neural activity.

Pathways

KCC2 is important for the GABAergic neurotransmission pathway which is significant for inhibitory signaling in the nervous system. This transporter interacts closely with proteins like NKCC1 another cation-chloride cotransporter which has an opposing function in chloride ion movement. The GABAergic pathway relies on KCC2 for hyperpolarizing inhibition contributing to the regulation of neuronal networks and stabilizing membrane potential.

KCC2 malfunctions associate with epilepsy and neuropathic pain. Altered expression or dysfunction of KCC2 disrupts inhibitory control leading to abnormal hyperexcitability in neurons which is a characteristic of epilepsy. Moreover in neuropathic pain downregulation of KCC2 impairs glycinergic inhibition contributing to the pathophysiology of chronic pain states. In these conditions KCC2's interaction with proteins such as NKCC1 highlights its role in maintaining inhibitory balance and neural homeostasis.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl(-) homeostasis (PubMed : 12106695). As major extruder of intracellular chloride, it establishes the low neuronal Cl(-) levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition (By similarity). Involved in the regulation of dendritic spine formation and maturation (PubMed : 24668262).
See full target information SLC12A5
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com