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AB84819

Anti-KCNQ1 antibody [N37A/10]

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(8 Publications)

Mouse Monoclonal KCNQ1 antibody. Suitable for IP, Flow Cyt, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Chinese hamster, Mouse, Rat, Human samples. Cited in 8 publications. Immunogen corresponding to Fusion protein corresponding to aa 1-150 of KCNQ1, fused to KCNQ1.

View Alternative Names

KCNA8, KCNA9, KVLQT1, KCNQ1, Potassium voltage-gated channel subfamily KQT member 1, IKs producing slow voltage-gated potassium channel subunit alpha KvLQT1, KQT-like 1, Voltage-gated potassium channel subunit Kv7.1

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNQ1 antibody [N37A/10] (AB84819)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNQ1 antibody [N37A/10] (AB84819)

ab84819 staining KCNQ1 in human hippocampal tissue by IHC-P (Bouin's fixative fixed paraffin embedded). The sample was incubated with primary antibody at 100 and with a fluorophore conjugated anti mouse secondary at 1/50 dilution.

Flow Cytometry - Anti-KCNQ1 antibody [N37A/10] (AB84819)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-KCNQ1 antibody [N37A/10] (AB84819)

Overlay histogram showing HEK293 cells stained with ab84819 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84819, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNQ1 antibody [N37A/10] (AB84819)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNQ1 antibody [N37A/10] (AB84819)

Immunohistochemical analysis of Mouse Brain labelled for KCNQ1 using ab84819. Fixation : 10% Formalin Solution for 12-24 hours at RT. Primary Antibody : Mouse Anti-KCNQ1 Monoclonal Antibody (ab84819) at 1 : 1000 for 1 hour at RT. Secondary Antibody : HRP/DAB Detection System : Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain : Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 μl for 5 minutes at RT.

Western blot - Anti-KCNQ1 antibody [N37A/10] (AB84819)
  • WB

Supplier Data

Western blot - Anti-KCNQ1 antibody [N37A/10] (AB84819)

All lanes:

Western blot - Anti-KCNQ1 antibody [N37A/10] (ab84819) at 1/1000 dilution

All lanes:

Human Cell lysates at 15 µg

Secondary

All lanes:

Sheep Anti-Mouse IgG: HRP for 1 hour at RT

Predicted band size: 74 kDa

false

Western blot - Anti-KCNQ1 antibody [N37A/10] (AB84819)
  • WB

Unknown

Western blot - Anti-KCNQ1 antibody [N37A/10] (AB84819)

All lanes:

Western blot - Anti-KCNQ1 antibody [N37A/10] (ab84819) at 1 µg/mL

All lanes:

Cell lysates prepared from CHO -T cells transfected with mink-KvLQT1

Predicted band size: 74 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

N37A/10

Isotype

IgG1

Carrier free

No

Reacts with

Human, Chinese hamster, Mouse, Rat

Applications

ICC/IF, Flow Cyt, IP, IHC-Fr, IHC-P, WB

applications

Immunogen

Fusion protein corresponding to aa 1-150 of KCNQ1, fused to KCNQ1. The exact immunogen used to generate this antibody is proprietary information.

P51787

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-10 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.1-1 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Chinese hamster": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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Product details

The clone number has been updated from S37A-10 to N37A/10, both clone numbers name the same antibody clone.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pore-forming subunit of the voltage-gated potassium (Kv) channel involved in the regulation of cardiomyocyte excitability and important in normal development and functions of myocardium, inner ear, stomach and colon (PubMed : 10646604, PubMed : 25441029). Associates with KCNE beta subunits that modulates current kinetics (PubMed : 10646604, PubMed : 11101505, PubMed : 19687231, PubMed : 8900283, PubMed : 9108097, PubMed : 9312006). Induces a voltage-dependent current by rapidly activating and slowly deactivating potassium-selective outward current (PubMed : 10646604, PubMed : 11101505, PubMed : 25441029, PubMed : 8900283, PubMed : 9108097, PubMed : 9312006). Promotes also a delayed voltage activated potassium current showing outward rectification characteristic (By similarity). During beta-adrenergic receptor stimulation, participates in cardiac repolarization by associating with KCNE1 to form the I(Ks) cardiac potassium current that increases the amplitude and slows down the activation kinetics of outward potassium current I(Ks) (By similarity) (PubMed : 10646604, PubMed : 11101505, PubMed : 8900283, PubMed : 9108097, PubMed : 9312006). Muscarinic agonist oxotremorine-M strongly suppresses KCNQ1/KCNE1 current (PubMed : 10713961). When associated with KCNE3, forms the potassium channel that is important for cyclic AMP-stimulated intestinal secretion of chloride ions (PubMed : 10646604). This interaction with KCNE3 is reduced by 17beta-estradiol, resulting in the reduction of currents (By similarity). During conditions of increased substrate load, maintains the driving force for proximal tubular and intestinal sodium ions absorption, gastric acid secretion, and cAMP-induced jejunal chloride ions secretion (By similarity). Allows the provision of potassium ions to the luminal membrane of the secretory canaliculus in the resting state as well as during stimulated acid secretion (By similarity). When associated with KCNE2, forms a heterooligomer complex leading to currents with an apparently instantaneous activation, a rapid deactivation process and a linear current-voltage relationship and decreases the amplitude of the outward current (PubMed : 11101505). When associated with KCNE4, inhibits voltage-gated potassium channel activity (PubMed : 19687231). When associated with KCNE5, this complex only conducts current upon strong and continued depolarization (PubMed : 12324418). Also forms a heterotetramer with KCNQ5; has a voltage-gated potassium channel activity (PubMed : 24855057). Binds with phosphatidylinositol 4,5-bisphosphate (PubMed : 25037568). KCNQ1-KCNE2 channel associates with Na(+)-coupled myo-inositol symporter in the apical membrane of choroid plexus epithelium and regulates the myo-inositol gradient between blood and cerebrospinal fluid with an impact on neuron excitability (By similarity).. Isoform 2. Non-functional alone but modulatory when coexpressed with the full-length isoform 1.
See full target information KCNQ1
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Lung 203:48 PubMed40100414

2025

Effect of Electronic Cigarette Vapour Exposure on Ca- and cAMP-Dependent Ion Transport in Human Airway Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ya Niu,Chung-Yin Yip,Ke-Wu Pan,Judith Choi-Wo Mak,Wing-Hung Ko

JCI insight 10: PubMed39786967

2025

A PAS-targeting hERG1 activator reduces arrhythmic events in Jervell and Lange-Nielsen syndrome patient-derived hiPSC-CMs.

Applications

Unspecified application

Species

Unspecified reactive species

Chiamaka U Ukachukwu,Eric N Jimenez-Vazquez,Shreya Salwi,Matthew Goodrich,Francisco G Sanchez-Conde,Kate M Orland,Abhilasha Jain,Lee L Eckhardt,Timothy J Kamp,David K Jones

Nature cardiovascular research 2:1291-1309 PubMed38665938

2023

Unexpected impairment of underpins reentrant arrhythmias in a knock-in swine model of Timothy syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Andreu Porta-Sánchez,Andrea Mazzanti,Carmen Tarifa,Deni Kukavica,Alessandro Trancuccio,Muhammad Mohsin,Elisa Zanfrini,Andrea Perota,Roberto Duchi,Kevin Hernandez-Lopez,Miguel Eduardo Jáuregui-Abularach,Valerio Pergola,Eugenio Fernandez,Rossana Bongianino,Elisa Tavazzani,Patrick Gambelli,Mirella Memmi,Simone Scacchi,Luca F Pavarino,Piero Colli Franzone,Giovanni Lentini,David Filgueiras-Rama,Cesare Galli,Demetrio Julián Santiago,Silvia G Priori

Cardiovascular therapeutics 2022:4366948 PubMed35387268

2022

Effects of Vitamin D Deficiency on the Function of the Cardiac Autonomic Nervous System in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xuemei Luo,Jie Xiong,Hong Cai,Runmei Zou,Fang Li,Yuwen Wang,Cheng Wang

Frontiers in physiology 12:761910 PubMed34744797

2021

KCNQ1 Potassium Channel Expressed in Human Sperm Is Involved in Sperm Motility, Acrosome Reaction, Protein Tyrosine Phosphorylation, and Ion Homeostasis During Capacitation.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Gao,Kun Li,Fei Liang,Jianmin Yu,Ajuan Liu,Ya Ni,Peibei Sun

EBioMedicine 54:102723 PubMed32259713

2020

Identification, clinical manifestation and structural mechanisms of mutations in AMPK associated cardiac glycogen storage disease.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Hu,Dong Hu,Liwen Liu,Daniel Barr,Yang Liu,Norma Balderrabano-Saucedo,Bo Wang,Feng Zhu,Yumei Xue,Shulin Wu,BaoLiang Song,Heather McManus,Katherine Murphy,Katherine Loes,Arnon Adler,Lorenzo Monserrat,Charles Antzelevitch,Michael H Gollob,Perry M Elliott,Hector Barajas-Martinez

Cardiovascular research 95:419-29 PubMed22739119

2012

Disease characterization using LQTS-specific induced pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Toru Egashira,Shinsuke Yuasa,Tomoyuki Suzuki,Yoshiyasu Aizawa,Hiroyuki Yamakawa,Tomohiro Matsuhashi,Yohei Ohno,Shugo Tohyama,Shinichiro Okata,Tomohisa Seki,Yusuke Kuroda,Kojiro Yae,Hisayuki Hashimoto,Tomofumi Tanaka,Fumiyuki Hattori,Toshiaki Sato,Shunichiro Miyoshi,Seiji Takatsuki,Mitsushige Murata,Junko Kurokawa,Tetsushi Furukawa,Naomasa Makita,Takeshi Aiba,Wataru Shimizu,Minoru Horie,Kaichiro Kamiya,Itsuo Kodama,Satoshi Ogawa,Keiichi Fukuda

British journal of pharmacology 165:2389-408 PubMed22014103

2011

Potassium and ANO1/ TMEM16A chloride channel profiles distinguish atypical and typical smooth muscle cells from interstitial cells in the mouse renal pelvis.

Applications

Unspecified application

Species

Unspecified reactive species

Javed Iqbal,Mary A Tonta,Retsu Mitsui,Qun Li,Michelle Kett,Jinhua Li,Helena C Parkington,Hikaru Hashitani,Richard J Lang
View all publications
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