Mouse Monoclonal KCNQ1 antibody. Suitable for IP, Flow Cyt, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Chinese hamster, Mouse, Rat, Human samples. Cited in 5 publications. Immunogen corresponding to Fusion protein corresponding to aa 1-150 of KCNQ1, fused to KCNQ1.
IgG1
Mouse
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Liquid
Monoclonal
IP | Flow Cyt | WB | IHC-P | ICC/IF | IHC-Fr | |
---|---|---|---|---|---|---|
Human | Expected | Tested | Tested | Tested | Expected | Expected |
Mouse | Expected | Predicted | Predicted | Predicted | Expected | Expected |
Rat | Expected | Predicted | Predicted | Predicted | Expected | Expected |
Chinese hamster | Expected | Predicted | Predicted | Predicted | Expected | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Chinese hamster, Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1 µg for 106 Cells | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Chinese hamster, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1-10 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chinese hamster, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.1-1 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chinese hamster, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chinese hamster, Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chinese hamster, Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
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Pore-forming subunit of the voltage-gated potassium (Kv) channel involved in the regulation of cardiomyocyte excitability and important in normal development and functions of myocardium, inner ear, stomach and colon (PubMed:10646604, PubMed:25441029). Associates with KCNE beta subunits that modulates current kinetics (PubMed:10646604, PubMed:11101505, PubMed:19687231, PubMed:8900283, PubMed:9108097, PubMed:9312006). Induces a voltage-dependent current by rapidly activating and slowly deactivating potassium-selective outward current (PubMed:10646604, PubMed:11101505, PubMed:25441029, PubMed:8900283, PubMed:9108097, PubMed:9312006). Promotes also a delayed voltage activated potassium current showing outward rectification characteristic (By similarity). During beta-adrenergic receptor stimulation, participates in cardiac repolarization by associating with KCNE1 to form the I(Ks) cardiac potassium current that increases the amplitude and slows down the activation kinetics of outward potassium current I(Ks) (By similarity) (PubMed:10646604, PubMed:11101505, PubMed:8900283, PubMed:9108097, PubMed:9312006). Muscarinic agonist oxotremorine-M strongly suppresses KCNQ1/KCNE1 current (PubMed:10713961). When associated with KCNE3, forms the potassium channel that is important for cyclic AMP-stimulated intestinal secretion of chloride ions (PubMed:10646604). This interaction with KCNE3 is reduced by 17beta-estradiol, resulting in the reduction of currents (By similarity). During conditions of increased substrate load, maintains the driving force for proximal tubular and intestinal sodium ions absorption, gastric acid secretion, and cAMP-induced jejunal chloride ions secretion (By similarity). Allows the provision of potassium ions to the luminal membrane of the secretory canaliculus in the resting state as well as during stimulated acid secretion (By similarity). When associated with KCNE2, forms a heterooligomer complex leading to currents with an apparently instantaneous activation, a rapid deactivation process and a linear current-voltage relationship and decreases the amplitude of the outward current (PubMed:11101505). When associated with KCNE4, inhibits voltage-gated potassium channel activity (PubMed:19687231). When associated with KCNE5, this complex only conducts current upon strong and continued depolarization (PubMed:12324418). Also forms a heterotetramer with KCNQ5; has a voltage-gated potassium channel activity (PubMed:24855057). Binds with phosphatidylinositol 4,5-bisphosphate (PubMed:25037568). KCNQ1-KCNE2 channel associates with Na(+)-coupled myo-inositol symporter in the apical membrane of choroid plexus epithelium and regulates the myo-inositol gradient between blood and cerebrospinal fluid with an impact on neuron excitability (By similarity).Isoform 2Non-functional alone but modulatory when coexpressed with the full-length isoform 1.
KCNA8, KCNA9, KVLQT1, KCNQ1, KCNA8, KCNA9, KVLQT1, Potassium voltage-gated channel subfamily KQT member 1, IKs producing slow voltage-gated potassium channel subunit alpha KvLQT1, KQT-like 1, Voltage-gated potassium channel subunit Kv7.1
Mouse Monoclonal KCNQ1 antibody. Suitable for IP, Flow Cyt, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Chinese hamster, Mouse, Rat, Human samples. Cited in 5 publications. Immunogen corresponding to Fusion protein corresponding to aa 1-150 of KCNQ1, fused to KCNQ1.
IgG1
Mouse
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Liquid
Monoclonal
N37A/10
Affinity purification Protein G
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
The clone number has been updated from S37A-10 to N37A/10, both clone numbers name the same antibody clone.
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Terms & Conditions.
ab84819 staining KCNQ1 in human hippocampal tissue by IHC-P (Bouin's fixative fixed paraffin embedded). The sample was incubated with primary antibody at 100 and with a fluorophore conjugated anti mouse secondary at 1/50 dilution.
Overlay histogram showing HEK293 cells stained with ab84819 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84819, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Immunohistochemical analysis of Mouse Brain labelled for KCNQ1 using ab84819. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-KCNQ1 Monoclonal Antibody (ab84819) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 μl for 5 minutes at RT.
All lanes: Western blot - Anti-KCNQ1 antibody [N37A/10] (ab84819) at 1/1000 dilution
All lanes: Human Cell lysates at 15 µg
All lanes: Sheep Anti-Mouse IgG: HRP for 1 hour at RT
Predicted band size: 74 kDa
All lanes: Western blot - Anti-KCNQ1 antibody [N37A/10] (ab84819) at 1 µg/mL
All lanes: Cell lysates prepared from CHO -T cells transfected with mink-KvLQT1
Predicted band size: 74 kDa
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