Anti-KCNA antibody [EPR26383-85]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNA antibody [EPR26383-85] (AB313624)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling KCNA with ab313624 at 1/2000 (0.248 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cerebrum, enriched in neurons at the axon initial segment. (PMID : 16473933).The section was incubated with ab313624 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNA antibody [EPR26383-85] (AB313624)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling KCNA with ab313624 at 1/2000 (0.248 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression : No staining on hepatocytes of human liver.The section was incubated with ab313624 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNA antibody [EPR26383-85] (AB313624)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling KCNA with ab313624 at 1/4000 (0.124 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebrum, enriched in neurons at the axon initial segment.The section was incubated with ab313624 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNA antibody [EPR26383-85] (AB313624)

Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling KCNA with ab313624 at 1/4000 (0.124 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebellum, enriched in the terminal regions around Purkinje cells.The section was incubated with ab313624 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNA antibody [EPR26383-85] (AB313624)

Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labeling KCNA with ab313624 at 1/4000 (0.124 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebellum, enriched in the terminal regions around Purkinje cells (PMID : 11259775).The section was incubated with ab313624 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNA antibody [EPR26383-85] (AB313624)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling KCNA with ab313624 at 1/4000 (0.124 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum, enriched in neurons at the axon initial segment.The section was incubated with ab313624 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNA antibody [EPR26383-85] (AB313624)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling KCNA with ab313624 at 1/4000 (0.124 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on rat testis.The section was incubated with ab313624 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNA antibody [EPR26383-85] (AB313624)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling KCNA with ab313624 at 1/4000 (0.124 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression : No staining on hepatocytes of mouse liver.The section was incubated with ab313624 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-KCNA antibody [EPR26383-85] (AB313624)

Fluorescence multiplex immunohistochemical analysis of mouse cerebellum (formalin-fixed paraffin-embedded section).

Panel A : merged staining of anti-GABRA6 (magenta; Opal™690), anti-KCNA (green; Opal™520) and anti-DGKZ (gray; Opal™570) on mouse cerebellum.
Panel B : anti-GABRA6 staining granule cells in mouse cerebellum.
Panel C : anti-KCNA staining basket cells in mouse cerebellum.
Panel D : anti-DGKZ staining Purkinje cells in mouse cerebellum.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab300069 at a 1/500 dilution, ab313624 at a 1/5000 and ab239080 at a 1/5000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-KCNA antibody [EPR26383-85] (AB313624)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse cerebellum tissue staining Carbonic Anhydrase 8/CA8 with ab319156 at a 1 : 10000 (0.05 ug/ml) dilution, ab313624 anti-KCNA2 used at 1 : 4000 (0.124 ug/ml) dilution and ab307710 anti-KCND2 used at a 1 : 2000 (0.266 ug/ml) dilution.

Panel A : merged staining of anti-CA8 (magenta; Opal™690), anti-KCNA2 (green; Opal™520) and anti-KCND2 (gray; Opal™570) on mouse cerebellum.
Panel B : anti-CA8 staining the Purkinje cells in mouse cerebellum.
Panel C : anti-KCNA2 staining the basket cells in mouse cerebellum.
Panel D : anti-KCND2 staining the granule cell layer in mouse cerebellum.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab319156, ab313624 and ab307710 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-KCNA antibody [EPR26383-85] (AB313624)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat cerebellum tissue staining Carbonic Anhydrase 8/CA8 with ab319156 at a 1 : 10000 (0.05 ug/ml) dilution, ab313624 anti-KCNA2 used at 1 : 4000 (0.124 ug/ml) dilution and ab307710 anti-KCND2 used at a 1 : 2000 (0.266 ug/ml) dilution.

Panel A : merged staining of anti-CA8 (magenta; Opal™690), anti-KCNA2 (green; Opal™520) and anti-KCND2 (gray; Opal™570) on rat cerebellum.
Panel B : anti-CA8 staining the Purkinje cells in rat cerebellum.
Panel C : anti-KCNA2 staining the basket cells in rat cerebellum.
Panel D : anti-KCND2 staining the granule cell layer in rat cerebellum.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab319156, ab313624 and ab307710 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-KCNA antibody [EPR26383-85] (AB313624)

Multiplex immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections of Rat cerebellum tissue staining with ab317831 at 1/2000 dilution, ab313624 at 1/4000 dilution, ab307710 at 1/2000 dilution. Secondary used was Opal Polymer HRP Ms + Rb. Panel A : merged staining of anti-Ppp1r17 (magenta; Opal™690), anti-KCNA2 (green; Opal™520) and anti-KCND2 (gray; Opal™570) on rat cerebellum. Panel B : anti-Ppp1r17 staining the Purkinje cells in rat cerebellum. Panel C : anti-KCNA2 staining the basket cells in rat cerebellum. Panel D : anti-KCND2 staining the granule cell layer in rat cerebellum. Nuclear DNA was labeled with DAPI (shown in blue). The section was incubated in three rounds of staining : in the order of ab317831, ab313624 and ab307710 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-KCNA antibody [EPR26383-85] (AB313624)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse cerebellum tissue staining PKC gamma with ab317315 at a 1 : 5000 (0.1 µg/ml) dilution, KCNA2 with ab316324 at 1 : 4000 (0.124 µg/ml) dilution and KCND2 with ab307710 at a 1 : 2000 (0.266 µg/ml) dilution followed by secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-PKC gamma (green; Opal^™520), anti-KCNA2 (magenta; Opal^™690) and anti-KCND2 (gray; Opal^™570) on mouse cerebellum.
Panel B : anti-PKC gamma staining the Purkinje cells and neuropil in mouse cerebellum.
Panel C : anti-KCNA2 staining the basket cells in mouse cerebellum.
Panel D : anti-KCND2 staining the granule cell layer in mouse cerebellum.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab317315, ab313624 and ab307710 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-KCNA antibody [EPR26383-85] (AB313624)

Multiplex immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections of Mouse cerebellum tissue staining with ab317831 at 1/2000 dilution, ab313624 at 1/4000 dilution, ab307710 at 1/2000 dilution. Secondary used was Opal Polymer HRP Ms + Rb. Panel A : merged staining of anti-Ppp1r17 (magenta; Opal™690), anti-KCNA2 (green; Opal™520) and anti-KCND2 (gray; Opal™570) on mouse cerebellum. Panel B : anti-Ppp1r17 staining the Purkinje cells in mouse cerebellum. Panel C : anti-KCNA2 staining the basket cells in mouse cerebellum. Panel D : anti-KCND2 staining the granule cell layer in mouse cerebellum. Nuclear DNA was labeled with DAPI (shown in blue). The section was incubated in three rounds of staining : in the order of ab317831, ab313624 and ab307710 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-KCNA antibody [EPR26383-85] (AB313624)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Rat cerebellum tissue staining PKC gamma with ab317315 at a 1 : 5000 (0.1 µg/ml) dilution, KCNA2 with ab316324 at 1 : 4000 (0.124 µg/ml) dilution and KCND2 with ab307710 at a 1 : 2000 (0.266 µg/ml) dilution followed by secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-PKC gamma (green; Opal^™520), anti-KCNA2 (magenta; Opal^™690) and anti-KCND2 (gray; Opal^™570) on rat cerebellum.
Panel B : anti-PKC gamma staining the Purkinje cells and neuropil in rat cerebellum.
Panel C : anti-KCNA2 staining the basket cells in rat cerebellum.
Panel D : anti-KCND2 staining the granule cell layer in rat cerebellum.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab317315, ab313624 and ab307710 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-KCNA antibody [EPR26383-85] (AB313624)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : testis Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. The identity of the lower MW band at approximately 55kDa is unknown. Samples are non-boiled as boiling may cause protein aggregation. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-KCNA antibody [EPR26383-85] (ab313624) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 20 µg

Lane 2:

Rat testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 75 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-KCNA antibody [EPR26383-85] (AB313624)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : liver Samples are non-boiled as boiling may cause protein aggregation. The identity of the top bands above 250 kDa is unknown. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 125 seconds

All lanes:

Western blot - Anti-KCNA antibody [EPR26383-85] (ab313624) at 1/1000 dilution

Lane 1:

Mouse cerebellum tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Mouse liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 75 kDa

false

Exposure time: 125s

• WB

Supplier Data

Western blot - Anti-KCNA antibody [EPR26383-85] (AB313624)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody reacts with all mouse KCNA subunits and weakly cross-reacts with recombinant mouse KCNA6 and KCNA7.

In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

All lanes:

Western blot - Anti-KCNA antibody [EPR26383-85] (ab313624) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a His-tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a mouse KCNA1 expression vector containing a His-tag whole cell lysate at 20 µg

Lane 3:

His-tagged mouse KCNA2 recombinant protein at 10 ng

Lane 4:

293T cells transfected with a mouse KCNA3 expression vector containing a His-tag whole cell lysate at 20 µg

Lane 5:

293T cells transfected with a mouse KCNA4 expression vector containing a His-tag whole cell lysate at 20 µg

Lane 6:

293T cells transfected with a mouse KCNA5 expression vector containing a His-tag whole cell lysate at 20 µg

Lane 7:

293T cells transfected with a mouse KCNA6 expression vector containing a His-tag whole cell lysate at 20 µg

Lane 8:

293T cells transfected with a mouse KCNA7 expression vector containing a His-tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 56 kDa,23 kDa

false

Exposure time: 15s