Anti-KCNIP4 antibody [EPR18314] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-KCNIP4 antibody [EPR18314] - BSA and Azide free (AB251403)

This data was developed using ab203831, the same antibody clone in a different buffer formulation.

KCNIP4 was immunoprecipitated from 1mg of Mouse brain whole cell lysate with ab203831 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab203831 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

Lane 1 : Mouse brain whole cell lysate 10ug (Input). Lane 2 : ab203831 IP in Mouse brain whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab203831 in Mouse brain whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-KCNIP4 antibody [EPR18314] (<a href='/en-us/products/primary-antibodies/kcnip4-antibody-epr18314-ab203831'>ab203831</a>)

Predicted band size: 29 kDa

false

• WB

Supplier Data

Western blot - Anti-KCNIP4 antibody [EPR18314] - BSA and Azide free (AB251403)

This data was developed using ab203831, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KCNIP4 antibody [EPR18314] (<a href='/en-us/products/primary-antibodies/kcnip4-antibody-epr18314-ab203831'>ab203831</a>) at 1/10000 dilution

All lanes:

Human midbrain lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 29 kDa

Observed band size: 28 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-KCNIP4 antibody [EPR18314] - BSA and Azide free (AB251403)

This data was developed using ab203831, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KCNIP4 antibody [EPR18314] (<a href='/en-us/products/primary-antibodies/kcnip4-antibody-epr18314-ab203831'>ab203831</a>) at 1/10000 dilution

All lanes:

Human cerebellum lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 29 kDa

Observed band size: 28 kDa

false

Exposure time: 1s

• WB

Supplier Data

Western blot - Anti-KCNIP4 antibody [EPR18314] - BSA and Azide free (AB251403)

This data was developed using ab203831, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KCNIP4 antibody [EPR18314] (<a href='/en-us/products/primary-antibodies/kcnip4-antibody-epr18314-ab203831'>ab203831</a>) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Lane 3:

Human fetal kidney lysate at 10 µg

Lane 4:

Human fetal spleen lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 29 kDa

Observed band size: 28 kDa

false

Exposure time: 1min

• WB

Supplier Data

Western blot - Anti-KCNIP4 antibody [EPR18314] - BSA and Azide free (AB251403)

This data was developed using ab203831, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KCNIP4 antibody [EPR18314] (<a href='/en-us/products/primary-antibodies/kcnip4-antibody-epr18314-ab203831'>ab203831</a>) at 1/1000 dilution

Lane 1:

Rat cerebellum tissue lysate at 10 µg

Lane 2:

Mouse cerebellum tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 29 kDa

Observed band size: 28 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-KCNIP4 antibody [EPR18314] - BSA and Azide free (AB251403)

This data was developed using ab203831, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KCNIP4 antibody [EPR18314] (<a href='/en-us/products/primary-antibodies/kcnip4-antibody-epr18314-ab203831'>ab203831</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Mouse heart tissue lysate at 10 µg

Lane 3:

Mouse kidney tissue lysate at 10 µg

Lane 4:

Mouse spleen tissue lysate at 10 µg

Lane 5:

Rat brain tissue lysate at 10 µg

Lane 6:

Rat heart tissue lysate at 10 µg

Lane 7:

Rat kidney tissue lysate at 10 µg

Lane 8:

Rat spleen tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 29 kDa

Observed band size: 28 kDa

false

Exposure time: 15s