Rabbit Monoclonal KCNA5/KV1-5 antibody. Suitable for WB and reacts with Human, Rat samples.
Images
![Western blot - Anti-KCNA5/Kv1.5 antibody [EPR27249-502] (AB322456), expandable thumbnail](https://content.abcam.com/products/images/kcna5-kv15-antibody-epr27249-502-ab322456--western-blot-img439721.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-KCNA5/Kv1.5 antibody [EPR27249-502] (AB322456)")
![Western blot - Anti-KCNA5/Kv1.5 antibody [EPR27249-502] (AB322456), expandable thumbnail](https://content.abcam.com/products/images/kcna5-kv15-antibody-epr27249-502-ab322456--western-blot-img439720.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-KCNA5/Kv1.5 antibody [EPR27249-502] (AB322456)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-Fr | ICC/IF | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane. Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNA1, KCNA2, KCNA4, KCNA5, and possibly other family members as well; channel properties depend on the type of alpha subunits that are part of the channel (PubMed:12130714). Channel properties are modulated by cytoplasmic beta subunits that regulate the subcellular location of the alpha subunits and promote rapid inactivation (PubMed:12130714). Homotetrameric channels display rapid activation and slow inactivation (PubMed:12130714, PubMed:8505626). Required for normal electrical conduction including formation of the infranodal ventricular conduction system and normal action potential configuration, as a result of its interaction with XIRP2 (By similarity). May play a role in regulating the secretion of insulin in normal pancreatic islets.Isoform 2Exhibits a faster depolarization rate, reduced voltage-dependent recovery from inactivation and an excessive cumulative inactivation.
Alternative names
Potassium voltage-gated channel subfamily A member 5, HPCN1, Voltage-gated potassium channel HK2, Voltage-gated potassium channel subunit Kv1.5, KCNA5
Rabbit Monoclonal KCNA5/KV1-5 antibody. Suitable for WB and reacts with Human, Rat samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-KCNA5/Kv1.5 antibody [EPR27249-502] (ab322456), expandable thumbnail](https://content.abcam.com/products/images/kcna5-kv15-antibody-epr27249-502-ab322456--western-blot-img439721.jpg "Western blot - Anti-KCNA5/Kv1.5 antibody [EPR27249-502] (ab322456)")
Western blot - Anti-KCNA5/Kv1.5 antibody [EPR27249-502] (ab322456)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: Rat lung and liver.
The identity of the lower MW band at approximately 60 kDa (in Lane 3) is unknown.
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lane 1-2: 37 seconds; Lane 3-4: 70 seconds
All lanes: Western blot - Anti-KCNA5/Kv1.5 antibody [EPR27249-502] (ab322456) at 1/1000 dilution
Lane 1: Rat cerebellum tissue lysate at 40 µg
Lane 2: Rat brain tissue lysate at 40 µg
Lane 3: Rat lung tissue lysate at 40 µg
Lane 4: Rat liver tissue lysate at 40 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 75 kDa, 36 kDa
![Western blot - Anti-KCNA5/Kv1.5 antibody [EPR27249-502] (ab322456), expandable thumbnail](https://content.abcam.com/products/images/kcna5-kv15-antibody-epr27249-502-ab322456--western-blot-img439720.jpg "Western blot - Anti-KCNA5/Kv1.5 antibody [EPR27249-502] (ab322456)")
Western blot - Anti-KCNA5/Kv1.5 antibody [EPR27249-502] (ab322456)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: Human lung and liver.
The identity of the lower MW band at approximately 60 kDa (in Lane 6) is unknown.
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lane 1-4: 37 seconds; Lane 5-7: 70 seconds
All lanes: Western blot - Anti-KCNA5/Kv1.5 antibody [EPR27249-502] (ab322456) at 1/1000 dilution
Lane 1: Human cerebellum tissue lysate at 40 µg
Lane 2: Human eyeball tissue lysate at 40 µg
Lane 3: Human hypothalamus tissue lysate at 40 µg
Lane 4: Human pituitary tissue lysate at 40 µg
Lane 5: Human striatum tissue lysate at 40 µg
Lane 6: Human lung tissue lysate at 40 µg
Lane 7: Human liver tissue lysate at 40 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 75 kDa, 36 kDa
Downloads
Product protocols
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