Anti-KCNQ2 antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-KCNQ2 antibody (AB22897)

Immunfluorescent detection of KCNQ2 (green colour) in differentiated murine neural precursors using Rabbit polyclonal to KCNQ2 (ab22897). KCNQ2 labeling is seen in the tips of neurite extensions (such as the cell in the top left corner), along axons, dendrites and possibily spines. ab22897 (3.5μg/ml) was incubated overnight at 4°C. Secondary antibody used is goat anti-rabbit Alexa fluor 488 (1h at RT ). The red colour is MAP2 detection (Secondary used is goat anti-mouse Alexa fluor 568; 1h at RT). NB : The white centres are caused by high intensity to-pro-3 blue nuclear counterstain cross-colouring with the red and background green.

This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, France

• WB

Collaborator

Western blot - Anti-KCNQ2 antibody (AB22897)

Rabbit polyclonal to KCNQ2 (ab22897) was used in reducing conditions in WB but not in denaturing conditions (i.e. not heated).

All lanes:

Western blot - Anti-KCNQ2 antibody (ab22897) at 1 µg/mL

Lane 1:

Rat whole brain lysate at 40 µg

Lane 2:

Mouse whole brain lysate at 40 µg

Secondary

All lanes:

Anti-rabbit HRP at 1/20000 dilution

Predicted band size: 96 kDa

Observed band size: 95 kDa

true

This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, France

• WB

Unknown

Western blot - Anti-KCNQ2 antibody (AB22897)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab22897 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Abcam recommends loading unheated lysates. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

All lanes:

Western blot - Anti-KCNQ2 antibody (ab22897) at 1 µg/mL

Lane 1:

Brain (Rat) Tissue Lysate at 20 µg

Lane 2:

Brain (Mouse) Tissue Lysate at 20 µg

Lane 3:

Brain (Human) Tissue Lysate - adult normal tissue at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 96 kDa

Observed band size: 140 kDa,15 kDa,95 kDa

true

Exposure time: 20min