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AB203831

Anti-KCNIP4 antibody [EPR18314]

4

(1 Review)

|

(2 Publications)

Rabbit Recombinant Monoclonal KCNIP4 antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

View Alternative Names

CALP, KCHIP4, KCNIP4, Kv channel-interacting protein 4, KChIP4, A-type potassium channel modulatory protein 4, Calsenilin-like protein, Potassium channel-interacting protein 4

6 Images
Immunoprecipitation - Anti-KCNIP4 antibody [EPR18314] (AB203831)
  • IP

Supplier Data

Immunoprecipitation - Anti-KCNIP4 antibody [EPR18314] (AB203831)

KCNIP4 was immunoprecipitated from 1mg of Mouse brain whole cell lysate with ab203831 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab203831 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

Lane 1 : Mouse brain whole cell lysate 10ug (Input). Lane 2 : ab203831 IP in Mouse brain whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab203831 in Mouse brain whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-KCNIP4 antibody [EPR18314] (ab203831)

Predicted band size: 29 kDa

false

Western blot - Anti-KCNIP4 antibody [EPR18314] (AB203831)
  • WB

Supplier Data

Western blot - Anti-KCNIP4 antibody [EPR18314] (AB203831)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831) at 1/10000 dilution

All lanes:

Human midbrain lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 29 kDa

Observed band size: 28 kDa

false

Exposure time: 15s

Western blot - Anti-KCNIP4 antibody [EPR18314] (AB203831)
  • WB

Supplier Data

Western blot - Anti-KCNIP4 antibody [EPR18314] (AB203831)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831) at 1/10000 dilution

All lanes:

Human cerebellum lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 29 kDa

Observed band size: 28 kDa

false

Exposure time: 1s

Western blot - Anti-KCNIP4 antibody [EPR18314] (AB203831)
  • WB

Supplier Data

Western blot - Anti-KCNIP4 antibody [EPR18314] (AB203831)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Lane 3:

Human fetal kidney lysate at 10 µg

Lane 4:

Human fetal spleen lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 29 kDa

Observed band size: 28 kDa

false

Exposure time: 1min

Western blot - Anti-KCNIP4 antibody [EPR18314] (AB203831)
  • WB

Supplier Data

Western blot - Anti-KCNIP4 antibody [EPR18314] (AB203831)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831) at 1/1000 dilution

Lane 1:

Rat cerebellum tissue lysate at 10 µg

Lane 2:

Mouse cerebellum tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 29 kDa

Observed band size: 28 kDa

false

Exposure time: 15s

Western blot - Anti-KCNIP4 antibody [EPR18314] (AB203831)
  • WB

Supplier Data

Western blot - Anti-KCNIP4 antibody [EPR18314] (AB203831)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Mouse heart tissue lysate at 10 µg

Lane 3:

Mouse kidney tissue lysate at 10 µg

Lane 4:

Mouse spleen tissue lysate at 10 µg

Lane 5:

Rat brain tissue lysate at 10 µg

Lane 6:

Rat heart tissue lysate at 10 µg

Lane 7:

Rat kidney tissue lysate at 10 µg

Lane 8:

Rat spleen tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 29 kDa

Observed band size: 28 kDa

false

Exposure time: 15s

  • Carrier free

    Anti-KCNIP4 antibody [EPR18314] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18314

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Modulates KCND2 channel density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner (PubMed : 11847232, PubMed : 18957440, PubMed : 23576435). Modulates KCND3/Kv4.3 currents (PubMed : 23576435). Isoform 4 does not increase KCND2 expression at the cell membrane (PubMed : 18957440). Isoform 4 retains KCND3 in the endoplasmic reticulum and negatively regulates its expression at the cell membrane.
See full target information KCNIP4
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncology research 33:2379-2398 PubMed40918458

2025

Suppresses Colorectal Cancer Progression by Inhibiting Epithelial-Mesenchymal Transition via Regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhang Jiang,Yijun Xu,Qi Zhu,Yingxia Wu,Zhe Wang,Shuang He,Shiyong Yu,Honggang Xiang

PLoS genetics 16:e1008527 PubMed31999692

2020

Characterisation of canine KCNIP4: A novel gene for cerebellar ataxia identified by whole-genome sequencing two affected Norwegian Buhund dogs.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher A Jenkins,Lajos Kalmar,Kaspar Matiasek,Lorenzo Mari,Kaisa Kyöstilä,Hannes Lohi,Ellen C Schofield,Cathryn S Mellersh,Luisa De Risio,Sally L Ricketts
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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