AB99457
Anti-KCNN2/SK2 antibody
4
(1 Review)
|
(4 Publications)
Goat Polyclonal KCNN2/SK2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications.
View Alternative Names
Small conductance calcium-activated potassium channel protein 2, SK2, SKCa 2, SKCa2, KCa2.2, KCNN2
1 Images
- WB
Unknown
Western blot - Anti-KCNN2/SK2 antibody (AB99457)
Primary incubation was 1 hour.
All lanes:
Western blot - Anti-KCNN2/SK2 antibody (ab99457) at 0.3 µg/mL
All lanes:
Human liver lysate, in RIPA buffer at 35 µg
Predicted band size: 64 kDa
Observed band size: 23 kDa,70 kDa
true
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Reactivity data
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Properties and storage information
Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
Product protocols
For this product, it's our understanding that no specific protocols are required. You can visit:
- Visit the General protocols
- Visit the Troubleshooting
Target data
Small conductance calcium-activated potassium channel that mediates the voltage-independent transmembrane transfer of potassium across the cell membrane through a constitutive interaction with calmodulin which binds the intracellular calcium allowing its opening (PubMed : 10991935, PubMed : 33242881, PubMed : 9287325). The current is characterized by a voltage-independent activation, an intracellular calcium concentration increase-dependent activation and a single-channel conductance of about 3 picosiemens (PubMed : 10991935). Also presents an inwardly rectifying current, thus reducing its already small outward conductance of potassium ions, which is particularly the case when the membrane potential displays positive values, above + 20 mV (PubMed : 10991935). The inward rectification could be due to a blockade of the outward current by intracellular divalent cations such as calcium and magnesium and could also be due to an intrinsic property of the channel pore, independent of intracellular divalent ions. There are three positively charged amino acids in the S6 transmembrane domain, close to the pore, that collectively control the conductance and rectification through an electrostatic mechanism. Additionally, electrostatic contributions from these residues also play an important role in determining the intrinsic open probability of the channel in the absence of calcium, affecting the apparent calcium affinity for activation. Forms an heteromeric complex with calmodulin, which is constitutively associated in a calcium-independent manner. Channel opening is triggered when calcium binds the calmodulin resulting in a rotary movement leading to the formation of the dimeric complex to open the gate (By similarity). Plays a role in the repolarization phase of cardiac action potential (PubMed : 13679367).
Publications (4)
Recent publications for all applications. Explore the full list and refine your search
Virologica Sinica 38:9-22 PubMed36007838
2022
Domesticated HERV-W env contributes to the activation of the small conductance Ca-activated K type 2 channels via decreased 5-HT4 receptor in recent-onset schizophrenia.
Applications
Unspecified application
Species
Unspecified reactive species
Xiulin Wu,Qiujin Yan,Lianzhong Liu,Xing Xue,Wei Yao,Xuhang Li,Wenshi Li,Shuang Ding,Yaru Xia,Dongyan Zhang,Fan Zhu
Cell reports 31:107596 PubMed32375046
2020
The Cation Channel TMEM63B Is an Osmosensor Required for Hearing.
Applications
Unspecified application
Species
Unspecified reactive species
Han Du,Chang Ye,Dan Wu,Yan-Yu Zang,Linqing Zhang,Chen Chen,Xue-Yan He,Jian-Jun Yang,Ping Hu,Zhengfeng Xu,Guoqiang Wan,Yun Stone Shi
Pediatric research 82:1080-1087 PubMed28902181
2017
Platelet-derived growth factor receptor alpha-positive cells: a new cell type in the human ureteropelvic junction.
Applications
Unspecified application
Species
Unspecified reactive species
Manuela Hunziker,Anne-Marie O'Donnell,Prem Puri
Nature immunology 12:1010-6 PubMed21892172
2011
The inflammasome adaptor ASC regulates the function of adaptive immune cells by controlling Dock2-mediated Rac activation and actin polymerization.
Applications
Unspecified application
Species
Unspecified reactive species
Sirish K Ippagunta,R K Subbarao Malireddi,Patrick J Shaw,Geoffrey A Neale,Lieselotte Vande Walle,Douglas R Green,Yoshinori Fukui,Mohamed Lamkanfi,Thirumala-Devi Kanneganti
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