Anti-KCNJ5 antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-KCNJ5 antibody (AB236756)

A549 (Human lung carcinoma cell line) cells stained for KCNJ5 (green) using ab236756 at 1/100 dilution in ICC/IF, followed by an Alexa-Fluor^®488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNJ5 antibody (AB236756)

Paraffin-embedded human adrenal gland tissue stained for KCNJ5 using ab236756 at 1/300 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.