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Mouse Monoclonal KCNQ1 antibody. Suitable for IP, Flow Cyt, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Chinese hamster, Mouse, Rat, Human samples. Cited in 5 publications. Immunogen corresponding to Fusion protein corresponding to aa 1-150 of KCNQ1, fused to KCNQ1.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNQ1 antibody [N37A/10] (AB84819), expandable thumbnail
  • Flow Cytometry - Anti-KCNQ1 antibody [N37A/10] (AB84819), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNQ1 antibody [N37A/10] (AB84819), expandable thumbnail
  • Western blot - Anti-KCNQ1 antibody [N37A/10] (AB84819), expandable thumbnail
  • Western blot - Anti-KCNQ1 antibody [N37A/10] (AB84819), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Fusion protein corresponding to aa 1-150 of KCNQ1, fused to KCNQ1. The exact immunogen used to generate this antibody is proprietary information. Database link P51787

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IFIHC-Fr
Human
Expected
Tested
Tested
Tested
Expected
Expected
Mouse
Expected
Predicted
Predicted
Predicted
Expected
Expected
Rat
Expected
Predicted
Predicted
Predicted
Expected
Expected
Chinese hamster
Expected
Predicted
Predicted
Predicted
Expected
Expected

Expected
Expected

Species

Chinese hamster, Mouse, Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1 µg for 106 Cells

Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species

Chinese hamster, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1-10 µg/mL

Notes

-

Predicted
Predicted

Species

Chinese hamster, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

0.1-1 µg/mL

Notes

-

Predicted
Predicted

Species

Chinese hamster, Mouse, Rat

Dilution info

-

Notes

-

Expected
Expected

Species

Chinese hamster, Mouse, Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Expected
Expected

Species

Chinese hamster, Mouse, Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Pore-forming subunit of the voltage-gated potassium (Kv) channel involved in the regulation of cardiomyocyte excitability and important in normal development and functions of myocardium, inner ear, stomach and colon (PubMed:10646604, PubMed:25441029). Associates with KCNE beta subunits that modulates current kinetics (PubMed:10646604, PubMed:11101505, PubMed:19687231, PubMed:8900283, PubMed:9108097, PubMed:9312006). Induces a voltage-dependent current by rapidly activating and slowly deactivating potassium-selective outward current (PubMed:10646604, PubMed:11101505, PubMed:25441029, PubMed:8900283, PubMed:9108097, PubMed:9312006). Promotes also a delayed voltage activated potassium current showing outward rectification characteristic (By similarity). During beta-adrenergic receptor stimulation, participates in cardiac repolarization by associating with KCNE1 to form the I(Ks) cardiac potassium current that increases the amplitude and slows down the activation kinetics of outward potassium current I(Ks) (By similarity) (PubMed:10646604, PubMed:11101505, PubMed:8900283, PubMed:9108097, PubMed:9312006). Muscarinic agonist oxotremorine-M strongly suppresses KCNQ1/KCNE1 current (PubMed:10713961). When associated with KCNE3, forms the potassium channel that is important for cyclic AMP-stimulated intestinal secretion of chloride ions (PubMed:10646604). This interaction with KCNE3 is reduced by 17beta-estradiol, resulting in the reduction of currents (By similarity). During conditions of increased substrate load, maintains the driving force for proximal tubular and intestinal sodium ions absorption, gastric acid secretion, and cAMP-induced jejunal chloride ions secretion (By similarity). Allows the provision of potassium ions to the luminal membrane of the secretory canaliculus in the resting state as well as during stimulated acid secretion (By similarity). When associated with KCNE2, forms a heterooligomer complex leading to currents with an apparently instantaneous activation, a rapid deactivation process and a linear current-voltage relationship and decreases the amplitude of the outward current (PubMed:11101505). When associated with KCNE4, inhibits voltage-gated potassium channel activity (PubMed:19687231). When associated with KCNE5, this complex only conducts current upon strong and continued depolarization (PubMed:12324418). Also forms a heterotetramer with KCNQ5; has a voltage-gated potassium channel activity (PubMed:24855057). Binds with phosphatidylinositol 4,5-bisphosphate (PubMed:25037568). KCNQ1-KCNE2 channel associates with Na(+)-coupled myo-inositol symporter in the apical membrane of choroid plexus epithelium and regulates the myo-inositol gradient between blood and cerebrospinal fluid with an impact on neuron excitability (By similarity).Isoform 2Non-functional alone but modulatory when coexpressed with the full-length isoform 1.

Alternative names

Recommended products

Mouse Monoclonal KCNQ1 antibody. Suitable for IP, Flow Cyt, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Chinese hamster, Mouse, Rat, Human samples. Cited in 5 publications. Immunogen corresponding to Fusion protein corresponding to aa 1-150 of KCNQ1, fused to KCNQ1.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Fusion protein corresponding to aa 1-150 of KCNQ1, fused to KCNQ1. The exact immunogen used to generate this antibody is proprietary information. Database link P51787
Clone number

N37A/10

Purification technique

Affinity purification Protein G

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

The clone number has been updated from S37A-10 to N37A/10, both clone numbers name the same antibody clone.

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5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNQ1 antibody [N37A/10] (ab84819), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNQ1 antibody [N37A/10] (ab84819)

    ab84819 staining KCNQ1 in human hippocampal tissue by IHC-P (Bouin's fixative fixed paraffin embedded). The sample was incubated with primary antibody at 100 and with a fluorophore conjugated anti mouse secondary at 1/50 dilution.

  • Flow Cytometry - Anti-KCNQ1 antibody [N37A/10] (ab84819), expandable thumbnail

    Flow Cytometry - Anti-KCNQ1 antibody [N37A/10] (ab84819)

    Overlay histogram showing HEK293 cells stained with ab84819 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84819, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNQ1 antibody [N37A/10] (ab84819), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNQ1 antibody [N37A/10] (ab84819)

    Immunohistochemical analysis of Mouse Brain labelled for KCNQ1 using ab84819. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-KCNQ1 Monoclonal Antibody (ab84819) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 μl for 5 minutes at RT.

  • Western blot - Anti-KCNQ1 antibody [N37A/10] (ab84819), expandable thumbnail

    Western blot - Anti-KCNQ1 antibody [N37A/10] (ab84819)

    All lanes: Western blot - Anti-KCNQ1 antibody [N37A/10] (ab84819) at 1/1000 dilution

    All lanes: Human Cell lysates at 15 µg

    Secondary

    All lanes: Sheep Anti-Mouse IgG: HRP for 1 hour at RT

    Predicted band size: 74 kDa

  • Western blot - Anti-KCNQ1 antibody [N37A/10] (ab84819), expandable thumbnail

    Western blot - Anti-KCNQ1 antibody [N37A/10] (ab84819)

    All lanes: Western blot - Anti-KCNQ1 antibody [N37A/10] (ab84819) at 1 µg/mL

    All lanes: Cell lysates prepared from CHO -T cells transfected with mink-KvLQT1

    Predicted band size: 74 kDa

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Product protocols

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