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AB326350

Anti-KCNA2 antibody [C25]

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Rabbit Recombinant Monoclonal Kv1.2 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Transfected cell lysate - Human, Rat, Human, Mouse samples.

View Alternative Names

Potassium voltage-gated channel subfamily A member 2, NGK1, Voltage-gated K(+) channel HuKIV, Voltage-gated potassium channel HBK5, Voltage-gated potassium channel subunit Kv1.2, KCNA2

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-KCNA2 antibody [C25] (AB326350)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KCNA2 antibody [C25] (AB326350)

Immunocytochemistry/Immunofluorescence analysis. Tissue : Neuroblastoma cells (Kelly). Species : Human. Fixation : 4% PFA for 10 min at RT. Permeabilization : 0.15% TritonX for 15 min at RT. Blocking : 10% goat serum for 40 min at RT. Primary Antibody : ab326350 at 1 : 100 for 1 hour at RT. Secondary Antibody : Goat anti-Rabbit IgG-AlexaFluor488 (green) at 1 : 1000 for 1 hour at RT in the dark. Counterstain : DAPI (blue) nuclear stain at 1 : 1000 for 5 min at RT in the dark.

Immunocytochemistry/ Immunofluorescence - Anti-KCNA2 antibody [C25] (AB326350)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KCNA2 antibody [C25] (AB326350)

Immunocytochemistry/Immunofluorescence analysis. Tissue : Pheochromocytoma cells (PC-12). Species : Rat. Fixation : 4% PFA for 10 min at RT. Permeabilization : 0.15% TritonX for 15 min at RT. Blocking : 10% goat serum for 40 min at RT. Primary Antibody : ab326350 at 1 : 100 for 1 hour at RT. Secondary Antibody : Goat anti-Rabbit IgG-AlexaFluor488 (green) at 1 : 1000 for 1 hour at RT in the dark. Counterstain : DAPI (blue) nuclear stain at 1 : 1000 for 5 min at RT in the dark.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNA2 antibody [C25] (AB326350)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNA2 antibody [C25] (AB326350)

Immunohistochemistry analysis. Tissue : Brain. Species : Mouse. Fixation : Paraffin embedded, 4um thin. Blocking : 3% milk for 1 hour at RT. Primary Antibody : ab326350 at 1 : 100 for 1 hour at RT. Secondary Antibody : Goat anti-Rabbit IgG-HRP (H&L) at 1 : 100 for 45 min at RT. Counterstain : Hematoxylin (purple) nuclear stain at 1 : 10 for 5 min at RT. Color Development : DAB at 1 : 50 for 15 min at RT.

Western blot - Anti-KCNA2 antibody [C25] (AB326350)
  • WB

Supplier Data

Western blot - Anti-KCNA2 antibody [C25] (AB326350)

Western blot analysis showing detection of Rat KCNA2 protein in a Rat brain membrane lysate. Block : 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody : ab326350 at 1 : 1000 for 2 hours at RT. Secondary Antibody : Goat anti-Rabbit IgG-HRP (H&L) at 1 : 5000 for 1 hour at RT. Color Development : Chemiluminescent for HRP for 3 min at RT in the dark. Exposed 0.3 seconds.

All lanes:

Western blot - Anti-KCNA2 antibody [C25] (ab326350) at 1/1000 dilution

All lanes:

Rat brain membrane lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit IgG-HRP (H&L) at 1/5000 dilution

false

Exposure time: 0.3s

Western blot - Anti-KCNA2 antibody [C25] (AB326350)
  • WB

Supplier Data

Western blot - Anti-KCNA2 antibody [C25] (AB326350)

Western blot analysis showing detection of Human KCNA2 protein in a transient over-expression (OE) lysate. Block : 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody : ab326350 at 1 : 10000 for 2 hours at RT. Secondary Antibody : Goat anti-Rabbit IgG-HRP (H&L) at 1 : 5000 for 1 hour at RT. Color Development : Chemiluminescent for HRP for 30 sec at RT in the dark. Exposed 0.5 seconds.

All lanes:

Western blot - Anti-KCNA2 antibody [C25] (ab326350) at 1/10000 dilution

Lane 1:

HEK293T OE Lysate at 2.5 µg

Lane 2:

HEK293T Neg. Ctrl at 2.5 µg

Secondary

All lanes:

Goat anti-Rabbit IgG-HRP (H&L) at 1/5000 dilution

false

Exposure time: 0.5s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

C25

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes, primarily in the brain and the central nervous system, but also in the cardiovascular system. Prevents aberrant action potential firing and regulates neuronal output. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane (PubMed : 11211111, PubMed : 19912772, PubMed : 23769686, PubMed : 8495559). Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNA1, KCNA2, KCNA4, KCNA5, KCNA6, KCNA7, and possibly other family members as well; channel properties depend on the type of alpha subunits that are part of the channel (PubMed : 20220134, PubMed : 8495559). Channel properties are modulated by cytoplasmic beta subunits that regulate the subcellular location of the alpha subunits and promote rapid inactivation of delayed rectifier potassium channels. In vivo, membranes probably contain a mixture of heteromeric potassium channel complexes, making it difficult to assign currents observed in intact tissues to any particular potassium channel family member. Homotetrameric KCNA2 forms a delayed-rectifier potassium channel that opens in response to membrane depolarization, followed by slow spontaneous channel closure (PubMed : 19912772, PubMed : 23769686). In contrast, a heteromultimer formed by KCNA2 and KCNA4 shows rapid inactivation (PubMed : 8495559). Regulates neuronal excitability and plays a role as pacemaker in the regulation of neuronal action potentials (By similarity). KCNA2-containing channels play a presynaptic role and prevent hyperexcitability and aberrant action potential firing (By similarity). Response to toxins that are selective for KCNA2-containing potassium channels suggests that in Purkinje cells, dendritic subthreshold KCNA2-containing potassium channels prevent random spontaneous calcium spikes, suppressing dendritic hyperexcitability without hindering the generation of somatic action potentials, and thereby play an important role in motor coordination (By similarity). Plays a role in the induction of long-term potentiation of neuron excitability in the CA3 layer of the hippocampus (By similarity). May function as down-stream effector for G protein-coupled receptors and inhibit GABAergic inputs to basolateral amygdala neurons (By similarity). May contribute to the regulation of neurotransmitter release, such as gamma-aminobutyric acid (GABA) (By similarity). Contributes to the regulation of the axonal release of the neurotransmitter dopamine (By similarity). Reduced KCNA2 expression plays a role in the perception of neuropathic pain after peripheral nerve injury, but not acute pain (By similarity). Plays a role in the regulation of the time spent in non-rapid eye movement (NREM) sleep (By similarity).
See full target information KCNA2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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