Rabbit Recombinant Monoclonal KCNMB3 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 3 publications.
Images
![Western blot - Anti-KCNMB3 antibody [EPR9543(B)] (AB137041), expandable thumbnail](https://content.abcam.com/products/images/kcnmb3-antibody-epr9543b-ab137041--western-blot-img102025.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-KCNMB3 antibody [EPR9543(B)] (AB137041)")
![Flow Cytometry (Intracellular) - Anti-KCNMB3 antibody [EPR9543(B)] (AB137041), expandable thumbnail](https://content.abcam.com/products/images/kcnmb3-antibody-epr9543b-ab137041--flow-cytometry-intracellular-img26546.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-KCNMB3 antibody [EPR9543(B)] (AB137041)")
Publications
Filter by
- Scientific reports 12:220232022Mapping the functional expression of auxiliary subunits of K1.1 in glioblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdam Feher,Zoltán Pethő,Tibor G Szanto,Álmos Klekner,Gabor Tajti,Gyula Batta,Tibor Hortobágyi,Zoltan Varga,Albrecht Schwab,Gyorgy PanyiPubMed 36539587
- The Journal of physiology 595:4631-46452017Large-conductance Ca -activated K channel activation by apical P2Y receptor agonists requires hydrocortisone in differentiated airway epithelium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNathan A Zaidman et. al.PubMed 28481415
- Arthritis research & therapy 18:1032016Different expression of β subunits of the KCa1.1 channel by invasive and non-invasive human fibroblast-like synoviocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZoltán Pethő et. al.PubMed 27165430
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Tested | Not recommended | Not recommended | Expected |
| Rat | Not recommended | Tested | Not recommended | Not recommended | Expected |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Alters the functional properties of the current expressed by the KCNMA1 channel. Isoform 2, isoform 3 and isoform 4 partially inactivate the current of KCNBMA. Isoform 4 induces a fast and incomplete inactivation of KCNMA1 channel that is detectable only at large depolarizations. In contrast, isoform 1 does not induce detectable inactivation of KCNMA1. Two or more subunits of KCNMB3 are required to block the KCNMA1 tetramer.
Alternative names
KCNMB2, KCNMBL, KCNMB3, Calcium-activated potassium channel subunit beta-3, BK channel subunit beta-3, Charybdotoxin receptor subunit beta-3, K(VCA)beta-3, Maxi K channel subunit beta-3, Slo-beta-3, BKbeta3, Hbeta3
Recommended products
Rabbit Recombinant Monoclonal KCNMB3 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 3 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR9543(B)
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-KCNMB3 antibody [EPR9543(B)] (ab137041), expandable thumbnail](https://content.abcam.com/products/images/kcnmb3-antibody-epr9543b-ab137041--western-blot-img102025.jpg "Western blot - Anti-KCNMB3 antibody [EPR9543(B)] (ab137041)")
Western blot - Anti-KCNMB3 antibody [EPR9543(B)] (ab137041)
All lanes: Western blot - Anti-KCNMB3 antibody [EPR9543(B)] (ab137041) at 1/1000 dilution
Lane 1: BxPC3 cell lysate at 10 µg
Lane 2: HepG2 cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
Lane 4: C6 cell lysate at 10 µg
Lane 5: RAW 264.7 cell lysate at 10 µg
Lane 6: PC12 cell lysate at 10 µg
Lane 7: NIH 3T3 cell lysate at 10 µg
SecondaryAll lanes: HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 32 kDa
![Flow Cytometry (Intracellular) - Anti-KCNMB3 antibody [EPR9543(B)] (ab137041), expandable thumbnail](https://content.abcam.com/products/images/kcnmb3-antibody-epr9543b-ab137041--flow-cytometry-intracellular-img26546.jpg "Flow Cytometry (Intracellular) - Anti-KCNMB3 antibody [EPR9543(B)] (ab137041)")
Flow Cytometry (Intracellular) - Anti-KCNMB3 antibody [EPR9543(B)] (ab137041)
Intracellular Flow Cytometry analysis of permeabilized Hela cells labelling KCNMB3 with ab137041 at 1/10 dilution (Red) or a Rabbit IgG (negative control) (Green).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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