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AB133753

Anti-KCHIP1 antibody [EPR7624]

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(1 Publication)

Rabbit Recombinant Monoclonal KCHIP1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KCHIP1, VABP, KCNIP1, A-type potassium channel modulatory protein KCNIP1, Kv channel-interacting protein 1, Potassium channel-interacting protein 1, Vesicle APC-binding protein, KChIP1

3 Images
Flow Cytometry (Intracellular) - Anti-KCHIP1 antibody [EPR7624] (AB133753)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-KCHIP1 antibody [EPR7624] (AB133753)

Intracellular flow cytometric analysis of permeabilized HepG2 cells labelling KCHIP1 with ab133753 at 1/10 (red) or a rabbit IgG negative control antibody (green).

Western blot - Anti-KCHIP1 antibody [EPR7624] (AB133753)
  • WB

Unknown

Western blot - Anti-KCHIP1 antibody [EPR7624] (AB133753)

All lanes:

Western blot - Anti-KCHIP1 antibody [EPR7624] (ab133753) at 1/1000 dilution

Lane 1:

Human small intestine lysate at 10 µg

Lane 2:

Human fetal heart tissue lysate at 10 µg

Lane 3:

BxPC-3 cell lysate at 10 µg

Lane 4:

Caco-2 cell lysate at 10 µg

Lane 5:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti Rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 26 kDa

Observed band size: 30 kDa

false

OI-RD Scanning - Anti-KCHIP1 antibody [EPR7624] (AB133753)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-KCHIP1 antibody [EPR7624] (AB133753)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-KCHIP1 antibody [EPR7624] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7624

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KCHIP1 also known as Potassium Channel Interacting Protein 1 or KCNIP1 is a protein of approximately 28 kDa. It is expressed mainly in the brain with significant presence in neurons. Mechanically KCHIP1 interacts with and modulates the activity of Kv4 potassium channels. These are responsible for transient A-type potassium currents which play a role in stabilizing the neuronal resting membrane potential and shaping action potentials.
Biological function summary

In neuronal environments KCHIP1 acts as a subunit of the Kv4 channel complex. Its binding influences the kinetic properties and surface expression of Kv4 channels affecting potassium ion conductance. By adjusting the availability and activity of these channels KCHIP1 regulates neuronal excitability and plasticity. This control is important in maintaining appropriate signaling within neural circuits and any dysfunction in this component may affect neuronal communication.

Pathways

Potassium channel modulation by KCHIP1 occurs in the regulation of membrane excitability and electrical signaling in neurons. This function intersects with the MAPK signaling pathway a critical pathway responsible for various cellular responses including growth and stress responses. KCHIP1's role within these pathways links it to proteins such as Kv4.2 and Kv4.3 emphasizing its function in regulating neuronal activity and responses.

KCHIP1 has associations with neurodegenerative diseases like Alzheimer's. Impaired function or expression of KCHIP1 can lead to dysregulation of potassium channel activity impacting neuronal cell signaling and health. Furthermore its potential link with the Alzheimer's-related protein tau indicates that KCHIP1 might be involved in processes underlying tauopathy which affects neuronal stability and function. Understanding these interactions provides insights into therapeutic targets for managing neurodegenerative conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels (PubMed : 10676964, PubMed : 11423117, PubMed : 17187064, PubMed : 34552243, PubMed : 34997220). Regulates channel density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner (PubMed : 10676964, PubMed : 11423117, PubMed : 17187064, PubMed : 34552243, PubMed : 34997220). In vitro, modulates KCND1/Kv4.1 and KCND2/Kv4.2 currents (PubMed : 34552243). Increases the presence of KCND2 at the cell surface (PubMed : 12829703).
See full target information KCNIP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 22:497 PubMed38796413

2024

Atox1 regulates macrophage polarization in intestinal inflammation via ROS-NLRP3 inflammasome pathway.

Applications

Unspecified application

Species

Unspecified reactive species

MingXian Chen,Yu Chen,Rui Fu,SaiYue Liu,HaiXia Li,TangBiao Shen
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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