Rabbit Polyclonal KCNJ5 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human KCNJ5 aa 1-100.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human KCNJ5 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P48544
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Cow | Predicted | Predicted |
| Pig | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium.
Alternative names
GIRK4, KCNJ5, G protein-activated inward rectifier potassium channel 4, GIRK-4, Cardiac inward rectifier, Heart KATP channel, Inward rectifier K(+) channel Kir3.4, KATP-1, CIR, IRK-4
Recommended products
Rabbit Polyclonal KCNJ5 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human KCNJ5 aa 1-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human KCNJ5 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P48544
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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5 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNJ5 antibody (ab224396)
Paraffin-embedded human adrenal gland stained for KCNJ5 using ab224396 at 1/200 dilution in immunohistochemical analysis. Shows moderate to strong membranous positivity in glandular cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNJ5 antibody (ab224396)
Paraffin-embedded human skeletal muscle stained for KCNJ5 using ab224396 at 1/200 dilution in immunohistochemical analysis. Shows no positivity in myocytes as expected .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNJ5 antibody (ab224396)
Paraffin-embedded human skin stained for KCNJ5 using ab224396 at 1/200 dilution in immunohistochemical analysis. Shows no positivity in squamous epithelial cells as expected.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNJ5 antibody (ab224396)
Paraffin-embedded human pacreas stained for KCNJ5 using ab224396 at 1/200 dilution in immunohistochemical analysis. Shows moderate to strong membranous positivity in exocrine glandular cells.
Western blot - Anti-KCNJ5 antibody (ab224396)
All lanes: Western blot - Anti-KCNJ5 antibody (ab224396) at 1/100 dilution
Lane 1: Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate (negative control)
Lane 2: KCNJ5 overexpression HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Predicted band size: 48 kDa
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