Anti-KCNA5/KV1-5 antibody [EPR27249-502] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-KCNA5/KV1-5 antibody [EPR27249-502] - BSA and Azide free (AB324821)

This data was developed using ab322456, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Human lung and liver.

The identity of the lower MW band at approximately 60 kDa (in Lane 6) is unknown.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 1-4 : 37 seconds; Lane 5-7 : 70 seconds

All lanes:

Western blot - Anti-KCNA5/Kv1.5 antibody [EPR27249-502] (<a href='/en-us/products/primary-antibodies/kcna5-kv15-antibody-epr27249-502-ab322456'>ab322456</a>) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 40 µg

Lane 2:

Human eyeball tissue lysate at 40 µg

Lane 3:

Human hypothalamus tissue lysate at 40 µg

Lane 4:

Human pituitary tissue lysate at 40 µg

Lane 5:

Human striatum tissue lysate at 40 µg

Lane 6:

Human lung tissue lysate at 40 µg

Lane 7:

Human liver tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa,36 kDa

false

• WB

Supplier Data

Western blot - Anti-KCNA5/KV1-5 antibody [EPR27249-502] - BSA and Azide free (AB324821)

This data was developed using ab322456, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Rat lung and liver.

The identity of the lower MW band at approximately 60 kDa (in Lane 3) is unknown.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 1-2 : 37 seconds; Lane 3-4 : 70 seconds

All lanes:

Western blot - Anti-KCNA5/Kv1.5 antibody [EPR27249-502] (<a href='/en-us/products/primary-antibodies/kcna5-kv15-antibody-epr27249-502-ab322456'>ab322456</a>) at 1/1000 dilution

Lane 1:

Rat cerebellum tissue lysate at 40 µg

Lane 2:

Rat brain tissue lysate at 40 µg

Lane 3:

Rat lung tissue lysate at 40 µg

Lane 4:

Rat liver tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa,36 kDa

false