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Rabbit Recombinant Monoclonal KCNIP4 antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.


Images

Western blot - Anti-KCNIP4 antibody [EPR18314] (AB203831), expandable thumbnail
  • Western blot - Anti-KCNIP4 antibody [EPR18314] (AB203831), expandable thumbnail
  • Western blot - Anti-KCNIP4 antibody [EPR18314] (AB203831), expandable thumbnail
  • Immunoprecipitation - Anti-KCNIP4 antibody [EPR18314] (AB203831), expandable thumbnail
  • Western blot - Anti-KCNIP4 antibody [EPR18314] (AB203831), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Expected
Tested
Mouse
Tested
Tested
Rat
Expected
Tested

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Target data

Function

Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Modulates KCND2 channel density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner (PubMed:11847232, PubMed:18957440, PubMed:23576435). Modulates KCND3/Kv4.3 currents (PubMed:23576435). Isoform 4 does not increase KCND2 expression at the cell membrane (PubMed:18957440). Isoform 4 retains KCND3 in the endoplasmic reticulum and negatively regulates its expression at the cell membrane.

Alternative names

CALP, KCHIP4, KCNIP4, Kv channel-interacting protein 4, KChIP4, A-type potassium channel modulatory protein 4, Calsenilin-like protein, Potassium channel-interacting protein 4

Recommended products

Rabbit Recombinant Monoclonal KCNIP4 antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR18314
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831), expandable thumbnail

    Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831) at 1/1000 dilution

    Lane 1: Human fetal brain lysate at 10 µg

    Lane 2: Human fetal heart lysate at 10 µg

    Lane 3: Human fetal kidney lysate at 10 µg

    Lane 4: Human fetal spleen lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 29 kDa

    Observed band size: 28 kDa

    Exposure time: 1min

  • Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831), expandable thumbnail

    Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 10 µg

    Lane 2: Mouse heart tissue lysate at 10 µg

    Lane 3: Mouse kidney tissue lysate at 10 µg

    Lane 4: Mouse spleen tissue lysate at 10 µg

    Lane 5: Rat brain tissue lysate at 10 µg

    Lane 6: Rat heart tissue lysate at 10 µg

    Lane 7: Rat kidney tissue lysate at 10 µg

    Lane 8: Rat spleen tissue lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 29 kDa

    Observed band size: 28 kDa

    Exposure time: 15s

  • Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831), expandable thumbnail

    Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831) at 1/1000 dilution

    Lane 1: Rat cerebellum tissue lysate at 10 µg

    Lane 2: Mouse cerebellum tissue lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 29 kDa

    Observed band size: 28 kDa

    Exposure time: 15s

  • Immunoprecipitation - Anti-KCNIP4 antibody [EPR18314] (ab203831), expandable thumbnail

    Immunoprecipitation - Anti-KCNIP4 antibody [EPR18314] (ab203831)

    KCNIP4 was immunoprecipitated from 1mg of Mouse brain whole cell lysate with ab203831 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab203831 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

    Lane 1: Mouse brain whole cell lysate 10ug (Input). Lane 2: ab203831 IP in Mouse brain whole cell lysate. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab203831 in Mouse brain whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    All lanes: Immunoprecipitation - Anti-KCNIP4 antibody [EPR18314] (ab203831)

    Predicted band size: 29 kDa

  • Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831), expandable thumbnail

    Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831) at 1/10000 dilution

    All lanes: Human cerebellum lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 29 kDa

    Observed band size: 28 kDa

    Exposure time: 1s

  • Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831), expandable thumbnail

    Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831) at 1/10000 dilution

    All lanes: Human midbrain lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 29 kDa

    Observed band size: 28 kDa

    Exposure time: 15s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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