Rabbit Recombinant Monoclonal KCNMB3 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.
Images
![Western blot - Anti-KCNMB3 antibody [EPR9543(B)] - BSA and Azide free (AB248782), expandable thumbnail](https://content.abcam.com/products/images/kcnmb3-antibody-epr9543b-bsa-and-azide-free-ab248782--western-blot-img102025.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-KCNMB3 antibody [EPR9543(B)] - BSA and Azide free (AB248782)")
![Flow Cytometry (Intracellular) - Anti-KCNMB3 antibody [EPR9543(B)] - BSA and Azide free (AB248782), expandable thumbnail](https://content.abcam.com/products/images/kcnmb3-antibody-epr9543b-bsa-and-azide-free-ab248782--flow-cytometry-intracellular-img26546.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-KCNMB3 antibody [EPR9543(B)] - BSA and Azide free (AB248782)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Tested | Not recommended | Not recommended | Expected |
| Rat | Not recommended | Tested | Not recommended | Not recommended | Expected |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Alters the functional properties of the current expressed by the KCNMA1 channel. Isoform 2, isoform 3 and isoform 4 partially inactivate the current of KCNBMA. Isoform 4 induces a fast and incomplete inactivation of KCNMA1 channel that is detectable only at large depolarizations. In contrast, isoform 1 does not induce detectable inactivation of KCNMA1. Two or more subunits of KCNMB3 are required to block the KCNMA1 tetramer.
Alternative names
KCNMB2, KCNMBL, KCNMB3, Calcium-activated potassium channel subunit beta-3, BK channel subunit beta-3, Charybdotoxin receptor subunit beta-3, K(VCA)beta-3, Maxi K channel subunit beta-3, Slo-beta-3, BKbeta3, Hbeta3
Recommended products
Rabbit Recombinant Monoclonal KCNMB3 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR9543(B)
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248782 is the carrier-free version of Anti-KCNMB3 antibody [EPR9543(B)] ab137041.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-KCNMB3 antibody [EPR9543(B)] - BSA and Azide free (ab248782), expandable thumbnail](https://content.abcam.com/products/images/kcnmb3-antibody-epr9543b-bsa-and-azide-free-ab248782--western-blot-img102025.jpg "Western blot - Anti-KCNMB3 antibody [EPR9543(B)] - BSA and Azide free (ab248782)")
Western blot - Anti-KCNMB3 antibody [EPR9543(B)] - BSA and Azide free (ab248782)
This data was developed using Anti-KCNMB3 antibody [EPR9543(B)] ab137041, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-KCNMB3 antibody [EPR9543(B)] (Anti-KCNMB3 antibody [EPR9543(B)] ab137041) at 1/1000 dilution
Lane 1: BxPC3 cell lysate at 10 µg
Lane 2: HepG2 cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
Lane 4: C6 cell lysate at 10 µg
Lane 5: RAW 264.7 cell lysate at 10 µg
Lane 6: PC12 cell lysate at 10 µg
Lane 7: NIH 3T3 cell lysate at 10 µg
SecondaryAll lanes: HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 32 kDa
![Flow Cytometry (Intracellular) - Anti-KCNMB3 antibody [EPR9543(B)] - BSA and Azide free (ab248782), expandable thumbnail](https://content.abcam.com/products/images/kcnmb3-antibody-epr9543b-bsa-and-azide-free-ab248782--flow-cytometry-intracellular-img26546.jpg "Flow Cytometry (Intracellular) - Anti-KCNMB3 antibody [EPR9543(B)] - BSA and Azide free (ab248782)")
Flow Cytometry (Intracellular) - Anti-KCNMB3 antibody [EPR9543(B)] - BSA and Azide free (ab248782)
This data was developed using Anti-KCNMB3 antibody [EPR9543(B)] ab137041, the same antibody clone in a different buffer formulation.
Intracellular Flow Cytometry analysis of permeabilized Hela cells labelling KCNMB3 with Anti-KCNMB3 antibody [EPR9543(B)] ab137041 at 1/10 dilution (Red) or a Rabbit IgG (negative control) (Green).
Downloads
Product protocols
- Visit the general protocols
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