AB288309

Anti-KCNN2/SK2 antibody [EPR25233-1] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal KCNN2/SK2 antibody. Carrier free. Suitable for ICC/IF, IHC-P, IHC-Fr and reacts with Mouse, Human, Transfected cell line samples.

View Alternative Names

Small conductance calcium-activated potassium channel protein 2, SK2, SKCa 2, SKCa2, KCa2.2, KCNN2

11 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN2/SK2 antibody [EPR25233-1] - BSA and Azide free (AB288309)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN2/SK2 antibody [EPR25233-1] - BSA and Azide free (AB288309)

This data was developed using ab288299, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T cells labelling KCNN2/SK2 with ab288299 at 1/2000 (0.26 ug/ml) followed by a ready to use LeicaDS9800 (Bond^®, Polymer Refine Detection) was used. Positive staining on (B) HEK-293T cells transfected with a KCNN2 expression vector containing a his tag. No staining on HEK-293T cells transfected with a KCNN1 and KCNN3 expression vector containing a his tag (A, C) and HEK-293T cells transfected with empty vector containing a his tag.The section was incubated with ab288299 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^®, Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN2/SK2 antibody [EPR25233-1] - BSA and Azide free (AB288309)

This data was developed using ab288299, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labelling KCNN2/SK2 with ab288299 at 1/500 (1.04 ug/ml) followed by a ready to use LeicaDS9800 (Bond^®, Polymer Refine Detection). Cytoplasmic staining on human skeletal muscle. The section was incubated with ab288299 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN2/SK2 antibody [EPR25233-1] - BSA and Azide free (AB288309)

This data was developed using ab288299, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labelling KCNN2/SK2 with ab288299 at 1/500 (1.04 ug/ml) followed by a ready to use LeicaDS9800 (Bond^®, Polymer Refine Detection). Cytoplasmic staining on human cardiac muscle (PMID : 13679367). The section was incubated with ab288299 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunocytochemistry/ Immunofluorescence - Anti-KCNN2/SK2 antibody [EPR25233-1] - BSA and Azide free (AB288309)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-KCNN2/SK2 antibody [EPR25233-1] - BSA and Azide free (AB288309)

This data was developed using ab288299, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T cells labelling KCNN2/SK2 with ab288299 at 1/50 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HEK-293T cells transfected with a human KCNN2 expression vector containing a myc tag, no staining in HEK-293T cells transfected with a human KCNN1 or KCNN3 expression vector containing a myc tag. Myc-Tag Mouse mAb (Alexa Fluor^® 647) was used to counterstain myc tag at 1/100 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN2/SK2 antibody [EPR25233-1] - BSA and Azide free (AB288309)

This data was developed using ab288299, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling KCNN2/SK2 with ab288299 at 1/500 (0.104 ug/ml) followed by a ready to use LeicaDS9800 (Bond^®, Polymer Refine Detection). Negative control : No staining on human spleen. The section was incubated with ab288299 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN2/SK2 antibody [EPR25233-1] - BSA and Azide free (AB288309)

This data was developed using ab288299, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling KCNN2/SK2 with ab288299 at 1/2000 (0.104 ug/ml) followed by a ready to use LeicaDS9800 (Bond^®, Polymer Refine Detection). Negative control : No staining on mouse spleen (PMID : 8781233). The section was incubated with ab288299 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^®, Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN2/SK2 antibody [EPR25233-1] - BSA and Azide free (AB288309)

This data was developed using ab288299, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labelling KCNN2/SK2 with ab288299 at 1/2000 (0.26 ug/ml) followed by a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection). Cytoplasmic staining on mouse cerebullum (PMID : 8781233).The section was incubated with ab288299 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Frozen sections) - Anti-KCNN2/SK2 antibody [EPR25233-1] - BSA and Azide free (AB288309)

IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-KCNN2/SK2 antibody [EPR25233-1] - BSA and Azide free (AB288309)

This data was developed using ab288299, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney (fresh) tissue labeling KCNN2/SK2 with ab288299 at 1/100 (5.2 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (Green). No staining on mouse kidney is observed. Negative control : kidney (PMID : 10991935). The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution.

IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-KCNN2/SK2 antibody [EPR25233-1] - BSA and Azide free (AB288309)

This data was developed using ab288299, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (fresh) tissue labeling KCNN2/SK2 with ab288299 at 1/100 (5.2 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (Green). Positive staining on mouse hippocampus. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-KCNN2/SK2 antibody [EPR25233-1] - BSA and Azide free (AB288309)

This data was developed using ab288299, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling KCNN2/SK2 with ab288299 at 1/50 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing positive staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain neuron-specific cytoskeletal protein at 1/500 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN2/SK2 antibody [EPR25233-1] - BSA and Azide free (AB288309)

This data was developed using ab288299, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labelling KCNN2/SK2 with ab288299 at 1/2000 (0.26 ug/ml) followed by a ready to use LeicaDS9800 (Bond^®, Polymer Refine Detection). Cytoplasmic staining on mouse hippocampus (PMID : 8781233). The section was incubated with ab288299 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Unconjugated

Anti-KCNN2/SK2 antibody [EPR25233-1]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25233-1

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Transfected cell line": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

ab288309 is the carrier-free version of ab288299.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Small conductance calcium-activated potassium channel that mediates the voltage-independent transmembrane transfer of potassium across the cell membrane through a constitutive interaction with calmodulin which binds the intracellular calcium allowing its opening (PubMed : 10991935, PubMed : 33242881, PubMed : 9287325). The current is characterized by a voltage-independent activation, an intracellular calcium concentration increase-dependent activation and a single-channel conductance of about 3 picosiemens (PubMed : 10991935). Also presents an inwardly rectifying current, thus reducing its already small outward conductance of potassium ions, which is particularly the case when the membrane potential displays positive values, above + 20 mV (PubMed : 10991935). The inward rectification could be due to a blockade of the outward current by intracellular divalent cations such as calcium and magnesium and could also be due to an intrinsic property of the channel pore, independent of intracellular divalent ions. There are three positively charged amino acids in the S6 transmembrane domain, close to the pore, that collectively control the conductance and rectification through an electrostatic mechanism. Additionally, electrostatic contributions from these residues also play an important role in determining the intrinsic open probability of the channel in the absence of calcium, affecting the apparent calcium affinity for activation. Forms an heteromeric complex with calmodulin, which is constitutively associated in a calcium-independent manner. Channel opening is triggered when calcium binds the calmodulin resulting in a rotary movement leading to the formation of the dimeric complex to open the gate (By similarity). Plays a role in the repolarization phase of cardiac action potential (PubMed : 13679367).

See full target information KCNN2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KCNN2/SK2 antibody [EPR25233-1] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN2/SK2 antibody [EPR25233-1] - BSA and Azide free (AB288309)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN2/SK2 antibody [EPR25233-1] - BSA and Azide free (AB288309)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN2/SK2 antibody [EPR25233-1] - BSA and Azide free (AB288309)

Immunocytochemistry/ Immunofluorescence - Anti-KCNN2/SK2 antibody [EPR25233-1] - BSA and Azide free (AB288309)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN2/SK2 antibody [EPR25233-1] - BSA and Azide free (AB288309)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN2/SK2 antibody [EPR25233-1] - BSA and Azide free (AB288309)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN2/SK2 antibody [EPR25233-1] - BSA and Azide free (AB288309)

Immunohistochemistry (Frozen sections) - Anti-KCNN2/SK2 antibody [EPR25233-1] - BSA and Azide free (AB288309)

Immunohistochemistry (Frozen sections) - Anti-KCNN2/SK2 antibody [EPR25233-1] - BSA and Azide free (AB288309)

Immunocytochemistry/ Immunofluorescence - Anti-KCNN2/SK2 antibody [EPR25233-1] - BSA and Azide free (AB288309)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNN2/SK2 antibody [EPR25233-1] - BSA and Azide free (AB288309)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Product promise